Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants
1-Aminociclopropane-1-carboxylate (ACC)-degrading bacteria having been widely studied for their use in alleviating abiotic stresses in plants. In the present study, we isolated and characterized ACC-degrading bacteria from the rhizosphere, phyllosphere, and endosphere of the Antarctic vascular plant...
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ftpubmed:oai:pubmedcentral.nih.gov:7697395 2023-05-15T13:46:03+02:00 Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants Araya, Macarena A. Valenzuela, Tamara Inostroza, Nitza G. Maruyama, Fumito Jorquera, Milko A. Acuña, Jacquelinne J. 2020-11-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697395/ http://www.ncbi.nlm.nih.gov/pubmed/33202619 https://doi.org/10.3390/microorganisms8111788 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697395/ http://www.ncbi.nlm.nih.gov/pubmed/33202619 http://dx.doi.org/10.3390/microorganisms8111788 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Microorganisms Article Text 2020 ftpubmed https://doi.org/10.3390/microorganisms8111788 2020-12-06T01:55:07Z 1-Aminociclopropane-1-carboxylate (ACC)-degrading bacteria having been widely studied for their use in alleviating abiotic stresses in plants. In the present study, we isolated and characterized ACC-degrading bacteria from the rhizosphere, phyllosphere, and endosphere of the Antarctic vascular plants Deschampsia antarctica and Colobanthus quitensis. One hundred and eighty of the 578 isolates (31%) were able to grow on minimal medium containing ACC, with 101 isolates (23, 37, and 41 endosphere-, phyllosphere- and rhizosphere-associated isolates, respectively) identified as being genetically unique by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Subsequently, freeze/thaw treatments and ice-recrystallization-inhibition (IRI) activity assays were performed, the results of which revealed that 77 (13%) of cold-tolerant isolates exhibited putative ACC deaminase activity. Significant (p ≤ 0.05) differences in IRI activity were also observed between the studied plant niches. Surprisingly, all the cold-tolerant isolates showed ACC deaminase activity, independent of the plant niches, with 12 isolates showing the highest ACC deaminase activities of 13.21–39.56 mmol α KB mg protein(−1) h(−1). These isolates were categorized as ‘cold-tolerant hyper-ACC-degrading bacteria’, and identified as members of Pseudomonas, Serratia, and Staphylococcus genera. The results revealed the occurrence of cold-tolerant hyper-ACC-degrading bacteria in diverse plant niches of Antarctic vascular plants, that could be investigated as novel microbial inoculants to alleviate abiotic stresses in plants. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic The Antarctic Microorganisms 8 11 1788 |
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Article Araya, Macarena A. Valenzuela, Tamara Inostroza, Nitza G. Maruyama, Fumito Jorquera, Milko A. Acuña, Jacquelinne J. Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants |
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Article |
description |
1-Aminociclopropane-1-carboxylate (ACC)-degrading bacteria having been widely studied for their use in alleviating abiotic stresses in plants. In the present study, we isolated and characterized ACC-degrading bacteria from the rhizosphere, phyllosphere, and endosphere of the Antarctic vascular plants Deschampsia antarctica and Colobanthus quitensis. One hundred and eighty of the 578 isolates (31%) were able to grow on minimal medium containing ACC, with 101 isolates (23, 37, and 41 endosphere-, phyllosphere- and rhizosphere-associated isolates, respectively) identified as being genetically unique by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Subsequently, freeze/thaw treatments and ice-recrystallization-inhibition (IRI) activity assays were performed, the results of which revealed that 77 (13%) of cold-tolerant isolates exhibited putative ACC deaminase activity. Significant (p ≤ 0.05) differences in IRI activity were also observed between the studied plant niches. Surprisingly, all the cold-tolerant isolates showed ACC deaminase activity, independent of the plant niches, with 12 isolates showing the highest ACC deaminase activities of 13.21–39.56 mmol α KB mg protein(−1) h(−1). These isolates were categorized as ‘cold-tolerant hyper-ACC-degrading bacteria’, and identified as members of Pseudomonas, Serratia, and Staphylococcus genera. The results revealed the occurrence of cold-tolerant hyper-ACC-degrading bacteria in diverse plant niches of Antarctic vascular plants, that could be investigated as novel microbial inoculants to alleviate abiotic stresses in plants. |
format |
Text |
author |
Araya, Macarena A. Valenzuela, Tamara Inostroza, Nitza G. Maruyama, Fumito Jorquera, Milko A. Acuña, Jacquelinne J. |
author_facet |
Araya, Macarena A. Valenzuela, Tamara Inostroza, Nitza G. Maruyama, Fumito Jorquera, Milko A. Acuña, Jacquelinne J. |
author_sort |
Araya, Macarena A. |
title |
Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants |
title_short |
Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants |
title_full |
Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants |
title_fullStr |
Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants |
title_full_unstemmed |
Isolation and Characterization of Cold-Tolerant Hyper-ACC-Degrading Bacteria from the Rhizosphere, Endosphere, and Phyllosphere of Antarctic Vascular Plants |
title_sort |
isolation and characterization of cold-tolerant hyper-acc-degrading bacteria from the rhizosphere, endosphere, and phyllosphere of antarctic vascular plants |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697395/ http://www.ncbi.nlm.nih.gov/pubmed/33202619 https://doi.org/10.3390/microorganisms8111788 |
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Antarctic The Antarctic |
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Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
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Antarc* Antarctic Antarctica |
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Microorganisms |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697395/ http://www.ncbi.nlm.nih.gov/pubmed/33202619 http://dx.doi.org/10.3390/microorganisms8111788 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
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https://doi.org/10.3390/microorganisms8111788 |
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Microorganisms |
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11 |
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1788 |
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