Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat?
Transmission of pathogenic and resistant bacteria from wildlife to the bacterial gene pool in nature affects the ecosystem. Hence, we studied intestine content of five wild rodent species: the yellow-necked wood mouse (Apodemus flavicollis, n = 121), striped field mouse (Apodemus agrarius, n = 75),...
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ftpubmed:oai:pubmedcentral.nih.gov:7559071 2023-05-15T15:56:38+02:00 Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? Skarżyńska, Magdalena Zając, Magdalena Kamińska, Ewelina Bomba, Arkadiusz Żmudzki, Jacek Jabłoński, Artur Wasyl, Dariusz 2020-09-21 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559071/ http://www.ncbi.nlm.nih.gov/pubmed/32967245 https://doi.org/10.3390/pathogens9090771 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559071/ http://www.ncbi.nlm.nih.gov/pubmed/32967245 http://dx.doi.org/10.3390/pathogens9090771 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Pathogens Article Text 2020 ftpubmed https://doi.org/10.3390/pathogens9090771 2020-11-01T01:22:43Z Transmission of pathogenic and resistant bacteria from wildlife to the bacterial gene pool in nature affects the ecosystem. Hence, we studied intestine content of five wild rodent species: the yellow-necked wood mouse (Apodemus flavicollis, n = 121), striped field mouse (Apodemus agrarius, n = 75), common vole (Microtus arvalis, n = 37), bank vole (Myodes glareolus, n = 3), and house mouse (Mus musculus, n = 1) to assess their potential role as an antimicrobial resistance (AMR) and Salmonella vector. The methods adopted from official AMR monitoring of slaughtered animals were applied and supplemented with colistin resistance screening. Whole-genome sequencing of obtained bacteria elucidated their epidemiological relationships and zoonotic potential. The study revealed no indications of public health relevance of wild rodents from the sampled area in Salmonella spread and their limited role in AMR dissemination. Of 263 recovered E. coli, the vast majority was pan-susceptible, and as few as 5 E. coli showed any resistance. In four colistin-resistant strains neither the known mcr genes nor known mutations in pmr genes were found. One of these strains was tetracycline-resistant due to tet(B). High diversity of virulence factors (n = 43) noted in tested strains including ibeA, cdtB, air, eilA, astA, vat, pic reported in clinically relevant types of enteric E. coli indicate that rodents may be involved in the ecological cycle of these bacteria. Most of the strains represented unique sequence types and ST10805, ST10806, ST10810, ST10824 were revealed for the first time, showing genomic heterogeneity of the strains. The study broadened the knowledge on phylogenetic diversity and structure of the E. coli population in wild rodents. Text Common vole Microtus arvalis PubMed Central (PMC) Pathogens 9 9 771 |
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Article Skarżyńska, Magdalena Zając, Magdalena Kamińska, Ewelina Bomba, Arkadiusz Żmudzki, Jacek Jabłoński, Artur Wasyl, Dariusz Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
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Transmission of pathogenic and resistant bacteria from wildlife to the bacterial gene pool in nature affects the ecosystem. Hence, we studied intestine content of five wild rodent species: the yellow-necked wood mouse (Apodemus flavicollis, n = 121), striped field mouse (Apodemus agrarius, n = 75), common vole (Microtus arvalis, n = 37), bank vole (Myodes glareolus, n = 3), and house mouse (Mus musculus, n = 1) to assess their potential role as an antimicrobial resistance (AMR) and Salmonella vector. The methods adopted from official AMR monitoring of slaughtered animals were applied and supplemented with colistin resistance screening. Whole-genome sequencing of obtained bacteria elucidated their epidemiological relationships and zoonotic potential. The study revealed no indications of public health relevance of wild rodents from the sampled area in Salmonella spread and their limited role in AMR dissemination. Of 263 recovered E. coli, the vast majority was pan-susceptible, and as few as 5 E. coli showed any resistance. In four colistin-resistant strains neither the known mcr genes nor known mutations in pmr genes were found. One of these strains was tetracycline-resistant due to tet(B). High diversity of virulence factors (n = 43) noted in tested strains including ibeA, cdtB, air, eilA, astA, vat, pic reported in clinically relevant types of enteric E. coli indicate that rodents may be involved in the ecological cycle of these bacteria. Most of the strains represented unique sequence types and ST10805, ST10806, ST10810, ST10824 were revealed for the first time, showing genomic heterogeneity of the strains. The study broadened the knowledge on phylogenetic diversity and structure of the E. coli population in wild rodents. |
format |
Text |
author |
Skarżyńska, Magdalena Zając, Magdalena Kamińska, Ewelina Bomba, Arkadiusz Żmudzki, Jacek Jabłoński, Artur Wasyl, Dariusz |
author_facet |
Skarżyńska, Magdalena Zając, Magdalena Kamińska, Ewelina Bomba, Arkadiusz Żmudzki, Jacek Jabłoński, Artur Wasyl, Dariusz |
author_sort |
Skarżyńska, Magdalena |
title |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_short |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_full |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_fullStr |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_full_unstemmed |
Salmonella and Antimicrobial Resistance in Wild Rodents—True or False Threat? |
title_sort |
salmonella and antimicrobial resistance in wild rodents—true or false threat? |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559071/ http://www.ncbi.nlm.nih.gov/pubmed/32967245 https://doi.org/10.3390/pathogens9090771 |
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Common vole Microtus arvalis |
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Common vole Microtus arvalis |
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Pathogens |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7559071/ http://www.ncbi.nlm.nih.gov/pubmed/32967245 http://dx.doi.org/10.3390/pathogens9090771 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/pathogens9090771 |
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Pathogens |
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