Subsurface In Situ Detection of Microbes and Diverse Organic Matter Hotspots in the Greenland Ice Sheet
We used a deep-ultraviolet fluorescence mapping spectrometer, coupled to a drill system, to scan from the surface to 105 m depth into the Greenland ice sheet. The scan included firn and glacial ice and demonstrated that the instrument is able to determine small (mm) and large (cm) scale regions of o...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591382/ http://www.ncbi.nlm.nih.gov/pubmed/32700965 https://doi.org/10.1089/ast.2020.2241 |
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ftpubmed:oai:pubmedcentral.nih.gov:7591382 2023-05-15T16:28:00+02:00 Subsurface In Situ Detection of Microbes and Diverse Organic Matter Hotspots in the Greenland Ice Sheet Malaska, Michael J. Bhartia, Rohit Manatt, Kenneth S. Priscu, John C. Abbey, William J. Mellerowicz, Boleslaw Palmowski, Joseph Paulsen, Gale L. Zacny, Kris Eshelman, Evan J. D'Andrilli, Juliana 2020-10-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591382/ http://www.ncbi.nlm.nih.gov/pubmed/32700965 https://doi.org/10.1089/ast.2020.2241 en eng Mary Ann Liebert, Inc., publishers http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591382/ http://www.ncbi.nlm.nih.gov/pubmed/32700965 http://dx.doi.org/10.1089/ast.2020.2241 © Michael J. Malaska et al., 2020; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. CC-BY Astrobiology Research Articles Text 2020 ftpubmed https://doi.org/10.1089/ast.2020.2241 2020-11-01T02:03:10Z We used a deep-ultraviolet fluorescence mapping spectrometer, coupled to a drill system, to scan from the surface to 105 m depth into the Greenland ice sheet. The scan included firn and glacial ice and demonstrated that the instrument is able to determine small (mm) and large (cm) scale regions of organic matter concentration and discriminate spectral types of organic matter at high resolution. Both a linear point cloud scanning mode and a raster mapping mode were used to detect and localize microbial and organic matter “hotspots” embedded in the ice. Our instrument revealed diverse spectral signatures. Most hotspots were <20 mm in diameter, clearly isolated from other hotspots, and distributed stochastically; there was no evidence of layering in the ice at the fine scales examined (100 μm per pixel). The spectral signatures were consistent with organic matter fluorescence from microbes, lignins, fused-ring aromatic molecules, including polycyclic aromatic hydrocarbons, and biologically derived materials such as fulvic acids. In situ detection of organic matter hotspots in ice prevents loss of spatial information and signal dilution when compared with traditional bulk analysis of ice core meltwaters. Our methodology could be useful for detecting microbial and organic hotspots in terrestrial icy environments and on future missions to the Ocean Worlds of our Solar System. Text Greenland ice core Ice Sheet PubMed Central (PMC) Greenland Astrobiology 20 10 1185 1211 |
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Research Articles |
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Research Articles Malaska, Michael J. Bhartia, Rohit Manatt, Kenneth S. Priscu, John C. Abbey, William J. Mellerowicz, Boleslaw Palmowski, Joseph Paulsen, Gale L. Zacny, Kris Eshelman, Evan J. D'Andrilli, Juliana Subsurface In Situ Detection of Microbes and Diverse Organic Matter Hotspots in the Greenland Ice Sheet |
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Research Articles |
description |
We used a deep-ultraviolet fluorescence mapping spectrometer, coupled to a drill system, to scan from the surface to 105 m depth into the Greenland ice sheet. The scan included firn and glacial ice and demonstrated that the instrument is able to determine small (mm) and large (cm) scale regions of organic matter concentration and discriminate spectral types of organic matter at high resolution. Both a linear point cloud scanning mode and a raster mapping mode were used to detect and localize microbial and organic matter “hotspots” embedded in the ice. Our instrument revealed diverse spectral signatures. Most hotspots were <20 mm in diameter, clearly isolated from other hotspots, and distributed stochastically; there was no evidence of layering in the ice at the fine scales examined (100 μm per pixel). The spectral signatures were consistent with organic matter fluorescence from microbes, lignins, fused-ring aromatic molecules, including polycyclic aromatic hydrocarbons, and biologically derived materials such as fulvic acids. In situ detection of organic matter hotspots in ice prevents loss of spatial information and signal dilution when compared with traditional bulk analysis of ice core meltwaters. Our methodology could be useful for detecting microbial and organic hotspots in terrestrial icy environments and on future missions to the Ocean Worlds of our Solar System. |
format |
Text |
author |
Malaska, Michael J. Bhartia, Rohit Manatt, Kenneth S. Priscu, John C. Abbey, William J. Mellerowicz, Boleslaw Palmowski, Joseph Paulsen, Gale L. Zacny, Kris Eshelman, Evan J. D'Andrilli, Juliana |
author_facet |
Malaska, Michael J. Bhartia, Rohit Manatt, Kenneth S. Priscu, John C. Abbey, William J. Mellerowicz, Boleslaw Palmowski, Joseph Paulsen, Gale L. Zacny, Kris Eshelman, Evan J. D'Andrilli, Juliana |
author_sort |
Malaska, Michael J. |
title |
Subsurface In Situ Detection of Microbes and Diverse Organic Matter Hotspots in the Greenland Ice Sheet |
title_short |
Subsurface In Situ Detection of Microbes and Diverse Organic Matter Hotspots in the Greenland Ice Sheet |
title_full |
Subsurface In Situ Detection of Microbes and Diverse Organic Matter Hotspots in the Greenland Ice Sheet |
title_fullStr |
Subsurface In Situ Detection of Microbes and Diverse Organic Matter Hotspots in the Greenland Ice Sheet |
title_full_unstemmed |
Subsurface In Situ Detection of Microbes and Diverse Organic Matter Hotspots in the Greenland Ice Sheet |
title_sort |
subsurface in situ detection of microbes and diverse organic matter hotspots in the greenland ice sheet |
publisher |
Mary Ann Liebert, Inc., publishers |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591382/ http://www.ncbi.nlm.nih.gov/pubmed/32700965 https://doi.org/10.1089/ast.2020.2241 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
Greenland ice core Ice Sheet |
genre_facet |
Greenland ice core Ice Sheet |
op_source |
Astrobiology |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591382/ http://www.ncbi.nlm.nih.gov/pubmed/32700965 http://dx.doi.org/10.1089/ast.2020.2241 |
op_rights |
© Michael J. Malaska et al., 2020; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1089/ast.2020.2241 |
container_title |
Astrobiology |
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20 |
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10 |
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1185 |
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1211 |
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1766017616114089984 |