Microbiota Modulates the Immunomodulatory Effects of Filifolinone on Atlantic Salmon
Filifolinone is an aromatic geranyl derivative, a natural compound isolated from Heliotropum sclerocarpum, which has immunomodulatory effects on Atlantic salmon, upregulating cytokines involved in Th1-type responses through a mechanism that remains unknown. In this work, we determined whether the im...
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ftpubmed:oai:pubmedcentral.nih.gov:7564783 2023-05-15T15:31:10+02:00 Microbiota Modulates the Immunomodulatory Effects of Filifolinone on Atlantic Salmon Parra, Mick Espinoza, Daniela Valdes, Natalia Vargas, Rodrigo Gonzalez, Alex Modak, Brenda Tello, Mario 2020-08-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564783/ http://www.ncbi.nlm.nih.gov/pubmed/32872599 https://doi.org/10.3390/microorganisms8091320 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564783/ http://www.ncbi.nlm.nih.gov/pubmed/32872599 http://dx.doi.org/10.3390/microorganisms8091320 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Microorganisms Article Text 2020 ftpubmed https://doi.org/10.3390/microorganisms8091320 2020-11-01T01:29:21Z Filifolinone is an aromatic geranyl derivative, a natural compound isolated from Heliotropum sclerocarpum, which has immunomodulatory effects on Atlantic salmon, upregulating cytokines involved in Th1-type responses through a mechanism that remains unknown. In this work, we determined whether the immunomodulatory effects of filifolinone depend on the host microbiotic composition. We evaluated the effect of filifolinone on immune genes and intestinal microbiotic composition of normal fish and fish previously treated with bacitracin/neomycin. Filifolinone induced the early expression of IFN-α1 and TGF-β, followed by the induction of TNF-α, IL-1β, and IFN-γ. A pre-treatment with antibiotics modified this effect, mainly changing the expression of IL-1β and IFN-γ. The evaluation of microbial diversity shows that filifolinone modifies the composition of intestinal microbiota, increasing the abundance of immunostimulating organisms like yeast and firmicutes. We identified 69 operational taxonomic units (OTUs) associated with filifolinone-induced IFN-γ. Our results indicate that filifolinone stimulates the immune system in two ways, one dependent on fish microbiota and the other not. To our knowledge, this is the first report of microbiota-dependent immunostimulation in Salmonids. Text Atlantic salmon PubMed Central (PMC) Microorganisms 8 9 1320 |
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Article Parra, Mick Espinoza, Daniela Valdes, Natalia Vargas, Rodrigo Gonzalez, Alex Modak, Brenda Tello, Mario Microbiota Modulates the Immunomodulatory Effects of Filifolinone on Atlantic Salmon |
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Filifolinone is an aromatic geranyl derivative, a natural compound isolated from Heliotropum sclerocarpum, which has immunomodulatory effects on Atlantic salmon, upregulating cytokines involved in Th1-type responses through a mechanism that remains unknown. In this work, we determined whether the immunomodulatory effects of filifolinone depend on the host microbiotic composition. We evaluated the effect of filifolinone on immune genes and intestinal microbiotic composition of normal fish and fish previously treated with bacitracin/neomycin. Filifolinone induced the early expression of IFN-α1 and TGF-β, followed by the induction of TNF-α, IL-1β, and IFN-γ. A pre-treatment with antibiotics modified this effect, mainly changing the expression of IL-1β and IFN-γ. The evaluation of microbial diversity shows that filifolinone modifies the composition of intestinal microbiota, increasing the abundance of immunostimulating organisms like yeast and firmicutes. We identified 69 operational taxonomic units (OTUs) associated with filifolinone-induced IFN-γ. Our results indicate that filifolinone stimulates the immune system in two ways, one dependent on fish microbiota and the other not. To our knowledge, this is the first report of microbiota-dependent immunostimulation in Salmonids. |
format |
Text |
author |
Parra, Mick Espinoza, Daniela Valdes, Natalia Vargas, Rodrigo Gonzalez, Alex Modak, Brenda Tello, Mario |
author_facet |
Parra, Mick Espinoza, Daniela Valdes, Natalia Vargas, Rodrigo Gonzalez, Alex Modak, Brenda Tello, Mario |
author_sort |
Parra, Mick |
title |
Microbiota Modulates the Immunomodulatory Effects of Filifolinone on Atlantic Salmon |
title_short |
Microbiota Modulates the Immunomodulatory Effects of Filifolinone on Atlantic Salmon |
title_full |
Microbiota Modulates the Immunomodulatory Effects of Filifolinone on Atlantic Salmon |
title_fullStr |
Microbiota Modulates the Immunomodulatory Effects of Filifolinone on Atlantic Salmon |
title_full_unstemmed |
Microbiota Modulates the Immunomodulatory Effects of Filifolinone on Atlantic Salmon |
title_sort |
microbiota modulates the immunomodulatory effects of filifolinone on atlantic salmon |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564783/ http://www.ncbi.nlm.nih.gov/pubmed/32872599 https://doi.org/10.3390/microorganisms8091320 |
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Atlantic salmon |
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Atlantic salmon |
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Microorganisms |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7564783/ http://www.ncbi.nlm.nih.gov/pubmed/32872599 http://dx.doi.org/10.3390/microorganisms8091320 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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https://doi.org/10.3390/microorganisms8091320 |
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Microorganisms |
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1320 |
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