Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism...
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ftpubmed:oai:pubmedcentral.nih.gov:7595595 2023-05-15T15:58:25+02:00 Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas Picot, Sandy Faury, Nicole Arzul, Isabelle Chollet, Bruno Renault, Tristan Morga, Benjamin 2020-01-22 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595595/ http://www.ncbi.nlm.nih.gov/pubmed/31965890 https://doi.org/10.1080/15548627.2020.1713643 en eng Taylor & Francis http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595595/ http://www.ncbi.nlm.nih.gov/pubmed/31965890 http://dx.doi.org/10.1080/15548627.2020.1713643 © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. CC-BY-NC-ND Autophagy Research Paper Text 2020 ftpubmed https://doi.org/10.1080/15548627.2020.1713643 2020-11-15T01:31:55Z The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members of the C. gigas autophagy pathway. A total of 35 autophagy related proteins were found in the Pacific oyster. RACE-PCR was performed on several genes. Using molecular (real-time PCR) and protein-based (western blot and immunohistochemistry) approaches, the expression and localization of ATG12, ATG9, BECN1, MAP1LC3, MTOR, and SQSTM1, was investigated in different tissues of the Pacific oyster. Comparison with human and yeast counterparts demonstrated a high homology with the human autophagy pathway. The results also demonstrated that the key autophagy genes and their protein products were expressed in all the analyzed tissues of C. gigas. This study allows the characterization of the complete C. gigas autophagy pathway for the first time. Abbreviations: ATG: autophagy related; Atg1/ULK: unc-51 like autophagy activating kinase; ATG7: autophagy related 7; ATG9: autophagy related 9; ATG12: autophagy related 12; BECN1: beclin 1; BSA: bovine serum albumin; cDNA: complementary deoxyribonucleic acid; DNA: deoxyribonucleic acid; GABARAP: GABA type A receptor-associated protein; IHC: immunohistochemistry; MAP1LC3/LC3/Atg8: microtubule associated protein 1 light chain 3; MTOR: mechanistic target of rapamycin kinase; NCBI: national center for biotechnology information; ... Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Autophagy 16 11 2017 2035 |
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Research Paper |
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Research Paper Picot, Sandy Faury, Nicole Arzul, Isabelle Chollet, Bruno Renault, Tristan Morga, Benjamin Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
topic_facet |
Research Paper |
description |
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members of the C. gigas autophagy pathway. A total of 35 autophagy related proteins were found in the Pacific oyster. RACE-PCR was performed on several genes. Using molecular (real-time PCR) and protein-based (western blot and immunohistochemistry) approaches, the expression and localization of ATG12, ATG9, BECN1, MAP1LC3, MTOR, and SQSTM1, was investigated in different tissues of the Pacific oyster. Comparison with human and yeast counterparts demonstrated a high homology with the human autophagy pathway. The results also demonstrated that the key autophagy genes and their protein products were expressed in all the analyzed tissues of C. gigas. This study allows the characterization of the complete C. gigas autophagy pathway for the first time. Abbreviations: ATG: autophagy related; Atg1/ULK: unc-51 like autophagy activating kinase; ATG7: autophagy related 7; ATG9: autophagy related 9; ATG12: autophagy related 12; BECN1: beclin 1; BSA: bovine serum albumin; cDNA: complementary deoxyribonucleic acid; DNA: deoxyribonucleic acid; GABARAP: GABA type A receptor-associated protein; IHC: immunohistochemistry; MAP1LC3/LC3/Atg8: microtubule associated protein 1 light chain 3; MTOR: mechanistic target of rapamycin kinase; NCBI: national center for biotechnology information; ... |
format |
Text |
author |
Picot, Sandy Faury, Nicole Arzul, Isabelle Chollet, Bruno Renault, Tristan Morga, Benjamin |
author_facet |
Picot, Sandy Faury, Nicole Arzul, Isabelle Chollet, Bruno Renault, Tristan Morga, Benjamin |
author_sort |
Picot, Sandy |
title |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_short |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_full |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_fullStr |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_full_unstemmed |
Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas |
title_sort |
identification of the autophagy pathway in a mollusk bivalve, crassostrea gigas |
publisher |
Taylor & Francis |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595595/ http://www.ncbi.nlm.nih.gov/pubmed/31965890 https://doi.org/10.1080/15548627.2020.1713643 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Autophagy |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595595/ http://www.ncbi.nlm.nih.gov/pubmed/31965890 http://dx.doi.org/10.1080/15548627.2020.1713643 |
op_rights |
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by-nc-nd/4.0/ http://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1080/15548627.2020.1713643 |
container_title |
Autophagy |
container_volume |
16 |
container_issue |
11 |
container_start_page |
2017 |
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2035 |
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1766394157633372160 |