DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica
We assessed fungal diversity present in air and freshly deposited snow samples obtained from Livingston Island, Antarctica, using DNA metabarcoding through high throughput sequencing (HTS). A total of 740 m(3) of air were pumped through a 0.22 µm membrane. Snow obtained shortly after deposition was...
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ftpubmed:oai:pubmedcentral.nih.gov:7733504 2023-05-15T13:55:02+02:00 DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica Rosa, Luiz Henrique Pinto, Otávio Henrique Bezerra Šantl-Temkiv, Tina Convey, Peter Carvalho-Silva, Micheline Rosa, Carlos Augusto Câmara, Paulo E. A. S. 2020-12-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733504/ http://www.ncbi.nlm.nih.gov/pubmed/33311553 https://doi.org/10.1038/s41598-020-78630-6 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733504/ http://www.ncbi.nlm.nih.gov/pubmed/33311553 http://dx.doi.org/10.1038/s41598-020-78630-6 © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Sci Rep Article Text 2020 ftpubmed https://doi.org/10.1038/s41598-020-78630-6 2020-12-20T01:37:46Z We assessed fungal diversity present in air and freshly deposited snow samples obtained from Livingston Island, Antarctica, using DNA metabarcoding through high throughput sequencing (HTS). A total of 740 m(3) of air were pumped through a 0.22 µm membrane. Snow obtained shortly after deposition was kept at room temperature and yielded 3.760 L of water, which was filtered using Sterivex membranes of 0.22 µm mesh size. The total DNA present was extracted and sequenced. We detected 171 fungal amplicon sequence variants (ASVs), 70 from the air and 142 from the snow. They were dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Mucoromycota. Pseudogymnoascus, Cladosporium, Mortierella and Penicillium sp. were the most dominant ASVs detected in the air in rank order. In snow, Cladosporium, Pseudogymnoascus, Penicillium, Meyerozyma, Lecidea, Malassezia, Hanseniaspora, Austroplaca, Mortierella, Rhodotorula, Penicillium, Thelebolus, Aspergillus, Poaceicola, Glarea and Lecanora were the dominant ASVs present. In general, the two fungal assemblages displayed high diversity, richness, and dominance indices, with the assemblage found in snow having the highest diversity indices. Of the total fungal ASVs detected, 29 were only present in the air sample and 101 in the snow sample, with only 41 present in both samples; however, when only the dominant taxa from both samples were compared none occurred only in the air and, among the rare portion, 26 taxa occurred in both air and snow. Application of HTS revealed the presence of a more diverse fungal community in the air and snow of Livingston Island in comparison with studies using traditional isolation methods. The assemblages were dominated by cold-adapted and cosmopolitan fungal taxa, including members of the genera Pseudogymnoascus, Malassezia and Rhodotorula, which include some taxa reported as opportunistic. Our results support the hypothesis that the presence of microbiota in the airspora indicates the possibility of dispersal around Antarctica in the ... Text Antarc* Antarctica Livingston Island South Shetland Islands PubMed Central (PMC) Livingston Island ENVELOPE(-60.500,-60.500,-62.600,-62.600) South Shetland Islands Scientific Reports 10 1 |
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Article Rosa, Luiz Henrique Pinto, Otávio Henrique Bezerra Šantl-Temkiv, Tina Convey, Peter Carvalho-Silva, Micheline Rosa, Carlos Augusto Câmara, Paulo E. A. S. DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
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description |
We assessed fungal diversity present in air and freshly deposited snow samples obtained from Livingston Island, Antarctica, using DNA metabarcoding through high throughput sequencing (HTS). A total of 740 m(3) of air were pumped through a 0.22 µm membrane. Snow obtained shortly after deposition was kept at room temperature and yielded 3.760 L of water, which was filtered using Sterivex membranes of 0.22 µm mesh size. The total DNA present was extracted and sequenced. We detected 171 fungal amplicon sequence variants (ASVs), 70 from the air and 142 from the snow. They were dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota and Mucoromycota. Pseudogymnoascus, Cladosporium, Mortierella and Penicillium sp. were the most dominant ASVs detected in the air in rank order. In snow, Cladosporium, Pseudogymnoascus, Penicillium, Meyerozyma, Lecidea, Malassezia, Hanseniaspora, Austroplaca, Mortierella, Rhodotorula, Penicillium, Thelebolus, Aspergillus, Poaceicola, Glarea and Lecanora were the dominant ASVs present. In general, the two fungal assemblages displayed high diversity, richness, and dominance indices, with the assemblage found in snow having the highest diversity indices. Of the total fungal ASVs detected, 29 were only present in the air sample and 101 in the snow sample, with only 41 present in both samples; however, when only the dominant taxa from both samples were compared none occurred only in the air and, among the rare portion, 26 taxa occurred in both air and snow. Application of HTS revealed the presence of a more diverse fungal community in the air and snow of Livingston Island in comparison with studies using traditional isolation methods. The assemblages were dominated by cold-adapted and cosmopolitan fungal taxa, including members of the genera Pseudogymnoascus, Malassezia and Rhodotorula, which include some taxa reported as opportunistic. Our results support the hypothesis that the presence of microbiota in the airspora indicates the possibility of dispersal around Antarctica in the ... |
format |
Text |
author |
Rosa, Luiz Henrique Pinto, Otávio Henrique Bezerra Šantl-Temkiv, Tina Convey, Peter Carvalho-Silva, Micheline Rosa, Carlos Augusto Câmara, Paulo E. A. S. |
author_facet |
Rosa, Luiz Henrique Pinto, Otávio Henrique Bezerra Šantl-Temkiv, Tina Convey, Peter Carvalho-Silva, Micheline Rosa, Carlos Augusto Câmara, Paulo E. A. S. |
author_sort |
Rosa, Luiz Henrique |
title |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_short |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_full |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_fullStr |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_full_unstemmed |
DNA metabarcoding of fungal diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica |
title_sort |
dna metabarcoding of fungal diversity in air and snow of livingston island, south shetland islands, antarctica |
publisher |
Nature Publishing Group UK |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733504/ http://www.ncbi.nlm.nih.gov/pubmed/33311553 https://doi.org/10.1038/s41598-020-78630-6 |
long_lat |
ENVELOPE(-60.500,-60.500,-62.600,-62.600) |
geographic |
Livingston Island South Shetland Islands |
geographic_facet |
Livingston Island South Shetland Islands |
genre |
Antarc* Antarctica Livingston Island South Shetland Islands |
genre_facet |
Antarc* Antarctica Livingston Island South Shetland Islands |
op_source |
Sci Rep |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7733504/ http://www.ncbi.nlm.nih.gov/pubmed/33311553 http://dx.doi.org/10.1038/s41598-020-78630-6 |
op_rights |
© The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-020-78630-6 |
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Scientific Reports |
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10 |
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