Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome
Piscine reovirus (PRV) is the causative agent of heart and skeletal muscle inflammation (HSMI), which is detrimental to Atlantic Salmon (AS) aquaculture, but so far has not been cultivatable, which impedes studying the disease and developing a vaccine. Homogenates of head kidney and red blood cells...
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ftpubmed:oai:pubmedcentral.nih.gov:7601784 2023-05-15T15:32:30+02:00 Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome Pham, Phuc H. Misk, Ehab Papazotos, Fotini Jones, Ginny Polinski, Mark P. Contador, Elena Russell, Spencer Garver, Kyle A. Lumsden, John S. Bols, Niels C. 2020-10-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601784/ http://www.ncbi.nlm.nih.gov/pubmed/33053677 https://doi.org/10.3390/pathogens9100833 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601784/ http://www.ncbi.nlm.nih.gov/pubmed/33053677 http://dx.doi.org/10.3390/pathogens9100833 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Pathogens Article Text 2020 ftpubmed https://doi.org/10.3390/pathogens9100833 2020-11-08T01:54:16Z Piscine reovirus (PRV) is the causative agent of heart and skeletal muscle inflammation (HSMI), which is detrimental to Atlantic Salmon (AS) aquaculture, but so far has not been cultivatable, which impedes studying the disease and developing a vaccine. Homogenates of head kidney and red blood cells (RBC) from AS in which PRV-1 had been detected were applied to fish cell lines. The cell lines were from embryos, and from brain, blood, fin, gill, gonads, gut, heart, kidney, liver, skin, and spleen, and had the shapes of endothelial, epithelial, fibroblast, and macrophage cells. Most cell lines were derived from the Neopterygii subclass of fish, but one was from subclass Chondrostei. Cultures were examined by phase contrast microscopy for appearance, and by quantitative polymerase chain reaction (qPCR) for PRV-1 RNA amplification and for the capacity to transfer any changes to new cultures. No changes in appearance and Ct values were observed consistently or transferable to new cultures. Therefore, 31 cell lines examined were unable to support PRV-1 amplification and are described as belonging to the non-supportive PRV-1 invitrome. However, these investigations and cell lines can contribute to understanding PRV-1 cellular and host tropism, and the interactions between virus-infected and bystander cells. Text Atlantic salmon PubMed Central (PMC) Pathogens 9 10 833 |
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Article Pham, Phuc H. Misk, Ehab Papazotos, Fotini Jones, Ginny Polinski, Mark P. Contador, Elena Russell, Spencer Garver, Kyle A. Lumsden, John S. Bols, Niels C. Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
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Piscine reovirus (PRV) is the causative agent of heart and skeletal muscle inflammation (HSMI), which is detrimental to Atlantic Salmon (AS) aquaculture, but so far has not been cultivatable, which impedes studying the disease and developing a vaccine. Homogenates of head kidney and red blood cells (RBC) from AS in which PRV-1 had been detected were applied to fish cell lines. The cell lines were from embryos, and from brain, blood, fin, gill, gonads, gut, heart, kidney, liver, skin, and spleen, and had the shapes of endothelial, epithelial, fibroblast, and macrophage cells. Most cell lines were derived from the Neopterygii subclass of fish, but one was from subclass Chondrostei. Cultures were examined by phase contrast microscopy for appearance, and by quantitative polymerase chain reaction (qPCR) for PRV-1 RNA amplification and for the capacity to transfer any changes to new cultures. No changes in appearance and Ct values were observed consistently or transferable to new cultures. Therefore, 31 cell lines examined were unable to support PRV-1 amplification and are described as belonging to the non-supportive PRV-1 invitrome. However, these investigations and cell lines can contribute to understanding PRV-1 cellular and host tropism, and the interactions between virus-infected and bystander cells. |
format |
Text |
author |
Pham, Phuc H. Misk, Ehab Papazotos, Fotini Jones, Ginny Polinski, Mark P. Contador, Elena Russell, Spencer Garver, Kyle A. Lumsden, John S. Bols, Niels C. |
author_facet |
Pham, Phuc H. Misk, Ehab Papazotos, Fotini Jones, Ginny Polinski, Mark P. Contador, Elena Russell, Spencer Garver, Kyle A. Lumsden, John S. Bols, Niels C. |
author_sort |
Pham, Phuc H. |
title |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_short |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_full |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_fullStr |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_full_unstemmed |
Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome |
title_sort |
screening of fish cell lines for piscine orthoreovirus-1 (prv-1) amplification: identification of the non-supportive prv-1 invitrome |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601784/ http://www.ncbi.nlm.nih.gov/pubmed/33053677 https://doi.org/10.3390/pathogens9100833 |
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Atlantic salmon |
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Atlantic salmon |
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Pathogens |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601784/ http://www.ncbi.nlm.nih.gov/pubmed/33053677 http://dx.doi.org/10.3390/pathogens9100833 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/pathogens9100833 |
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Pathogens |
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