Metabolome of canine and human saliva: a non-targeted metabolomics study
INTRODUCTION: Saliva metabolites are suggested to reflect the health status of an individual in humans. The same could be true with the dog (Canis lupus familiaris), an important animal model of human disease, but its saliva metabolome is unknown. As a non-invasive sample, canine saliva could offer...
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ftpubmed:oai:pubmedcentral.nih.gov:7447669 2023-05-15T15:51:05+02:00 Metabolome of canine and human saliva: a non-targeted metabolomics study Turunen, Soile Puurunen, Jenni Auriola, Seppo Kullaa, Arja M. Kärkkäinen, Olli Lohi, Hannes Hanhineva, Kati 2020-08-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447669/ http://www.ncbi.nlm.nih.gov/pubmed/32840693 https://doi.org/10.1007/s11306-020-01711-0 en eng Springer US http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447669/ http://www.ncbi.nlm.nih.gov/pubmed/32840693 http://dx.doi.org/10.1007/s11306-020-01711-0 © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Metabolomics Original Article Text 2020 ftpubmed https://doi.org/10.1007/s11306-020-01711-0 2020-09-06T00:38:27Z INTRODUCTION: Saliva metabolites are suggested to reflect the health status of an individual in humans. The same could be true with the dog (Canis lupus familiaris), an important animal model of human disease, but its saliva metabolome is unknown. As a non-invasive sample, canine saliva could offer a new alternative material for research to reveal molecular mechanisms of different (patho)physiological stages, and for veterinary medicine to monitor dogs’ health trajectories. OBJECTIVES: To investigate and characterize the metabolite composition of dog and human saliva in a non-targeted manner. METHODS: Stimulated saliva was collected from 13 privately-owned dogs and from 14 human individuals. We used a non-targeted ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-qTOF-MS) method to measure metabolite profiles from saliva samples. RESULTS: We identified and classified a total of 211 endogenous and exogenous salivary metabolites. The compounds included amino acids, amino acid derivatives, biogenic amines, nucleic acid subunits, lipids, organic acids, small peptides as well as other metabolites, like metabolic waste molecules and other chemicals. Our results reveal a distinct metabolite profile of dog and human saliva as 25 lipid compounds were identified only in canine saliva and eight dipeptides only in human saliva. In addition, we observed large variation in ion abundance within and between the identified saliva metabolites in dog and human. CONCLUSION: The results suggest that non-targeted metabolomics approach utilizing UHPLC-qTOF-MS can detect a wide range of small compounds in dog and human saliva with partially overlapping metabolite composition. The identified metabolites indicate that canine saliva is potentially a versatile material for the discovery of biomarkers for dog welfare. However, this profile is not complete, and dog saliva needs to be investigated in the future with other analytical platforms to characterize the whole canine saliva metabolome. ... Text Canis lupus PubMed Central (PMC) Metabolomics 16 9 |
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Original Article Turunen, Soile Puurunen, Jenni Auriola, Seppo Kullaa, Arja M. Kärkkäinen, Olli Lohi, Hannes Hanhineva, Kati Metabolome of canine and human saliva: a non-targeted metabolomics study |
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Original Article |
description |
INTRODUCTION: Saliva metabolites are suggested to reflect the health status of an individual in humans. The same could be true with the dog (Canis lupus familiaris), an important animal model of human disease, but its saliva metabolome is unknown. As a non-invasive sample, canine saliva could offer a new alternative material for research to reveal molecular mechanisms of different (patho)physiological stages, and for veterinary medicine to monitor dogs’ health trajectories. OBJECTIVES: To investigate and characterize the metabolite composition of dog and human saliva in a non-targeted manner. METHODS: Stimulated saliva was collected from 13 privately-owned dogs and from 14 human individuals. We used a non-targeted ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-qTOF-MS) method to measure metabolite profiles from saliva samples. RESULTS: We identified and classified a total of 211 endogenous and exogenous salivary metabolites. The compounds included amino acids, amino acid derivatives, biogenic amines, nucleic acid subunits, lipids, organic acids, small peptides as well as other metabolites, like metabolic waste molecules and other chemicals. Our results reveal a distinct metabolite profile of dog and human saliva as 25 lipid compounds were identified only in canine saliva and eight dipeptides only in human saliva. In addition, we observed large variation in ion abundance within and between the identified saliva metabolites in dog and human. CONCLUSION: The results suggest that non-targeted metabolomics approach utilizing UHPLC-qTOF-MS can detect a wide range of small compounds in dog and human saliva with partially overlapping metabolite composition. The identified metabolites indicate that canine saliva is potentially a versatile material for the discovery of biomarkers for dog welfare. However, this profile is not complete, and dog saliva needs to be investigated in the future with other analytical platforms to characterize the whole canine saliva metabolome. ... |
format |
Text |
author |
Turunen, Soile Puurunen, Jenni Auriola, Seppo Kullaa, Arja M. Kärkkäinen, Olli Lohi, Hannes Hanhineva, Kati |
author_facet |
Turunen, Soile Puurunen, Jenni Auriola, Seppo Kullaa, Arja M. Kärkkäinen, Olli Lohi, Hannes Hanhineva, Kati |
author_sort |
Turunen, Soile |
title |
Metabolome of canine and human saliva: a non-targeted metabolomics study |
title_short |
Metabolome of canine and human saliva: a non-targeted metabolomics study |
title_full |
Metabolome of canine and human saliva: a non-targeted metabolomics study |
title_fullStr |
Metabolome of canine and human saliva: a non-targeted metabolomics study |
title_full_unstemmed |
Metabolome of canine and human saliva: a non-targeted metabolomics study |
title_sort |
metabolome of canine and human saliva: a non-targeted metabolomics study |
publisher |
Springer US |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447669/ http://www.ncbi.nlm.nih.gov/pubmed/32840693 https://doi.org/10.1007/s11306-020-01711-0 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Metabolomics |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7447669/ http://www.ncbi.nlm.nih.gov/pubmed/32840693 http://dx.doi.org/10.1007/s11306-020-01711-0 |
op_rights |
© The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
op_doi |
https://doi.org/10.1007/s11306-020-01711-0 |
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Metabolomics |
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16 |
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9 |
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1766386142104518656 |