Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics
The isolation of soil bacteria from extreme environments represents a major challenge, but also an opportunity to characterize the metabolic potential of soil bacteria that could promote the growth of plants inhabiting these harsh conditions. The aim of this study was to isolate and identify bacteri...
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ftpubmed:oai:pubmedcentral.nih.gov:7466141 2023-05-15T13:52:06+02:00 Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics Gaete, Alexis Mandakovic, Dinka González, Mauricio 2020-08-10 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466141/ http://www.ncbi.nlm.nih.gov/pubmed/32785053 https://doi.org/10.3390/microorganisms8081213 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466141/ http://www.ncbi.nlm.nih.gov/pubmed/32785053 http://dx.doi.org/10.3390/microorganisms8081213 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Microorganisms Article Text 2020 ftpubmed https://doi.org/10.3390/microorganisms8081213 2020-09-20T00:20:20Z The isolation of soil bacteria from extreme environments represents a major challenge, but also an opportunity to characterize the metabolic potential of soil bacteria that could promote the growth of plants inhabiting these harsh conditions. The aim of this study was to isolate and identify bacteria from two Chilean desert environments and characterize the beneficial traits for plants through a biochemical approach. By means of different culture strategies, we obtained 39 bacterial soil isolates from the Coppermine Peninsula (Antarctica) and 32 from Lejía Lake shore soil (Atacama Desert). The results obtained from the taxonomic classification and phylogenetic analysis based on 16S rDNA sequences indicated that the isolates belonged to four phyla (Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes), and that the most represented genus at both sites was Pseudomonas. Regarding biochemical characterization, all strains displayed in vitro PGP capabilities, but these were in different proportions that grouped them according to their site of origin. This study contributes with microbial isolates from natural extreme environments with biotechnological potentials in improving plant growth under cold stress. Text Antarc* Antarctica PubMed Central (PMC) Coppermine Peninsula ENVELOPE(-59.713,-59.713,-62.374,-62.374) Microorganisms 8 8 1213 |
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Article Gaete, Alexis Mandakovic, Dinka González, Mauricio Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics |
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description |
The isolation of soil bacteria from extreme environments represents a major challenge, but also an opportunity to characterize the metabolic potential of soil bacteria that could promote the growth of plants inhabiting these harsh conditions. The aim of this study was to isolate and identify bacteria from two Chilean desert environments and characterize the beneficial traits for plants through a biochemical approach. By means of different culture strategies, we obtained 39 bacterial soil isolates from the Coppermine Peninsula (Antarctica) and 32 from Lejía Lake shore soil (Atacama Desert). The results obtained from the taxonomic classification and phylogenetic analysis based on 16S rDNA sequences indicated that the isolates belonged to four phyla (Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes), and that the most represented genus at both sites was Pseudomonas. Regarding biochemical characterization, all strains displayed in vitro PGP capabilities, but these were in different proportions that grouped them according to their site of origin. This study contributes with microbial isolates from natural extreme environments with biotechnological potentials in improving plant growth under cold stress. |
format |
Text |
author |
Gaete, Alexis Mandakovic, Dinka González, Mauricio |
author_facet |
Gaete, Alexis Mandakovic, Dinka González, Mauricio |
author_sort |
Gaete, Alexis |
title |
Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics |
title_short |
Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics |
title_full |
Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics |
title_fullStr |
Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics |
title_full_unstemmed |
Isolation and Identification of Soil Bacteria from Extreme Environments of Chile and Their Plant Beneficial Characteristics |
title_sort |
isolation and identification of soil bacteria from extreme environments of chile and their plant beneficial characteristics |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466141/ http://www.ncbi.nlm.nih.gov/pubmed/32785053 https://doi.org/10.3390/microorganisms8081213 |
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ENVELOPE(-59.713,-59.713,-62.374,-62.374) |
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Coppermine Peninsula |
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Coppermine Peninsula |
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Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Microorganisms |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466141/ http://www.ncbi.nlm.nih.gov/pubmed/32785053 http://dx.doi.org/10.3390/microorganisms8081213 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
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https://doi.org/10.3390/microorganisms8081213 |
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Microorganisms |
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1213 |
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1766256323638329344 |