Survival time of Leptospira kirschneri on strawberries
In the past decade, two leptospirosis outbreaks occurred among strawberry harvesters in Germany, with 13, and 45 reported cases respectively. In both outbreaks, common voles (Microtus arvalis) infected with Leptospira kischneri serovar Grippotyphosa were identified as the most likely outbreak source...
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ftpubmed:oai:pubmedcentral.nih.gov:7425971 2023-05-15T17:12:39+02:00 Survival time of Leptospira kirschneri on strawberries Tekemen, Duygu Franz, Mathias Bier, Nadja S. Richter, Martin Nöckler, Karsten Luge, Enno Mayer-Scholl, Anne 2020-08-13 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425971/ http://www.ncbi.nlm.nih.gov/pubmed/32790762 https://doi.org/10.1371/journal.pone.0237466 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425971/ http://www.ncbi.nlm.nih.gov/pubmed/32790762 http://dx.doi.org/10.1371/journal.pone.0237466 © 2020 Tekemen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY PLoS One Research Article Text 2020 ftpubmed https://doi.org/10.1371/journal.pone.0237466 2020-08-23T00:29:30Z In the past decade, two leptospirosis outbreaks occurred among strawberry harvesters in Germany, with 13, and 45 reported cases respectively. In both outbreaks, common voles (Microtus arvalis) infected with Leptospira kischneri serovar Grippotyphosa were identified as the most likely outbreak source. In an univariate analysis, eating unwashed strawberries was identified as one of the risk factors associated with Leptospira infection. The aim of this study was to evaluate the survival time of L. kirschneri serovar Grippotyphosa on strawberries under varying conditions. Strawberries were spiked with 5x10(9) of both a laboratory reference strain (strain Moskva V) and an outbreak field strain (94-6/2007) of L. kirschneri serovar Grippotyphosa sequence type 110. Survival times were investigated in a fully crossed design with three incubation times (2h, 4h, 6h and 8h) and three temperatures (15°C, 21°C and 25°C) with three replicated for each condition. A wash protocol was developed and recovered Leptospira were determined by qPCR, dark field microscopy and culturing. Viable L. kirschneri of both the reference strain and the field strain were identified in all samples at 25°C and an incubation time of 2h, but only 1/9 (11%) and 4/9 (44%) of the samples incubated at 15°C were positive, respectively. Both reference and field strain were viable only in 2/9 (22%) at 25° after 6h. After an 8h incubation, viable Leptospira could not be identified on the surface of the strawberries or within the fruit for any of the tested conditions. Based on these results, the exposure risk of consumers to viable Leptospira spp. through the consumption of strawberries bought at the retail level is most likely very low. However, there is a potential risk of Leptospira infection by consumption of strawberries on pick-your-own farms. Text Microtus arvalis PubMed Central (PMC) PLOS ONE 15 8 e0237466 |
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Research Article Tekemen, Duygu Franz, Mathias Bier, Nadja S. Richter, Martin Nöckler, Karsten Luge, Enno Mayer-Scholl, Anne Survival time of Leptospira kirschneri on strawberries |
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Research Article |
description |
In the past decade, two leptospirosis outbreaks occurred among strawberry harvesters in Germany, with 13, and 45 reported cases respectively. In both outbreaks, common voles (Microtus arvalis) infected with Leptospira kischneri serovar Grippotyphosa were identified as the most likely outbreak source. In an univariate analysis, eating unwashed strawberries was identified as one of the risk factors associated with Leptospira infection. The aim of this study was to evaluate the survival time of L. kirschneri serovar Grippotyphosa on strawberries under varying conditions. Strawberries were spiked with 5x10(9) of both a laboratory reference strain (strain Moskva V) and an outbreak field strain (94-6/2007) of L. kirschneri serovar Grippotyphosa sequence type 110. Survival times were investigated in a fully crossed design with three incubation times (2h, 4h, 6h and 8h) and three temperatures (15°C, 21°C and 25°C) with three replicated for each condition. A wash protocol was developed and recovered Leptospira were determined by qPCR, dark field microscopy and culturing. Viable L. kirschneri of both the reference strain and the field strain were identified in all samples at 25°C and an incubation time of 2h, but only 1/9 (11%) and 4/9 (44%) of the samples incubated at 15°C were positive, respectively. Both reference and field strain were viable only in 2/9 (22%) at 25° after 6h. After an 8h incubation, viable Leptospira could not be identified on the surface of the strawberries or within the fruit for any of the tested conditions. Based on these results, the exposure risk of consumers to viable Leptospira spp. through the consumption of strawberries bought at the retail level is most likely very low. However, there is a potential risk of Leptospira infection by consumption of strawberries on pick-your-own farms. |
format |
Text |
author |
Tekemen, Duygu Franz, Mathias Bier, Nadja S. Richter, Martin Nöckler, Karsten Luge, Enno Mayer-Scholl, Anne |
author_facet |
Tekemen, Duygu Franz, Mathias Bier, Nadja S. Richter, Martin Nöckler, Karsten Luge, Enno Mayer-Scholl, Anne |
author_sort |
Tekemen, Duygu |
title |
Survival time of Leptospira kirschneri on strawberries |
title_short |
Survival time of Leptospira kirschneri on strawberries |
title_full |
Survival time of Leptospira kirschneri on strawberries |
title_fullStr |
Survival time of Leptospira kirschneri on strawberries |
title_full_unstemmed |
Survival time of Leptospira kirschneri on strawberries |
title_sort |
survival time of leptospira kirschneri on strawberries |
publisher |
Public Library of Science |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425971/ http://www.ncbi.nlm.nih.gov/pubmed/32790762 https://doi.org/10.1371/journal.pone.0237466 |
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Microtus arvalis |
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Microtus arvalis |
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PLoS One |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425971/ http://www.ncbi.nlm.nih.gov/pubmed/32790762 http://dx.doi.org/10.1371/journal.pone.0237466 |
op_rights |
© 2020 Tekemen et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
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https://doi.org/10.1371/journal.pone.0237466 |
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