Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus
Pilchard orthomyxovirus (POMV) is an emerging pathogen of concern to the salmon industry in Australia. To explore the molecular events that underpin POMV infection, we challenged Atlantic salmon (Salmo salar) post-smolts in seawater via cohabitation. Tissue samples of the head kidney and liver were...
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ftpubmed:oai:pubmedcentral.nih.gov:7600774 2023-05-15T15:30:46+02:00 Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus Samsing, Francisca Alexandre, Pamela Rigby, Megan Taylor, Richard S. Chong, Roger Wynne, James W. 2020-09-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600774/ http://www.ncbi.nlm.nih.gov/pubmed/33007914 https://doi.org/10.3390/pathogens9100807 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600774/ http://www.ncbi.nlm.nih.gov/pubmed/33007914 http://dx.doi.org/10.3390/pathogens9100807 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Pathogens Article Text 2020 ftpubmed https://doi.org/10.3390/pathogens9100807 2020-11-08T01:51:11Z Pilchard orthomyxovirus (POMV) is an emerging pathogen of concern to the salmon industry in Australia. To explore the molecular events that underpin POMV infection, we challenged Atlantic salmon (Salmo salar) post-smolts in seawater via cohabitation. Tissue samples of the head kidney and liver were collected from moribund and surviving individuals and analyzed using transcriptome sequencing. Viral loads were higher in the head kidney compared to the liver, yet the liver presented more upregulated genes. Fish infected with POMV showed a strong innate immune response that included the upregulation of pathogen recognition receptors such as RIG-I and Toll-like receptors as well as the induction of interferon-stimulated genes (MX, ISG15). Moribund fish also presented a dramatic induction of pro-inflammatory cytokines, contributing to severe tissue damage and morbidity. An induction of major histocompatibility complex (MHC) class I genes (B2M) and markers of T cell-mediated immunity (CD8-alpha, CD8-beta, Perforin-1, Granzyme-A) was observed in both moribund fish and survivors. In addition, differential connectivity analysis showed that three key regulators (RELA/p65, PRDM1, and HLF) related to cell-mediated immunity had significant differences in connectivity in “clinically healthy” versus “clinically affected” or moribund fish. Collectively, our results show that T cell-mediated immunity plays a central role in the response of Atlantic salmon to the infection with POMV. Text Atlantic salmon Salmo salar PubMed Central (PMC) Pathogens 9 10 807 |
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Article Samsing, Francisca Alexandre, Pamela Rigby, Megan Taylor, Richard S. Chong, Roger Wynne, James W. Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
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Pilchard orthomyxovirus (POMV) is an emerging pathogen of concern to the salmon industry in Australia. To explore the molecular events that underpin POMV infection, we challenged Atlantic salmon (Salmo salar) post-smolts in seawater via cohabitation. Tissue samples of the head kidney and liver were collected from moribund and surviving individuals and analyzed using transcriptome sequencing. Viral loads were higher in the head kidney compared to the liver, yet the liver presented more upregulated genes. Fish infected with POMV showed a strong innate immune response that included the upregulation of pathogen recognition receptors such as RIG-I and Toll-like receptors as well as the induction of interferon-stimulated genes (MX, ISG15). Moribund fish also presented a dramatic induction of pro-inflammatory cytokines, contributing to severe tissue damage and morbidity. An induction of major histocompatibility complex (MHC) class I genes (B2M) and markers of T cell-mediated immunity (CD8-alpha, CD8-beta, Perforin-1, Granzyme-A) was observed in both moribund fish and survivors. In addition, differential connectivity analysis showed that three key regulators (RELA/p65, PRDM1, and HLF) related to cell-mediated immunity had significant differences in connectivity in “clinically healthy” versus “clinically affected” or moribund fish. Collectively, our results show that T cell-mediated immunity plays a central role in the response of Atlantic salmon to the infection with POMV. |
format |
Text |
author |
Samsing, Francisca Alexandre, Pamela Rigby, Megan Taylor, Richard S. Chong, Roger Wynne, James W. |
author_facet |
Samsing, Francisca Alexandre, Pamela Rigby, Megan Taylor, Richard S. Chong, Roger Wynne, James W. |
author_sort |
Samsing, Francisca |
title |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_short |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_full |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_fullStr |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_full_unstemmed |
Transcriptome Response of Atlantic Salmon (Salmo salar) to a New Piscine Orthomyxovirus |
title_sort |
transcriptome response of atlantic salmon (salmo salar) to a new piscine orthomyxovirus |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600774/ http://www.ncbi.nlm.nih.gov/pubmed/33007914 https://doi.org/10.3390/pathogens9100807 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Pathogens |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600774/ http://www.ncbi.nlm.nih.gov/pubmed/33007914 http://dx.doi.org/10.3390/pathogens9100807 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/pathogens9100807 |
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Pathogens |
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