Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin
Type II collagen is an important component of cartilage; however, little is known about its effect on skin wound healing. In this study, type II collagen was extracted from the cartilage of Acipenser baerii and its effect on in vitro and in vivo wound healing was compared to type I collagen derived...
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ftpubmed:oai:pubmedcentral.nih.gov:7601416 2023-05-15T13:01:45+02:00 Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin Lai, Ching-Shu Tu, Chun-Wei Kuo, Hsing-Chun Sun, Pei-Pei Tsai, Mei-Ling 2020-10-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601416/ http://www.ncbi.nlm.nih.gov/pubmed/33050593 https://doi.org/10.3390/md18100511 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601416/ http://www.ncbi.nlm.nih.gov/pubmed/33050593 http://dx.doi.org/10.3390/md18100511 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Mar Drugs Article Text 2020 ftpubmed https://doi.org/10.3390/md18100511 2020-11-08T01:53:11Z Type II collagen is an important component of cartilage; however, little is known about its effect on skin wound healing. In this study, type II collagen was extracted from the cartilage of Acipenser baerii and its effect on in vitro and in vivo wound healing was compared to type I collagen derived from tilapia skin. Sturgeon cartilage collagen (SCC) was composed of α1 chains and with a thermal denaturation (T(d)) at 22.5 and melting temperature (T(m)) at 72.5 °C. Coating SCC potentiated proliferation, migration, and invasion of human dermal fibroblast adult (HDFa) cells. Furthermore, SCC upregulated the gene expression of extracellular matrix (ECM) components (col Iα1, col IIIα1, elastin, and Has2) and epithelial-mesenchymal transition (EMT) molecules (N-cadherin, Snail, and MMP-1) in HDFa. Pretreatment with Akt and mitogen-activated protein kinase (MAPK) inhibitors significantly attenuated the HDFa invasion caused by SCC. In mice, the application of SCC on dorsal wounds effectively facilitated wound healing as evidenced by 40–59% wound contraction, whereas the untreated wounds were 18%. We observed that SCC reduced inflammation, promoted granulation, tissue formation, and ECM deposition, as well as re-epithelialization in skin wounds. In addition, SCC markedly upregulated the production of growth factors in the dermis, and dermal and subcutaneous white adipose tissue; in contrast, the administration of tilapia skin collagen (TSC) characterized by typical type I collagen was mainly expressed in the epidermis. Collectively, these findings indicate SCC accelerated wound healing by targeting fibroblast in vitro and in vivo. Text Acipenser baerii PubMed Central (PMC) Marine Drugs 18 10 511 |
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Article Lai, Ching-Shu Tu, Chun-Wei Kuo, Hsing-Chun Sun, Pei-Pei Tsai, Mei-Ling Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin |
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Type II collagen is an important component of cartilage; however, little is known about its effect on skin wound healing. In this study, type II collagen was extracted from the cartilage of Acipenser baerii and its effect on in vitro and in vivo wound healing was compared to type I collagen derived from tilapia skin. Sturgeon cartilage collagen (SCC) was composed of α1 chains and with a thermal denaturation (T(d)) at 22.5 and melting temperature (T(m)) at 72.5 °C. Coating SCC potentiated proliferation, migration, and invasion of human dermal fibroblast adult (HDFa) cells. Furthermore, SCC upregulated the gene expression of extracellular matrix (ECM) components (col Iα1, col IIIα1, elastin, and Has2) and epithelial-mesenchymal transition (EMT) molecules (N-cadherin, Snail, and MMP-1) in HDFa. Pretreatment with Akt and mitogen-activated protein kinase (MAPK) inhibitors significantly attenuated the HDFa invasion caused by SCC. In mice, the application of SCC on dorsal wounds effectively facilitated wound healing as evidenced by 40–59% wound contraction, whereas the untreated wounds were 18%. We observed that SCC reduced inflammation, promoted granulation, tissue formation, and ECM deposition, as well as re-epithelialization in skin wounds. In addition, SCC markedly upregulated the production of growth factors in the dermis, and dermal and subcutaneous white adipose tissue; in contrast, the administration of tilapia skin collagen (TSC) characterized by typical type I collagen was mainly expressed in the epidermis. Collectively, these findings indicate SCC accelerated wound healing by targeting fibroblast in vitro and in vivo. |
format |
Text |
author |
Lai, Ching-Shu Tu, Chun-Wei Kuo, Hsing-Chun Sun, Pei-Pei Tsai, Mei-Ling |
author_facet |
Lai, Ching-Shu Tu, Chun-Wei Kuo, Hsing-Chun Sun, Pei-Pei Tsai, Mei-Ling |
author_sort |
Lai, Ching-Shu |
title |
Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin |
title_short |
Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin |
title_full |
Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin |
title_fullStr |
Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin |
title_full_unstemmed |
Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin |
title_sort |
type ii collagen from cartilage of acipenser baerii promotes wound healing in human dermal fibroblasts and in mouse skin |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601416/ http://www.ncbi.nlm.nih.gov/pubmed/33050593 https://doi.org/10.3390/md18100511 |
genre |
Acipenser baerii |
genre_facet |
Acipenser baerii |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601416/ http://www.ncbi.nlm.nih.gov/pubmed/33050593 http://dx.doi.org/10.3390/md18100511 |
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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
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https://doi.org/10.3390/md18100511 |
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Marine Drugs |
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18 |
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511 |
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1766285723882749952 |