Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics

The isolation of soil bacteria from extreme environments represents a major challenge, but also an opportunity to characterize the metabolic potential of soil bacteria that could promote the growth of plants inhabiting these harsh conditions. The aim of this study was to isolate and identify bacteri...

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Published in:Microorganisms
Main Authors: Gaete, Alexis, Mandakovic, Dinka, González, Mauricio
Format: Text
Language:English
Published: MDPI 2020
Subjects:
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466141/
http://www.ncbi.nlm.nih.gov/pubmed/32785053
https://doi.org/10.3390/microorganisms8081213
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spelling ftpubmed:oai:pubmedcentral.nih.gov:7466141 2023-05-15T13:52:06+02:00 Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics Gaete, Alexis Mandakovic, Dinka González, Mauricio 2020-08-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466141/ http://www.ncbi.nlm.nih.gov/pubmed/32785053 https://doi.org/10.3390/microorganisms8081213 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466141/ http://www.ncbi.nlm.nih.gov/pubmed/32785053 http://dx.doi.org/10.3390/microorganisms8081213 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Microorganisms Article Text 2020 ftpubmed https://doi.org/10.3390/microorganisms8081213 2020-09-20T00:20:20Z The isolation of soil bacteria from extreme environments represents a major challenge, but also an opportunity to characterize the metabolic potential of soil bacteria that could promote the growth of plants inhabiting these harsh conditions. The aim of this study was to isolate and identify bacteria from two Chilean desert environments and characterize the beneficial traits for plants through a biochemical approach. By means of different culture strategies, we obtained 39 bacterial soil isolates from the Coppermine Peninsula (Antarctica) and 32 from Lejía Lake shore soil (Atacama Desert). The results obtained from the taxonomic classification and phylogenetic analysis based on 16S rDNA sequences indicated that the isolates belonged to four phyla (Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes), and that the most represented genus at both sites was Pseudomonas. Regarding biochemical characterization, all strains displayed in vitro PGP capabilities, but these were in different proportions that grouped them according to their site of origin. This study contributes with microbial isolates from natural extreme environments with biotechnological potentials in improving plant growth under cold stress. Text Antarc* Antarctica PubMed Central (PMC) Coppermine Peninsula ENVELOPE(-59.713,-59.713,-62.374,-62.374) Microorganisms 8 8 1213
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Article
spellingShingle Article
Gaete, Alexis
Mandakovic, Dinka
González, Mauricio
Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics
topic_facet Article
description The isolation of soil bacteria from extreme environments represents a major challenge, but also an opportunity to characterize the metabolic potential of soil bacteria that could promote the growth of plants inhabiting these harsh conditions. The aim of this study was to isolate and identify bacteria from two Chilean desert environments and characterize the beneficial traits for plants through a biochemical approach. By means of different culture strategies, we obtained 39 bacterial soil isolates from the Coppermine Peninsula (Antarctica) and 32 from Lejía Lake shore soil (Atacama Desert). The results obtained from the taxonomic classification and phylogenetic analysis based on 16S rDNA sequences indicated that the isolates belonged to four phyla (Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes), and that the most represented genus at both sites was Pseudomonas. Regarding biochemical characterization, all strains displayed in vitro PGP capabilities, but these were in different proportions that grouped them according to their site of origin. This study contributes with microbial isolates from natural extreme environments with biotechnological potentials in improving plant growth under cold stress.
format Text
author Gaete, Alexis
Mandakovic, Dinka
González, Mauricio
author_facet Gaete, Alexis
Mandakovic, Dinka
González, Mauricio
author_sort Gaete, Alexis
title Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics
title_short Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics
title_full Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics
title_fullStr Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics
title_full_unstemmed Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics
title_sort isolation and identification of soil bacteria from extreme environments of chile and their plant beneficial characteristics
publisher MDPI
publishDate 2020
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466141/
http://www.ncbi.nlm.nih.gov/pubmed/32785053
https://doi.org/10.3390/microorganisms8081213
long_lat ENVELOPE(-59.713,-59.713,-62.374,-62.374)
geographic Coppermine Peninsula
geographic_facet Coppermine Peninsula
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Microorganisms
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466141/
http://www.ncbi.nlm.nih.gov/pubmed/32785053
http://dx.doi.org/10.3390/microorganisms8081213
op_rights © 2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
op_rightsnorm CC-BY
op_doi https://doi.org/10.3390/microorganisms8081213
container_title Microorganisms
container_volume 8
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