Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment
Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing chang...
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ftpubmed:oai:pubmedcentral.nih.gov:7451513 2023-05-15T14:54:41+02:00 Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment Buongiorno, Joy Sipes, Katie Wasmund, Kenneth Loy, Alexander Lloyd, Karen G. 2020-08-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451513/ http://www.ncbi.nlm.nih.gov/pubmed/32853201 https://doi.org/10.1371/journal.pone.0234839 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451513/ http://www.ncbi.nlm.nih.gov/pubmed/32853201 http://dx.doi.org/10.1371/journal.pone.0234839 © 2020 Buongiorno et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY PLoS One Research Article Text 2020 ftpubmed https://doi.org/10.1371/journal.pone.0234839 2020-09-06T00:41:48Z Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing change. This study combined 16S rRNA gene analysis, metagenome-assembled genomes (MAGs), and genome-resolved metatranscriptomics uncovered the in situ abundance and transcriptional activity of Woeseiales with burial in four shallow sediment sites of Kongsfjorden and Van Keulenfjorden of Svalbard (79°N). We present five novel Woeseiales MAGs and show transcriptional evidence for metabolic plasticity during burial, including sulfur oxidation with reverse dissimilatory sulfite reductase (dsrAB) down to 4 cm depth and nitrite reduction down to 6 cm depth. A single stress protein, spore protein SP21 (hspA), had a tenfold higher mRNA abundance than any other transcript, and was a hundredfold higher on average than other transcripts. At three out of the four sites, SP21 transcript abundance increased with depth, while total mRNA abundance and richness decreased, indicating a shift in investment from metabolism and other cellular processes to build-up of spore protein SP21. The SP21 gene in MAGs was often flanked by genes involved in membrane-associated stress response. The ability of Woeseiales to shift from sulfur oxidation to nitrite reduction with burial into marine sediments with decreasing access to overlying oxic bottom waters, as well as enter into a dormant state dominated by SP21, may account for its ubiquity and high abundance in marine sediments worldwide, including those of the rapidly shifting Arctic. Text Arctic Kongsfjord* Kongsfjorden Svalbard Van Keulenfjorden PubMed Central (PMC) Arctic Svalbard Van Keulenfjorden ENVELOPE(15.489,15.489,77.559,77.559) PLOS ONE 15 8 e0234839 |
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English |
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Research Article |
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Research Article Buongiorno, Joy Sipes, Katie Wasmund, Kenneth Loy, Alexander Lloyd, Karen G. Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
topic_facet |
Research Article |
description |
Distinct lineages of Gammaproteobacteria clade Woeseiales are globally distributed in marine sediments, based on metagenomic and 16S rRNA gene analysis. Yet little is known about why they are dominant or their ecological role in Arctic fjord sediments, where glacial retreat is rapidly imposing change. This study combined 16S rRNA gene analysis, metagenome-assembled genomes (MAGs), and genome-resolved metatranscriptomics uncovered the in situ abundance and transcriptional activity of Woeseiales with burial in four shallow sediment sites of Kongsfjorden and Van Keulenfjorden of Svalbard (79°N). We present five novel Woeseiales MAGs and show transcriptional evidence for metabolic plasticity during burial, including sulfur oxidation with reverse dissimilatory sulfite reductase (dsrAB) down to 4 cm depth and nitrite reduction down to 6 cm depth. A single stress protein, spore protein SP21 (hspA), had a tenfold higher mRNA abundance than any other transcript, and was a hundredfold higher on average than other transcripts. At three out of the four sites, SP21 transcript abundance increased with depth, while total mRNA abundance and richness decreased, indicating a shift in investment from metabolism and other cellular processes to build-up of spore protein SP21. The SP21 gene in MAGs was often flanked by genes involved in membrane-associated stress response. The ability of Woeseiales to shift from sulfur oxidation to nitrite reduction with burial into marine sediments with decreasing access to overlying oxic bottom waters, as well as enter into a dormant state dominated by SP21, may account for its ubiquity and high abundance in marine sediments worldwide, including those of the rapidly shifting Arctic. |
format |
Text |
author |
Buongiorno, Joy Sipes, Katie Wasmund, Kenneth Loy, Alexander Lloyd, Karen G. |
author_facet |
Buongiorno, Joy Sipes, Katie Wasmund, Kenneth Loy, Alexander Lloyd, Karen G. |
author_sort |
Buongiorno, Joy |
title |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_short |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_full |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_fullStr |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_full_unstemmed |
Woeseiales transcriptional response to shallow burial in Arctic fjord surface sediment |
title_sort |
woeseiales transcriptional response to shallow burial in arctic fjord surface sediment |
publisher |
Public Library of Science |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451513/ http://www.ncbi.nlm.nih.gov/pubmed/32853201 https://doi.org/10.1371/journal.pone.0234839 |
long_lat |
ENVELOPE(15.489,15.489,77.559,77.559) |
geographic |
Arctic Svalbard Van Keulenfjorden |
geographic_facet |
Arctic Svalbard Van Keulenfjorden |
genre |
Arctic Kongsfjord* Kongsfjorden Svalbard Van Keulenfjorden |
genre_facet |
Arctic Kongsfjord* Kongsfjorden Svalbard Van Keulenfjorden |
op_source |
PLoS One |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7451513/ http://www.ncbi.nlm.nih.gov/pubmed/32853201 http://dx.doi.org/10.1371/journal.pone.0234839 |
op_rights |
© 2020 Buongiorno et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0234839 |
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PLOS ONE |
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15 |
container_issue |
8 |
container_start_page |
e0234839 |
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