Trogocytosis with monocytes associated with increased α2,3 sialic acid expression on B cells during H5N1 influenza virus infection

• Published in: PLOS ONE
• Main Authors: Kongsomros, Supasek, Thanunchai, Maytawan, Manopwisedjaroen, Suwimon, Na-Ek, Prasit, Wang, Sheng-Fan, Taechalertpaisarn, Tana, Thitithanyanont, Arunee
• Format: Text
• Language: English
• Published: Public Library of Science 2020
• Subjects: Research Article; Avian flu
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500609/; https://doi.org/10.1371/journal.pone.0239488

The immunopathogenesis of H5N1 virus has been studied intensively since it caused cross-species infection and induced high mortality to human. We previously observed the interaction between monocytes and B cells, which increased the susceptibility of B cell to H5N1 virus infection after a co-culture. Levels of α2,3 sialic acid (avian flu receptor) were also significantly increased on B cell surface in this co-culture model with unclear explanation. In this study, we aimed to determine the possible mechanism that responded for this increase in α2,3 sialic acid on B cells. Acquisition of α2,3 SA by B cells via cell contact-dependent trogocytosis was proposed. Results showed that the lack of α2,3 SA was detected on B cell surface, and B cells acquired membrane-bound α2,3 SA molecules from monocytes in H5N1-infected co-cultures. Occurrence of membrane exchange mainly relied on H5N1 infection and cell-cell contact as opposed to a mock infection and transwell. The increase in α2,3 SA on B cell surface mediated by trogocytosis was associated with the enhanced susceptibility to H5N1 infection. These observations thus provide the evidence that H5N1 influenza virus may utilize trogocytosis to expand its cell tropism and spread to immune cells despite the lack of avian flu receptor.