Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon
Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antivi...
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ftpubmed:oai:pubmedcentral.nih.gov:7459813 2023-05-15T15:32:18+02:00 Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon Cárdenas, Constanza Guzmán, Fanny Carmona, Marisela Muñoz, Cristian Nilo, Luis Labra, Alvaro Marshall, Sergio H. 2020-07-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459813/ http://www.ncbi.nlm.nih.gov/pubmed/32717804 https://doi.org/10.3390/pathogens9080600 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459813/ http://www.ncbi.nlm.nih.gov/pubmed/32717804 http://dx.doi.org/10.3390/pathogens9080600 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Pathogens Article Text 2020 ftpubmed https://doi.org/10.3390/pathogens9080600 2020-09-06T00:53:49Z Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture. Text Atlantic salmon Salmo salar PubMed Central (PMC) Pathogens 9 8 600 |
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Article Cárdenas, Constanza Guzmán, Fanny Carmona, Marisela Muñoz, Cristian Nilo, Luis Labra, Alvaro Marshall, Sergio H. Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
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Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture. |
format |
Text |
author |
Cárdenas, Constanza Guzmán, Fanny Carmona, Marisela Muñoz, Cristian Nilo, Luis Labra, Alvaro Marshall, Sergio H. |
author_facet |
Cárdenas, Constanza Guzmán, Fanny Carmona, Marisela Muñoz, Cristian Nilo, Luis Labra, Alvaro Marshall, Sergio H. |
author_sort |
Cárdenas, Constanza |
title |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_short |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_full |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_fullStr |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_full_unstemmed |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_sort |
synthetic peptides as a promising alternative to control viral infections in atlantic salmon |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459813/ http://www.ncbi.nlm.nih.gov/pubmed/32717804 https://doi.org/10.3390/pathogens9080600 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Pathogens |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7459813/ http://www.ncbi.nlm.nih.gov/pubmed/32717804 http://dx.doi.org/10.3390/pathogens9080600 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/pathogens9080600 |
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Pathogens |
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9 |
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8 |
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600 |
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1766362817541177344 |