Fermented Oyster Extract Promotes Insulin-Like Growth Factor-1-Mediated Osteogenesis and Growth Rate
Fermented oyster (Crassostrea gigas) extract (FO) prevents ovariectomy-induced osteoporosis by inhibiting osteoclastogenesis and activating osteogenesis. However, the molecular mechanisms underlying FO-mediated bone formation and growth rate are unclear. In the current study, we found that FO signif...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551862/ http://www.ncbi.nlm.nih.gov/pubmed/32962034 https://doi.org/10.3390/md18090472 |
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ftpubmed:oai:pubmedcentral.nih.gov:7551862 2023-05-15T15:59:05+02:00 Fermented Oyster Extract Promotes Insulin-Like Growth Factor-1-Mediated Osteogenesis and Growth Rate Molagoda, Ilandarage Menu Neelaka Jayasingha, Jayasingha Arachchige Chathuranga Chanaka Choi, Yung Hyun Park, Eui Kyun Jeon, You-Jin Lee, Bae-Jin Kim, Gi-Young 2020-09-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551862/ http://www.ncbi.nlm.nih.gov/pubmed/32962034 https://doi.org/10.3390/md18090472 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551862/ http://www.ncbi.nlm.nih.gov/pubmed/32962034 http://dx.doi.org/10.3390/md18090472 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Mar Drugs Article Text 2020 ftpubmed https://doi.org/10.3390/md18090472 2020-10-18T00:37:54Z Fermented oyster (Crassostrea gigas) extract (FO) prevents ovariectomy-induced osteoporosis by inhibiting osteoclastogenesis and activating osteogenesis. However, the molecular mechanisms underlying FO-mediated bone formation and growth rate are unclear. In the current study, we found that FO significantly upregulated the expression of growth-promoting genes in zebrafish larvae including insulin-like growth factor 1 (zigf-1), insulin-like growth factor binding protein 3 (zigfbp-3), growth hormone-1 (zgh-1), growth hormone receptor-1 (zghr-1), growth hormone receptor alpha (zghra), glucokinase (zgck), and cholecystokinin (zccka). In addition, zebrafish larvae treated with 100 μg/mL FO increased in total body length (3.89 ± 0.13 mm) at 12 days post fertilization (dpf) compared to untreated larvae (3.69 ± 0.02 mm); this effect was comparable to that of the β-glycerophosphate-treated zebrafish larvae (4.00 ± 0.02 mm). Furthermore, FO time- and dose-dependently increased the extracellular release of IGF-1 from preosteoblast MC3T3-E1 cells, which was accompanied by high expression of IGF-1. Pharmacological inhibition of IGF-1 receptor (IGF-1R) using picropodophyllin (PPP) significantly reduced FO-mediated vertebrae formation (from 9.19 ± 0.31 to 5.53 ± 0.35) and growth performance (from 3.91 ± 0.02 to 3.69 ± 0.01 mm) in zebrafish larvae at 9 dpf. Similarly, PPP significantly decreased FO-induced calcium deposition in MC3T3-E1 cells by inhibiting GSK-3β phosphorylation at Ser9. Additionally, DOI hydrochloride, a potent stabilizer of GSK-3β, reduced FO-induced nuclear translocation of RUNX2. Transient knockdown of IGF-1Rα/β using specific silencing RNA also resulted in a significant decrease in calcium deposition and reduction in GSK-3β phosphorylation at Ser9 in MC3T3-E1 cells. Altogether, these results indicate that FO increased phosphorylated GSK-3β at Ser9 by activating the autocrine IGF-1-mediated IGF-1R signaling pathway, thereby promoting osteogenesis and growth performance. Therefore, FO is a potential ... Text Crassostrea gigas PubMed Central (PMC) Marine Drugs 18 9 472 |
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Article Molagoda, Ilandarage Menu Neelaka Jayasingha, Jayasingha Arachchige Chathuranga Chanaka Choi, Yung Hyun Park, Eui Kyun Jeon, You-Jin Lee, Bae-Jin Kim, Gi-Young Fermented Oyster Extract Promotes Insulin-Like Growth Factor-1-Mediated Osteogenesis and Growth Rate |
