Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity
To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Epte...
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ftpubmed:oai:pubmedcentral.nih.gov:7472731 2023-05-15T17:13:46+02:00 Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity Šimić, Ivana Zorec, Tomaž Mark Lojkić, Ivana Krešić, Nina Poljak, Mario Cliquet, Florence Picard-Meyer, Evelyne Wasniewski, Marine Zrnčić, Vida Ćukušić, Anđela Bedeković, Tomislav 2020-08-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472731/ http://www.ncbi.nlm.nih.gov/pubmed/32824037 https://doi.org/10.3390/v12080891 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472731/ http://www.ncbi.nlm.nih.gov/pubmed/32824037 http://dx.doi.org/10.3390/v12080891 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Viruses Article Text 2020 ftpubmed https://doi.org/10.3390/v12080891 2020-09-20T00:25:27Z To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Eptesicus serotinus, Myotis blythii, Myotis nattereri and Myotis emarginatus) conducted in Mediterranean and continental Croatia. Viral nucleic acids were extracted from sample pools, and analyzed using Illumina sequencing. The presence of 63 different viral families representing all seven Baltimore groups were confirmed, most commonly insect viruses likely reflecting the diet of insectivorous bats. Virome compositions of our samples were largely impacted by the sample type: invertebrate-infecting viruses were most frequently found in feces, bacterial viruses in guano, whereas vertebrate-infecting viruses were most common in swabs. Most vertebrate-infecting virus sequences were assigned to retroviruses, parvoviruses, iridoviruses, and poxviruses. We further report the complete genome sequence of a novel adeno-associated virus, densovirus and a near complete length genome sequence of a novel iflavirus. Additionally, one of the most interesting findings in this study was the difference in viromes between two contrasting habitats, the continental and Mediterranean Croatia. Text Myotis nattereri PubMed Central (PMC) Guano ENVELOPE(141.604,141.604,-66.775,-66.775) Viruses 12 8 891 |
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Article Šimić, Ivana Zorec, Tomaž Mark Lojkić, Ivana Krešić, Nina Poljak, Mario Cliquet, Florence Picard-Meyer, Evelyne Wasniewski, Marine Zrnčić, Vida Ćukušić, Anđela Bedeković, Tomislav Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
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description |
To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Eptesicus serotinus, Myotis blythii, Myotis nattereri and Myotis emarginatus) conducted in Mediterranean and continental Croatia. Viral nucleic acids were extracted from sample pools, and analyzed using Illumina sequencing. The presence of 63 different viral families representing all seven Baltimore groups were confirmed, most commonly insect viruses likely reflecting the diet of insectivorous bats. Virome compositions of our samples were largely impacted by the sample type: invertebrate-infecting viruses were most frequently found in feces, bacterial viruses in guano, whereas vertebrate-infecting viruses were most common in swabs. Most vertebrate-infecting virus sequences were assigned to retroviruses, parvoviruses, iridoviruses, and poxviruses. We further report the complete genome sequence of a novel adeno-associated virus, densovirus and a near complete length genome sequence of a novel iflavirus. Additionally, one of the most interesting findings in this study was the difference in viromes between two contrasting habitats, the continental and Mediterranean Croatia. |
format |
Text |
author |
Šimić, Ivana Zorec, Tomaž Mark Lojkić, Ivana Krešić, Nina Poljak, Mario Cliquet, Florence Picard-Meyer, Evelyne Wasniewski, Marine Zrnčić, Vida Ćukušić, Anđela Bedeković, Tomislav |
author_facet |
Šimić, Ivana Zorec, Tomaž Mark Lojkić, Ivana Krešić, Nina Poljak, Mario Cliquet, Florence Picard-Meyer, Evelyne Wasniewski, Marine Zrnčić, Vida Ćukušić, Anđela Bedeković, Tomislav |
author_sort |
Šimić, Ivana |
title |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_short |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_full |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_fullStr |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_full_unstemmed |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_sort |
viral metagenomic profiling of croatian bat population reveals sample and habitat dependent diversity |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472731/ http://www.ncbi.nlm.nih.gov/pubmed/32824037 https://doi.org/10.3390/v12080891 |
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ENVELOPE(141.604,141.604,-66.775,-66.775) |
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Guano |
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Guano |
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Myotis nattereri |
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Myotis nattereri |
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Viruses |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472731/ http://www.ncbi.nlm.nih.gov/pubmed/32824037 http://dx.doi.org/10.3390/v12080891 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/v12080891 |
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Viruses |
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891 |
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