Molecular authentication of sika deer (Cervus nippon) based on allele-specific PCR

Sika deer (Cervus nippon) is listed as a tonic in many ancient Chinese pharmaceutical. Many adulterants of sika deer products have been found in Chinese medicinal materials markets, which led to detrimental impacts in clinical treatment. However, it is lack of the rapid and effective identification...

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Published in:Mitochondrial DNA Part B
Main Authors: Hou, Feixia, Gao, Jihai
Format: Text
Language:English
Published: Taylor & Francis 2019
Subjects:
Mito Communication
Rangifer tarandus
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687444/
https://doi.org/10.1080/23802359.2019.1624205
id ftpubmed:oai:pubmedcentral.nih.gov:7687444
record_format openpolar
spelling ftpubmed:oai:pubmedcentral.nih.gov:7687444 2023-05-15T18:04:18+02:00 Molecular authentication of sika deer (Cervus nippon) based on allele-specific PCR Hou, Feixia Gao, Jihai 2019-07-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687444/ https://doi.org/10.1080/23802359.2019.1624205 en eng Taylor & Francis http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687444/ http://dx.doi.org/10.1080/23802359.2019.1624205 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/ CC-BY Mitochondrial DNA B Resour Mito Communication Text 2019 ftpubmed https://doi.org/10.1080/23802359.2019.1624205 2020-12-27T01:19:35Z Sika deer (Cervus nippon) is listed as a tonic in many ancient Chinese pharmaceutical. Many adulterants of sika deer products have been found in Chinese medicinal materials markets, which led to detrimental impacts in clinical treatment. However, it is lack of the rapid and effective identification method for sika deer. This study amplified 574 bp fragment of mtDNA COI region of 19 samples from seven Cervidae species, and the relevant five sequences from reindeer (Rangifer tarandus) were downloaded from GenBank. It was found that there were two SNP loci for sika deer. Phylogenetic analysis indicated that individuals from sika deer clustered together. Based on SNP locus, one pair specific primer for allele-specific PCR identification of sika deer was designed, which could be used to rapidly and accurately identify sika deer. Text Rangifer tarandus PubMed Central (PMC) Mitochondrial DNA Part B 4 2 2231 2233
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Mito Communication
spellingShingle Mito Communication
Hou, Feixia
Gao, Jihai
Molecular authentication of sika deer (Cervus nippon) based on allele-specific PCR
topic_facet Mito Communication
description Sika deer (Cervus nippon) is listed as a tonic in many ancient Chinese pharmaceutical. Many adulterants of sika deer products have been found in Chinese medicinal materials markets, which led to detrimental impacts in clinical treatment. However, it is lack of the rapid and effective identification method for sika deer. This study amplified 574 bp fragment of mtDNA COI region of 19 samples from seven Cervidae species, and the relevant five sequences from reindeer (Rangifer tarandus) were downloaded from GenBank. It was found that there were two SNP loci for sika deer. Phylogenetic analysis indicated that individuals from sika deer clustered together. Based on SNP locus, one pair specific primer for allele-specific PCR identification of sika deer was designed, which could be used to rapidly and accurately identify sika deer.
format Text
author Hou, Feixia
Gao, Jihai
author_facet Hou, Feixia
Gao, Jihai
author_sort Hou, Feixia
title Molecular authentication of sika deer (Cervus nippon) based on allele-specific PCR
title_short Molecular authentication of sika deer (Cervus nippon) based on allele-specific PCR
title_full Molecular authentication of sika deer (Cervus nippon) based on allele-specific PCR
title_fullStr Molecular authentication of sika deer (Cervus nippon) based on allele-specific PCR
title_full_unstemmed Molecular authentication of sika deer (Cervus nippon) based on allele-specific PCR
title_sort molecular authentication of sika deer (cervus nippon) based on allele-specific pcr
publisher Taylor & Francis
publishDate 2019
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687444/
https://doi.org/10.1080/23802359.2019.1624205
genre Rangifer tarandus
genre_facet Rangifer tarandus
op_source Mitochondrial DNA B Resour
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7687444/
http://dx.doi.org/10.1080/23802359.2019.1624205
op_rights © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
http://creativecommons.org/licenses/by/4.0/
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/
op_rightsnorm CC-BY
op_doi https://doi.org/10.1080/23802359.2019.1624205
container_title Mitochondrial DNA Part B
container_volume 4
container_issue 2
container_start_page 2231
op_container_end_page 2233
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