Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the...
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ftpubmed:oai:pubmedcentral.nih.gov:7503493 2023-05-15T13:42:08+02:00 Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica Ibrahim, Salihu Abdul Khalil, Khalilah Zahri, Khadijah Nabilah Mohd Gomez-Fuentes, Claudio Convey, Peter Zulkharnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima 2020-08-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503493/ https://doi.org/10.3390/molecules25173878 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503493/ http://dx.doi.org/10.3390/molecules25173878 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Molecules Article Text 2020 ftpubmed https://doi.org/10.3390/molecules25173878 2020-09-27T00:37:29Z With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (q(max)), concentration of substrate at the half maximum specific growth rate, K(s)% (v/v) and the inhibition constant K(i)% (v/v), with values of 0.142 h(−1), 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic The Antarctic Molecules 25 17 3878 |
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Article Ibrahim, Salihu Abdul Khalil, Khalilah Zahri, Khadijah Nabilah Mohd Gomez-Fuentes, Claudio Convey, Peter Zulkharnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
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Article |
description |
With the progressive increase in human activities in the Antarctic region, the possibility of domestic oil spillage also increases. Developing means for the removal of oils, such as canola oil, from the environment and waste “grey” water using biological approaches is therefore desirable, since the thermal process of oil degradation is expensive and ineffective. Thus, in this study an indigenous cold-adapted Antarctic soil bacterium, Rhodococcus erythropolis strain AQ5-07, was screened for biosurfactant production ability using the multiple approaches of blood haemolysis, surface tension, emulsification index, oil spreading, drop collapse and “MATH” assay for cellular hydrophobicity. The growth kinetics of the bacterium containing different canola oil concentration was studied. The strain showed β-haemolysis on blood agar with a high emulsification index and low surface tension value of 91.5% and 25.14 mN/m, respectively. Of the models tested, the Haldane model provided the best description of the growth kinetics, although several models were similar in performance. Parameters obtained from the modelling were the maximum specific growth rate (q(max)), concentration of substrate at the half maximum specific growth rate, K(s)% (v/v) and the inhibition constant K(i)% (v/v), with values of 0.142 h(−1), 7.743% (v/v) and 0.399% (v/v), respectively. These biological coefficients are useful in predicting growth conditions for batch studies, and also relevant to “in field” bioremediation strategies where the concentration of oil might need to be diluted to non-toxic levels prior to remediation. Biosurfactants can also have application in enhanced oil recovery (EOR) under different environmental conditions. |
format |
Text |
author |
Ibrahim, Salihu Abdul Khalil, Khalilah Zahri, Khadijah Nabilah Mohd Gomez-Fuentes, Claudio Convey, Peter Zulkharnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima |
author_facet |
Ibrahim, Salihu Abdul Khalil, Khalilah Zahri, Khadijah Nabilah Mohd Gomez-Fuentes, Claudio Convey, Peter Zulkharnain, Azham Sabri, Suriana Alias, Siti Aisyah González-Rocha, Gerardo Ahmad, Siti Aqlima |
author_sort |
Ibrahim, Salihu |
title |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_short |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_full |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_fullStr |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_full_unstemmed |
Biosurfactant Production and Growth Kinetics Studies of the Waste Canola Oil-Degrading Bacterium Rhodococcus erythropolis AQ5-07 from Antarctica |
title_sort |
biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium rhodococcus erythropolis aq5-07 from antarctica |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503493/ https://doi.org/10.3390/molecules25173878 |
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Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
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Antarc* Antarctic Antarctica |
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Antarc* Antarctic Antarctica |
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Molecules |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7503493/ http://dx.doi.org/10.3390/molecules25173878 |
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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
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https://doi.org/10.3390/molecules25173878 |
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Molecules |
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