Hot in Cold: Microbial Life in the Hottest Springs in Permafrost
Chukotka is an arctic region located in the continuous permafrost zone, but thermal springs are abundant there. In this study, for the first time, the microbial communities of the Chukotka hot springs (CHS) biofilms and sediments with temperatures 54–94 °C were investigated and analyzed by NGS seque...
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ftpubmed:oai:pubmedcentral.nih.gov:7565842 2023-05-15T15:07:46+02:00 Hot in Cold: Microbial Life in the Hottest Springs in Permafrost Kochetkova, Tatiana V. Toshchakov, Stepan V. Zayulina, Kseniya S. Elcheninov, Alexander G. Zavarzina, Daria G. Lavrushin, Vasiliy Yu. Bonch-Osmolovskaya, Elizaveta A. Kublanov, Ilya V. 2020-08-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565842/ http://www.ncbi.nlm.nih.gov/pubmed/32867302 https://doi.org/10.3390/microorganisms8091308 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565842/ http://www.ncbi.nlm.nih.gov/pubmed/32867302 http://dx.doi.org/10.3390/microorganisms8091308 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Microorganisms Article Text 2020 ftpubmed https://doi.org/10.3390/microorganisms8091308 2020-11-01T01:31:43Z Chukotka is an arctic region located in the continuous permafrost zone, but thermal springs are abundant there. In this study, for the first time, the microbial communities of the Chukotka hot springs (CHS) biofilms and sediments with temperatures 54–94 °C were investigated and analyzed by NGS sequencing of 16S rRNA gene amplicons. In microbial mats (54–75 °C), phototrophic bacteria of genus Chloroflexus dominated (up to 89% of all prokaryotes), while Aquificae were the most numerous at higher temperatures in Fe-rich sediments and filamentous “streamers” (up to 92%). The electron donors typical for Aquificae, such as H(2)S and H(2), are absent or present only in trace amounts, and the prevalence of Aquificae might be connected with their ability to oxidize the ferrous iron present in CHS sediments. Armatimonadetes, Proteobacteria, Deinococcus-Thermus, Dictyoglomi, and Thermotogae, as well as uncultured bacteria (candidate divisions Oct-Spa1-106, GAL15, and OPB56), were numerous, and Cyanobacteria were present in low numbers. Archaea (less than 8% of the total community of each tested spring) belonged to Bathyarchaeota, Aigarchaeota, and Thaumarchaeota. The geographical location and the predominantly autotrophic microbial community, built on mechanisms other than the sulfur cycle-based ones, make CHS a special and unique terrestrial geothermal ecosystem. Text Arctic Chukotka permafrost PubMed Central (PMC) Arctic Microorganisms 8 9 1308 |
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Article Kochetkova, Tatiana V. Toshchakov, Stepan V. Zayulina, Kseniya S. Elcheninov, Alexander G. Zavarzina, Daria G. Lavrushin, Vasiliy Yu. Bonch-Osmolovskaya, Elizaveta A. Kublanov, Ilya V. Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
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description |
Chukotka is an arctic region located in the continuous permafrost zone, but thermal springs are abundant there. In this study, for the first time, the microbial communities of the Chukotka hot springs (CHS) biofilms and sediments with temperatures 54–94 °C were investigated and analyzed by NGS sequencing of 16S rRNA gene amplicons. In microbial mats (54–75 °C), phototrophic bacteria of genus Chloroflexus dominated (up to 89% of all prokaryotes), while Aquificae were the most numerous at higher temperatures in Fe-rich sediments and filamentous “streamers” (up to 92%). The electron donors typical for Aquificae, such as H(2)S and H(2), are absent or present only in trace amounts, and the prevalence of Aquificae might be connected with their ability to oxidize the ferrous iron present in CHS sediments. Armatimonadetes, Proteobacteria, Deinococcus-Thermus, Dictyoglomi, and Thermotogae, as well as uncultured bacteria (candidate divisions Oct-Spa1-106, GAL15, and OPB56), were numerous, and Cyanobacteria were present in low numbers. Archaea (less than 8% of the total community of each tested spring) belonged to Bathyarchaeota, Aigarchaeota, and Thaumarchaeota. The geographical location and the predominantly autotrophic microbial community, built on mechanisms other than the sulfur cycle-based ones, make CHS a special and unique terrestrial geothermal ecosystem. |
format |
Text |
author |
Kochetkova, Tatiana V. Toshchakov, Stepan V. Zayulina, Kseniya S. Elcheninov, Alexander G. Zavarzina, Daria G. Lavrushin, Vasiliy Yu. Bonch-Osmolovskaya, Elizaveta A. Kublanov, Ilya V. |
author_facet |
Kochetkova, Tatiana V. Toshchakov, Stepan V. Zayulina, Kseniya S. Elcheninov, Alexander G. Zavarzina, Daria G. Lavrushin, Vasiliy Yu. Bonch-Osmolovskaya, Elizaveta A. Kublanov, Ilya V. |
author_sort |
Kochetkova, Tatiana V. |
title |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_short |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_full |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_fullStr |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_full_unstemmed |
Hot in Cold: Microbial Life in the Hottest Springs in Permafrost |
title_sort |
hot in cold: microbial life in the hottest springs in permafrost |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565842/ http://www.ncbi.nlm.nih.gov/pubmed/32867302 https://doi.org/10.3390/microorganisms8091308 |
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Arctic |
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Arctic |
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Arctic Chukotka permafrost |
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Arctic Chukotka permafrost |
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Microorganisms |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565842/ http://www.ncbi.nlm.nih.gov/pubmed/32867302 http://dx.doi.org/10.3390/microorganisms8091308 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
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https://doi.org/10.3390/microorganisms8091308 |
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Microorganisms |
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1308 |
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