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Article |
description |
Fermented oyster (Crassostrea gigas) extract (FO) prevents ovariectomy-induced osteoporosis by inhibiting osteoclastogenesis and activating osteogenesis. However, the molecular mechanisms underlying FO-mediated bone formation and growth rate are unclear. In the current study, we found that FO significantly upregulated the expression of growth-promoting genes in zebrafish larvae including insulin-like growth factor 1 (zigf-1), insulin-like growth factor binding protein 3 (zigfbp-3), growth hormone-1 (zgh-1), growth hormone receptor-1 (zghr-1), growth hormone receptor alpha (zghra), glucokinase (zgck), and cholecystokinin (zccka). In addition, zebrafish larvae treated with 100 μg/mL FO increased in total body length (3.89 ± 0.13 mm) at 12 days post fertilization (dpf) compared to untreated larvae (3.69 ± 0.02 mm); this effect was comparable to that of the β-glycerophosphate-treated zebrafish larvae (4.00 ± 0.02 mm). Furthermore, FO time- and dose-dependently increased the extracellular release of IGF-1 from preosteoblast MC3T3-E1 cells, which was accompanied by high expression of IGF-1. Pharmacological inhibition of IGF-1 receptor (IGF-1R) using picropodophyllin (PPP) significantly reduced FO-mediated vertebrae formation (from 9.19 ± 0.31 to 5.53 ± 0.35) and growth performance (from 3.91 ± 0.02 to 3.69 ± 0.01 mm) in zebrafish larvae at 9 dpf. Similarly, PPP significantly decreased FO-induced calcium deposition in MC3T3-E1 cells by inhibiting GSK-3β phosphorylation at Ser9. Additionally, DOI hydrochloride, a potent stabilizer of GSK-3β, reduced FO-induced nuclear translocation of RUNX2. Transient knockdown of IGF-1Rα/β using specific silencing RNA also resulted in a significant decrease in calcium deposition and reduction in GSK-3β phosphorylation at Ser9 in MC3T3-E1 cells. Altogether, these results indicate that FO increased phosphorylated GSK-3β at Ser9 by activating the autocrine IGF-1-mediated IGF-1R signaling pathway, thereby promoting osteogenesis and growth performance. Therefore, FO is a potential ... |
format |
Text |
author |
Molagoda, Ilandarage Menu Neelaka Jayasingha, Jayasingha Arachchige Chathuranga Chanaka Choi, Yung Hyun Park, Eui Kyun Jeon, You-Jin Lee, Bae-Jin Kim, Gi-Young |
author_facet |
Molagoda, Ilandarage Menu Neelaka Jayasingha, Jayasingha Arachchige Chathuranga Chanaka Choi, Yung Hyun Park, Eui Kyun Jeon, You-Jin Lee, Bae-Jin Kim, Gi-Young |
author_sort |
Molagoda, Ilandarage Menu Neelaka |
title |
Fermented Oyster Extract Promotes Insulin-Like Growth Factor-1-Mediated Osteogenesis and Growth Rate |
title_short |
Fermented Oyster Extract Promotes Insulin-Like Growth Factor-1-Mediated Osteogenesis and Growth Rate |
title_full |
Fermented Oyster Extract Promotes Insulin-Like Growth Factor-1-Mediated Osteogenesis and Growth Rate |
title_fullStr |
Fermented Oyster Extract Promotes Insulin-Like Growth Factor-1-Mediated Osteogenesis and Growth Rate |
title_full_unstemmed |
Fermented Oyster Extract Promotes Insulin-Like Growth Factor-1-Mediated Osteogenesis and Growth Rate |
title_sort |
fermented oyster extract promotes insulin-like growth factor-1-mediated osteogenesis and growth rate |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551862/ http://www.ncbi.nlm.nih.gov/pubmed/32962034 https://doi.org/10.3390/md18090472 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551862/ http://www.ncbi.nlm.nih.gov/pubmed/32962034 http://dx.doi.org/10.3390/md18090472 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/md18090472 |
container_title |
Marine Drugs |
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18 |
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9 |
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472 |
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