Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems
Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human inf...
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ftpubmed:oai:pubmedcentral.nih.gov:7565065 2023-05-15T14:00:43+02:00 Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems Blanco-Picazo, Pedro Roscales, Gabriel Toribio-Avedillo, Daniel Gómez-Gómez, Clara Avila, Conxita Ballesté, Elisenda Muniesa, Maite Rodríguez-Rubio, Lorena 2020-08-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565065/ http://www.ncbi.nlm.nih.gov/pubmed/32847015 https://doi.org/10.3390/microorganisms8091293 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565065/ http://www.ncbi.nlm.nih.gov/pubmed/32847015 http://dx.doi.org/10.3390/microorganisms8091293 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Microorganisms Article Text 2020 ftpubmed https://doi.org/10.3390/microorganisms8091293 2020-11-01T01:29:57Z Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human influence. Bacteriophages are known to play a relevant role in the dissemination of antibiotic resistance genes (ARGs) in aquatic environments. In this study, five ARGs (bla(TEM), bla(CTX-M-1), bla(CTX-M-9), sul1 and tetW) were monitored in phage particles isolated from seawater of two different locations: (i) the Mediterranean coast, subjected to high anthropogenic pressure, and (ii) the Antarctic coast, where the anthropogenic impact is low. Although found in lower quantities, ARG-containing phage particles were more prevalent among the Antarctic than the Mediterranean seawater samples and Antarctic bacterial communities were confirmed as their source. In the Mediterranean area, ARG-containing phages from anthropogenic fecal pollution might allow ARG transmission through the food chain. ARGs were detected in phage particles isolated from fish (Mediterranean, Atlantic, farmed, and frozen), the most abundant being β-lactamases. Some of these particles were infectious in cultures of the fecal bacteria Escherichia coli. By serving as ARG reservoirs in marine environments, including those with low human activity, such as the Antarctic, phages could contribute to ARG transmission between bacterial communities. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic Microorganisms 8 9 1293 |
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Article Blanco-Picazo, Pedro Roscales, Gabriel Toribio-Avedillo, Daniel Gómez-Gómez, Clara Avila, Conxita Ballesté, Elisenda Muniesa, Maite Rodríguez-Rubio, Lorena Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
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Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human influence. Bacteriophages are known to play a relevant role in the dissemination of antibiotic resistance genes (ARGs) in aquatic environments. In this study, five ARGs (bla(TEM), bla(CTX-M-1), bla(CTX-M-9), sul1 and tetW) were monitored in phage particles isolated from seawater of two different locations: (i) the Mediterranean coast, subjected to high anthropogenic pressure, and (ii) the Antarctic coast, where the anthropogenic impact is low. Although found in lower quantities, ARG-containing phage particles were more prevalent among the Antarctic than the Mediterranean seawater samples and Antarctic bacterial communities were confirmed as their source. In the Mediterranean area, ARG-containing phages from anthropogenic fecal pollution might allow ARG transmission through the food chain. ARGs were detected in phage particles isolated from fish (Mediterranean, Atlantic, farmed, and frozen), the most abundant being β-lactamases. Some of these particles were infectious in cultures of the fecal bacteria Escherichia coli. By serving as ARG reservoirs in marine environments, including those with low human activity, such as the Antarctic, phages could contribute to ARG transmission between bacterial communities. |
format |
Text |
author |
Blanco-Picazo, Pedro Roscales, Gabriel Toribio-Avedillo, Daniel Gómez-Gómez, Clara Avila, Conxita Ballesté, Elisenda Muniesa, Maite Rodríguez-Rubio, Lorena |
author_facet |
Blanco-Picazo, Pedro Roscales, Gabriel Toribio-Avedillo, Daniel Gómez-Gómez, Clara Avila, Conxita Ballesté, Elisenda Muniesa, Maite Rodríguez-Rubio, Lorena |
author_sort |
Blanco-Picazo, Pedro |
title |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_short |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_full |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_fullStr |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_full_unstemmed |
Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems |
title_sort |
antibiotic resistance genes in phage particles from antarctic and mediterranean seawater ecosystems |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565065/ http://www.ncbi.nlm.nih.gov/pubmed/32847015 https://doi.org/10.3390/microorganisms8091293 |
geographic |
Antarctic The Antarctic |
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Antarctic The Antarctic |
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Antarc* Antarctic |
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Antarc* Antarctic |
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Microorganisms |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7565065/ http://www.ncbi.nlm.nih.gov/pubmed/32847015 http://dx.doi.org/10.3390/microorganisms8091293 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/microorganisms8091293 |
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Microorganisms |
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8 |
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9 |
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1293 |
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1766270046606196736 |