Global distribution of a chlorophyll f cyanobacterial marker
Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging fr...
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ftpubmed:oai:pubmedcentral.nih.gov:7608106 2023-05-15T13:57:32+02:00 Global distribution of a chlorophyll f cyanobacterial marker Antonaru, Laura A. Cardona, Tanai Larkum, Anthony W. D. Nürnberg, Dennis J. 2020-05-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608106/ http://www.ncbi.nlm.nih.gov/pubmed/32457503 https://doi.org/10.1038/s41396-020-0670-y en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608106/ http://www.ncbi.nlm.nih.gov/pubmed/32457503 http://dx.doi.org/10.1038/s41396-020-0670-y © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY ISME J Article Text 2020 ftpubmed https://doi.org/10.1038/s41396-020-0670-y 2020-11-08T02:05:11Z Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype’s distribution. Text Antarc* Antarctic Arctic PubMed Central (PMC) Antarctic Arctic The ISME Journal 14 9 2275 2287 |
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Article Antonaru, Laura A. Cardona, Tanai Larkum, Anthony W. D. Nürnberg, Dennis J. Global distribution of a chlorophyll f cyanobacterial marker |
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Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype’s distribution. |
format |
Text |
author |
Antonaru, Laura A. Cardona, Tanai Larkum, Anthony W. D. Nürnberg, Dennis J. |
author_facet |
Antonaru, Laura A. Cardona, Tanai Larkum, Anthony W. D. Nürnberg, Dennis J. |
author_sort |
Antonaru, Laura A. |
title |
Global distribution of a chlorophyll f cyanobacterial marker |
title_short |
Global distribution of a chlorophyll f cyanobacterial marker |
title_full |
Global distribution of a chlorophyll f cyanobacterial marker |
title_fullStr |
Global distribution of a chlorophyll f cyanobacterial marker |
title_full_unstemmed |
Global distribution of a chlorophyll f cyanobacterial marker |
title_sort |
global distribution of a chlorophyll f cyanobacterial marker |
publisher |
Nature Publishing Group UK |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608106/ http://www.ncbi.nlm.nih.gov/pubmed/32457503 https://doi.org/10.1038/s41396-020-0670-y |
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Antarctic Arctic |
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Antarctic Arctic |
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Antarc* Antarctic Arctic |
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Antarc* Antarctic Arctic |
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ISME J |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7608106/ http://www.ncbi.nlm.nih.gov/pubmed/32457503 http://dx.doi.org/10.1038/s41396-020-0670-y |
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© The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
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https://doi.org/10.1038/s41396-020-0670-y |
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