Oxidative Chemical Stressors Alter the Physiological State of the Nasal Olfactory Mucosa of Atlantic Salmon
The olfactory organs of fish have vital functions for chemosensory and defence. Though there have been some ground-breaking discoveries of their involvement in immunity against pathogens in recent years, little is known about how they respond to non-infectious agents, such as exogenous oxidants, whi...
Published in: | Antioxidants |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
MDPI
2020
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698894/ http://www.ncbi.nlm.nih.gov/pubmed/33218110 https://doi.org/10.3390/antiox9111144 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:7698894 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:7698894 2023-05-15T15:32:01+02:00 Oxidative Chemical Stressors Alter the Physiological State of the Nasal Olfactory Mucosa of Atlantic Salmon Lazado, Carlo C. Voldvik, Vibeke Breiland, Mette W. Osório, João Hansen, Marianne H. S. Krasnov, Aleksei 2020-11-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698894/ http://www.ncbi.nlm.nih.gov/pubmed/33218110 https://doi.org/10.3390/antiox9111144 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698894/ http://www.ncbi.nlm.nih.gov/pubmed/33218110 http://dx.doi.org/10.3390/antiox9111144 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Antioxidants (Basel) Article Text 2020 ftpubmed https://doi.org/10.3390/antiox9111144 2020-12-06T01:59:32Z The olfactory organs of fish have vital functions for chemosensory and defence. Though there have been some ground-breaking discoveries of their involvement in immunity against pathogens in recent years, little is known about how they respond to non-infectious agents, such as exogenous oxidants, which fish encounter regularly. To this end, we employed Atlantic salmon (Salmo salar) as a model to study the molecular responses at the nasal olfactory mucosa of a teleost fish when challenged with oxidants. Microarray analysis was employed to unravel the transcriptional changes at the nasal olfactory mucosa following two types of in vivo exposure to peracetic acid (PAA), a highly potent oxidative agent commonly used in aquaculture: Trial 1: periodic and low dose (1 ppm, every 3 days over 45 days) to simulate a routine disinfection; and Trial 2: less frequent and high dose (10 ppm for 30 min, every 15 days, 3 times) to mimic a bath treatment. Furthermore, leukocytes from the olfactory organ were isolated and exposed to PAA, as well as to hydrogen peroxide (H(2)O(2)) and acetic acid (AA)—the two other components of PAA trade products—to perform targeted cellular and molecular response profiling. In the first trial, microarrays identified 32 differentially expressed genes (DEG) after a 45-day oxidant exposure. Erythrocyte-specific genes were overly represented and substantially upregulated following exogenous oxidant exposure. In Trial 2, in which a higher dose was administered, 62 DEGs were identified, over 80% of which were significantly upregulated after exposure. Genes involved in immune response, redox balance and stress, maintenance of cellular integrity and extracellular matrix were markedly affected by the oxidant. All chemical stimuli (i.e., PAA, H(2)O(2), AA) significantly affected the proliferation of nasal leukocytes, with indications of recovery observed in PAA- and H(2)O(2)-exposed cells. The migration of nasal leukocytes was promoted by H(2)O(2), but not much by PAA and AA. The three chemical oxidative ... Text Atlantic salmon Salmo salar PubMed Central (PMC) Paa ENVELOPE(-53.483,-53.483,66.017,66.017) Antioxidants 9 11 1144 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Lazado, Carlo C. Voldvik, Vibeke Breiland, Mette W. Osório, João Hansen, Marianne H. S. Krasnov, Aleksei Oxidative Chemical Stressors Alter the Physiological State of the Nasal Olfactory Mucosa of Atlantic Salmon |
topic_facet |
Article |
description |
The olfactory organs of fish have vital functions for chemosensory and defence. Though there have been some ground-breaking discoveries of their involvement in immunity against pathogens in recent years, little is known about how they respond to non-infectious agents, such as exogenous oxidants, which fish encounter regularly. To this end, we employed Atlantic salmon (Salmo salar) as a model to study the molecular responses at the nasal olfactory mucosa of a teleost fish when challenged with oxidants. Microarray analysis was employed to unravel the transcriptional changes at the nasal olfactory mucosa following two types of in vivo exposure to peracetic acid (PAA), a highly potent oxidative agent commonly used in aquaculture: Trial 1: periodic and low dose (1 ppm, every 3 days over 45 days) to simulate a routine disinfection; and Trial 2: less frequent and high dose (10 ppm for 30 min, every 15 days, 3 times) to mimic a bath treatment. Furthermore, leukocytes from the olfactory organ were isolated and exposed to PAA, as well as to hydrogen peroxide (H(2)O(2)) and acetic acid (AA)—the two other components of PAA trade products—to perform targeted cellular and molecular response profiling. In the first trial, microarrays identified 32 differentially expressed genes (DEG) after a 45-day oxidant exposure. Erythrocyte-specific genes were overly represented and substantially upregulated following exogenous oxidant exposure. In Trial 2, in which a higher dose was administered, 62 DEGs were identified, over 80% of which were significantly upregulated after exposure. Genes involved in immune response, redox balance and stress, maintenance of cellular integrity and extracellular matrix were markedly affected by the oxidant. All chemical stimuli (i.e., PAA, H(2)O(2), AA) significantly affected the proliferation of nasal leukocytes, with indications of recovery observed in PAA- and H(2)O(2)-exposed cells. The migration of nasal leukocytes was promoted by H(2)O(2), but not much by PAA and AA. The three chemical oxidative ... |
format |
Text |
author |
Lazado, Carlo C. Voldvik, Vibeke Breiland, Mette W. Osório, João Hansen, Marianne H. S. Krasnov, Aleksei |
author_facet |
Lazado, Carlo C. Voldvik, Vibeke Breiland, Mette W. Osório, João Hansen, Marianne H. S. Krasnov, Aleksei |
author_sort |
Lazado, Carlo C. |
title |
Oxidative Chemical Stressors Alter the Physiological State of the Nasal Olfactory Mucosa of Atlantic Salmon |
title_short |
Oxidative Chemical Stressors Alter the Physiological State of the Nasal Olfactory Mucosa of Atlantic Salmon |
title_full |
Oxidative Chemical Stressors Alter the Physiological State of the Nasal Olfactory Mucosa of Atlantic Salmon |
title_fullStr |
Oxidative Chemical Stressors Alter the Physiological State of the Nasal Olfactory Mucosa of Atlantic Salmon |
title_full_unstemmed |
Oxidative Chemical Stressors Alter the Physiological State of the Nasal Olfactory Mucosa of Atlantic Salmon |
title_sort |
oxidative chemical stressors alter the physiological state of the nasal olfactory mucosa of atlantic salmon |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698894/ http://www.ncbi.nlm.nih.gov/pubmed/33218110 https://doi.org/10.3390/antiox9111144 |
long_lat |
ENVELOPE(-53.483,-53.483,66.017,66.017) |
geographic |
Paa |
geographic_facet |
Paa |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Antioxidants (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7698894/ http://www.ncbi.nlm.nih.gov/pubmed/33218110 http://dx.doi.org/10.3390/antiox9111144 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/antiox9111144 |
container_title |
Antioxidants |
container_volume |
9 |
container_issue |
11 |
container_start_page |
1144 |
_version_ |
1766362512472670208 |