A Novel Cold-adapted Methylovulum species, with a High C16:1ω5c Content, Isolated from an Arctic Thermal Spring in Spitsbergen
A novel cold-adapted methane-oxidizing bacterium, termed TFB, was isolated from the thermoglacial Arctic karst spring, Trollosen, located in the South Spitsbergen National Park (Norway). The source water is cold and extremely low in phosphate and nitrate. The isolate belongs to the Methylovulum genu...
Published in: | Microbes and Environments |
---|---|
Main Authors: | , , |
Format: | Text |
Language: | English |
Published: |
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
2020
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511782/ http://www.ncbi.nlm.nih.gov/pubmed/32536671 https://doi.org/10.1264/jsme2.ME20044 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:7511782 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:7511782 2023-05-15T14:52:36+02:00 A Novel Cold-adapted Methylovulum species, with a High C16:1ω5c Content, Isolated from an Arctic Thermal Spring in Spitsbergen Islam, Tajul Larsen, Øivind Birkeland, Nils-Kåre 2020 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511782/ http://www.ncbi.nlm.nih.gov/pubmed/32536671 https://doi.org/10.1264/jsme2.ME20044 en eng Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511782/ http://www.ncbi.nlm.nih.gov/pubmed/32536671 http://dx.doi.org/10.1264/jsme2.ME20044 2020 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Microbes Environ Regular Paper Text 2020 ftpubmed https://doi.org/10.1264/jsme2.ME20044 2020-11-22T01:18:39Z A novel cold-adapted methane-oxidizing bacterium, termed TFB, was isolated from the thermoglacial Arctic karst spring, Trollosen, located in the South Spitsbergen National Park (Norway). The source water is cold and extremely low in phosphate and nitrate. The isolate belongs to the Methylovulum genus of gammaproteobacterial methanotrophs, with the closest phylogenetic affiliation with Methylovulum miyakonense and Methylovulum psychrotolerans (96.2 and 96.1% 16S rRNA gene sequence similarities, respectively). TFB is a strict aerobe that only grows in the presence of methane or methanol. It fixes atmospheric nitrogen and contains Type I intracellular membranes. The growth temperature range was 2–22°C, with an optimum at 13–18°C. The functional genes pmoA, mxaF, and nifH were identified by PCR, whereas mmoX and cbbL were not. C16:1ω5c was identified as the major fatty acid constituent, at an amount (>49%) not previously found in any methanotrophs, and is likely to play a major role in cold adaptation. Strain TFB may be regarded as a new psychrotolerant or psychrophilic species within the genus Methylovulum. The recovery of this cold-adapted bacterium from a neutral Arctic thermal spring increases our knowledge of the diversity and adaptation of extremophilic gammaproteobacterial methanotrophs in the candidate family “Methylomonadaceae”. Text Arctic Spitsbergen PubMed Central (PMC) Arctic Norway Trollosen ENVELOPE(15.376,15.376,68.018,68.018) Microbes and Environments 35 3 n/a |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Regular Paper |
spellingShingle |
Regular Paper Islam, Tajul Larsen, Øivind Birkeland, Nils-Kåre A Novel Cold-adapted Methylovulum species, with a High C16:1ω5c Content, Isolated from an Arctic Thermal Spring in Spitsbergen |
topic_facet |
Regular Paper |
description |
A novel cold-adapted methane-oxidizing bacterium, termed TFB, was isolated from the thermoglacial Arctic karst spring, Trollosen, located in the South Spitsbergen National Park (Norway). The source water is cold and extremely low in phosphate and nitrate. The isolate belongs to the Methylovulum genus of gammaproteobacterial methanotrophs, with the closest phylogenetic affiliation with Methylovulum miyakonense and Methylovulum psychrotolerans (96.2 and 96.1% 16S rRNA gene sequence similarities, respectively). TFB is a strict aerobe that only grows in the presence of methane or methanol. It fixes atmospheric nitrogen and contains Type I intracellular membranes. The growth temperature range was 2–22°C, with an optimum at 13–18°C. The functional genes pmoA, mxaF, and nifH were identified by PCR, whereas mmoX and cbbL were not. C16:1ω5c was identified as the major fatty acid constituent, at an amount (>49%) not previously found in any methanotrophs, and is likely to play a major role in cold adaptation. Strain TFB may be regarded as a new psychrotolerant or psychrophilic species within the genus Methylovulum. The recovery of this cold-adapted bacterium from a neutral Arctic thermal spring increases our knowledge of the diversity and adaptation of extremophilic gammaproteobacterial methanotrophs in the candidate family “Methylomonadaceae”. |
format |
Text |
author |
Islam, Tajul Larsen, Øivind Birkeland, Nils-Kåre |
author_facet |
Islam, Tajul Larsen, Øivind Birkeland, Nils-Kåre |
author_sort |
Islam, Tajul |
title |
A Novel Cold-adapted Methylovulum species, with a High C16:1ω5c Content, Isolated from an Arctic Thermal Spring in Spitsbergen |
title_short |
A Novel Cold-adapted Methylovulum species, with a High C16:1ω5c Content, Isolated from an Arctic Thermal Spring in Spitsbergen |
title_full |
A Novel Cold-adapted Methylovulum species, with a High C16:1ω5c Content, Isolated from an Arctic Thermal Spring in Spitsbergen |
title_fullStr |
A Novel Cold-adapted Methylovulum species, with a High C16:1ω5c Content, Isolated from an Arctic Thermal Spring in Spitsbergen |
title_full_unstemmed |
A Novel Cold-adapted Methylovulum species, with a High C16:1ω5c Content, Isolated from an Arctic Thermal Spring in Spitsbergen |
title_sort |
novel cold-adapted methylovulum species, with a high c16:1ω5c content, isolated from an arctic thermal spring in spitsbergen |
publisher |
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511782/ http://www.ncbi.nlm.nih.gov/pubmed/32536671 https://doi.org/10.1264/jsme2.ME20044 |
long_lat |
ENVELOPE(15.376,15.376,68.018,68.018) |
geographic |
Arctic Norway Trollosen |
geographic_facet |
Arctic Norway Trollosen |
genre |
Arctic Spitsbergen |
genre_facet |
Arctic Spitsbergen |
op_source |
Microbes Environ |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7511782/ http://www.ncbi.nlm.nih.gov/pubmed/32536671 http://dx.doi.org/10.1264/jsme2.ME20044 |
op_rights |
2020 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1264/jsme2.ME20044 |
container_title |
Microbes and Environments |
container_volume |
35 |
container_issue |
3 |
container_start_page |
n/a |
_version_ |
1766323827908804608 |