Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon (Acipenser baerii)
Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon (Acipenser baerii) contains around 250 chromosomes and is...
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ftpubmed:oai:pubmedcentral.nih.gov:7699875 2023-05-15T13:01:40+02:00 Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon (Acipenser baerii) Biltueva, Larisa S. Prokopov, Dmitry Yu. Romanenko, Svetlana A. Interesova, Elena A. Schartl, Manfred Trifonov, Vladimir A. 2020-11-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699875/ http://www.ncbi.nlm.nih.gov/pubmed/33233736 https://doi.org/10.3390/genes11111375 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699875/ http://www.ncbi.nlm.nih.gov/pubmed/33233736 http://dx.doi.org/10.3390/genes11111375 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Genes (Basel) Article Text 2020 ftpubmed https://doi.org/10.3390/genes11111375 2020-12-06T02:02:09Z Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon (Acipenser baerii) contains around 250 chromosomes and is remarkable for the presence of paralogs from two rounds of whole-genome duplications (WGD). In this study, we applied the sterlet-derived acipenserid satDNA-based whole chromosome-specific probes to analyze the Siberian sturgeon karyotype. We demonstrate that the last genome duplication event in the Siberian sturgeon was accompanied by the simultaneous expansion of several repetitive DNA families. Some of the repetitive probes serve as good cytogenetic markers distinguishing paralogous chromosomes and detecting ancestral syntenic regions, which underwent fusions and fissions. The tendency of minisatellite specificity for chromosome size groups previously observed in the sterlet genome is also visible in the Siberian sturgeon. We provide an initial physical chromosome map of the Siberian sturgeon genome supported by molecular markers. The application of these data will facilitate genomic studies in other recent polyploid sturgeon species. Text Acipenser baerii Siberian sturgeon PubMed Central (PMC) Genes 11 11 1375 |
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Article Biltueva, Larisa S. Prokopov, Dmitry Yu. Romanenko, Svetlana A. Interesova, Elena A. Schartl, Manfred Trifonov, Vladimir A. Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon (Acipenser baerii) |
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Article |
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Polyploid genomes present a challenge for cytogenetic and genomic studies, due to the high number of similar size chromosomes and the simultaneous presence of hardly distinguishable paralogous elements. The karyotype of the Siberian sturgeon (Acipenser baerii) contains around 250 chromosomes and is remarkable for the presence of paralogs from two rounds of whole-genome duplications (WGD). In this study, we applied the sterlet-derived acipenserid satDNA-based whole chromosome-specific probes to analyze the Siberian sturgeon karyotype. We demonstrate that the last genome duplication event in the Siberian sturgeon was accompanied by the simultaneous expansion of several repetitive DNA families. Some of the repetitive probes serve as good cytogenetic markers distinguishing paralogous chromosomes and detecting ancestral syntenic regions, which underwent fusions and fissions. The tendency of minisatellite specificity for chromosome size groups previously observed in the sterlet genome is also visible in the Siberian sturgeon. We provide an initial physical chromosome map of the Siberian sturgeon genome supported by molecular markers. The application of these data will facilitate genomic studies in other recent polyploid sturgeon species. |
format |
Text |
author |
Biltueva, Larisa S. Prokopov, Dmitry Yu. Romanenko, Svetlana A. Interesova, Elena A. Schartl, Manfred Trifonov, Vladimir A. |
author_facet |
Biltueva, Larisa S. Prokopov, Dmitry Yu. Romanenko, Svetlana A. Interesova, Elena A. Schartl, Manfred Trifonov, Vladimir A. |
author_sort |
Biltueva, Larisa S. |
title |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon (Acipenser baerii) |
title_short |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon (Acipenser baerii) |
title_full |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon (Acipenser baerii) |
title_fullStr |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon (Acipenser baerii) |
title_full_unstemmed |
Chromosome Distribution of Highly Conserved Tandemly Arranged Repetitive DNAs in the Siberian Sturgeon (Acipenser baerii) |
title_sort |
chromosome distribution of highly conserved tandemly arranged repetitive dnas in the siberian sturgeon (acipenser baerii) |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699875/ http://www.ncbi.nlm.nih.gov/pubmed/33233736 https://doi.org/10.3390/genes11111375 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Genes (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7699875/ http://www.ncbi.nlm.nih.gov/pubmed/33233736 http://dx.doi.org/10.3390/genes11111375 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/genes11111375 |
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Genes |
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11 |
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11 |
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1375 |
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