Gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the Antarctic clam
A computationally predicted gene regulatory network (GRN), generated from mantle-specific gene expression profiles in the Antarctic clam Laternula elliptica, was interrogated to test the regulation and interaction of duplicated inducible hsp70 paralogues. hsp70A and hsp70B were identified in the GRN...
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ftpubmed:oai:pubmedcentral.nih.gov:7591643 2023-05-15T13:37:41+02:00 Gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the Antarctic clam Ramsøe, Abigail Clark, Melody S. Sleight, Victoria A. 2020-05-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591643/ http://www.ncbi.nlm.nih.gov/pubmed/32436134 https://doi.org/10.1007/s12192-020-01118-9 en eng Springer Netherlands http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591643/ http://www.ncbi.nlm.nih.gov/pubmed/32436134 http://dx.doi.org/10.1007/s12192-020-01118-9 © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Cell Stress Chaperones Short Communication Text 2020 ftpubmed https://doi.org/10.1007/s12192-020-01118-9 2020-11-15T01:29:42Z A computationally predicted gene regulatory network (GRN), generated from mantle-specific gene expression profiles in the Antarctic clam Laternula elliptica, was interrogated to test the regulation and interaction of duplicated inducible hsp70 paralogues. hsp70A and hsp70B were identified in the GRN with each paralogue falling into unique submodules that were linked together by a single shared second neighbour. Annotations associated with the clusters in each submodule suggested that hsp70A primarily shares regulatory relationships with genes encoding ribosomal proteins, where it may have a role in protecting the ribosome under stress. hsp70B, on the other hand, interacted with a suite of genes involved in signalling pathways, including four transcription factors, cellular response to stress and the cytoskeleton. Given the contrasting submodules and associated annotations of the two hsp70 paralogues, the GRN analysis suggests that each gene is carrying out additional separate functions, as well as being involved in the traditional chaperone heat stress response, and therefore supports the hypothesis that subfunctionalization has occurred after gene duplication. The GRN was specifically produced from experiments investigating biomineralization; however, this study shows the utility of such data for investigating multiple questions concerning gene duplications, interactions and putative functions in a non-model species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12192-020-01118-9) contains supplementary material, which is available to authorized users. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Cell Stress and Chaperones 25 6 1111 1116 |
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Short Communication |
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Short Communication Ramsøe, Abigail Clark, Melody S. Sleight, Victoria A. Gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the Antarctic clam |
topic_facet |
Short Communication |
description |
A computationally predicted gene regulatory network (GRN), generated from mantle-specific gene expression profiles in the Antarctic clam Laternula elliptica, was interrogated to test the regulation and interaction of duplicated inducible hsp70 paralogues. hsp70A and hsp70B were identified in the GRN with each paralogue falling into unique submodules that were linked together by a single shared second neighbour. Annotations associated with the clusters in each submodule suggested that hsp70A primarily shares regulatory relationships with genes encoding ribosomal proteins, where it may have a role in protecting the ribosome under stress. hsp70B, on the other hand, interacted with a suite of genes involved in signalling pathways, including four transcription factors, cellular response to stress and the cytoskeleton. Given the contrasting submodules and associated annotations of the two hsp70 paralogues, the GRN analysis suggests that each gene is carrying out additional separate functions, as well as being involved in the traditional chaperone heat stress response, and therefore supports the hypothesis that subfunctionalization has occurred after gene duplication. The GRN was specifically produced from experiments investigating biomineralization; however, this study shows the utility of such data for investigating multiple questions concerning gene duplications, interactions and putative functions in a non-model species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12192-020-01118-9) contains supplementary material, which is available to authorized users. |
format |
Text |
author |
Ramsøe, Abigail Clark, Melody S. Sleight, Victoria A. |
author_facet |
Ramsøe, Abigail Clark, Melody S. Sleight, Victoria A. |
author_sort |
Ramsøe, Abigail |
title |
Gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the Antarctic clam |
title_short |
Gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the Antarctic clam |
title_full |
Gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the Antarctic clam |
title_fullStr |
Gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the Antarctic clam |
title_full_unstemmed |
Gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the Antarctic clam |
title_sort |
gene network analyses support subfunctionalization hypothesis for duplicated hsp70 genes in the antarctic clam |
publisher |
Springer Netherlands |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591643/ http://www.ncbi.nlm.nih.gov/pubmed/32436134 https://doi.org/10.1007/s12192-020-01118-9 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Cell Stress Chaperones |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591643/ http://www.ncbi.nlm.nih.gov/pubmed/32436134 http://dx.doi.org/10.1007/s12192-020-01118-9 |
op_rights |
© The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s12192-020-01118-9 |
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