Discovery of Two Novel Negeviruses in a Dungfly Collected from the Arctic
Negeviruses are a proposed group of insect-specific viruses that can be separated into two distinct phylogenetic clades, Nelorpivirus and Sandewavirus. Negeviruses are well-known for their wide geographic distribution and broad host range among hematophagous insects. In this study, the full genomes...
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ftpubmed:oai:pubmedcentral.nih.gov:7412485 2023-05-15T14:59:17+02:00 Discovery of Two Novel Negeviruses in a Dungfly Collected from the Arctic Lu, Gang Ye, Zhuang-Xin He, Yu-Juan Zhang, Yan Wang, Xin Huang, Hai-Jian Zhuo, Ji-Chong Sun, Zong-Tao Yan, Fei Chen, Jian-Ping Zhang, Chuan-Xi Li, Jun-Min 2020-06-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412485/ http://www.ncbi.nlm.nih.gov/pubmed/32604989 https://doi.org/10.3390/v12070692 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412485/ http://www.ncbi.nlm.nih.gov/pubmed/32604989 http://dx.doi.org/10.3390/v12070692 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Viruses Article Text 2020 ftpubmed https://doi.org/10.3390/v12070692 2020-08-30T00:24:49Z Negeviruses are a proposed group of insect-specific viruses that can be separated into two distinct phylogenetic clades, Nelorpivirus and Sandewavirus. Negeviruses are well-known for their wide geographic distribution and broad host range among hematophagous insects. In this study, the full genomes of two novel negeviruses from each of these clades were identified by RNA extraction and sequencing from a single dungfly (Scathophaga furcata) collected from the Arctic Yellow River Station, where these genomes are the first negeviruses from cold zone regions to be discovered. Nelorpivirus dungfly1 (NVD1) and Sandewavirus dungfly1 (SVD1) have the typical negevirus genome organization and there was a very high coverage of viral transcripts. Small interfering RNAs derived from both viruses were readily detected in S. furcata, clearly showing that negeviruses are targeted by the host antiviral RNA interference (RNAi) pathway. These results and subsequent in silico analysis (studies) of public database and published virome data showed that the hosts of nege-like viruses include insects belonging to many orders as well as various non-insects in addition to the hematophagous insects previously reported. Phylogenetic analysis reveals at least three further groups of negeviruses, as well as several poorly resolved solitary branches, filling in the gaps within the two sub-groups of negeviruses and plant-associated viruses in the Kitaviridae. The results of this study will contribute to a better understanding of the geographic distribution, host range, evolution and host antiviral immune responses of negeviruses. Text Arctic PubMed Central (PMC) Arctic Viruses 12 7 692 |
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Article Lu, Gang Ye, Zhuang-Xin He, Yu-Juan Zhang, Yan Wang, Xin Huang, Hai-Jian Zhuo, Ji-Chong Sun, Zong-Tao Yan, Fei Chen, Jian-Ping Zhang, Chuan-Xi Li, Jun-Min Discovery of Two Novel Negeviruses in a Dungfly Collected from the Arctic |
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Negeviruses are a proposed group of insect-specific viruses that can be separated into two distinct phylogenetic clades, Nelorpivirus and Sandewavirus. Negeviruses are well-known for their wide geographic distribution and broad host range among hematophagous insects. In this study, the full genomes of two novel negeviruses from each of these clades were identified by RNA extraction and sequencing from a single dungfly (Scathophaga furcata) collected from the Arctic Yellow River Station, where these genomes are the first negeviruses from cold zone regions to be discovered. Nelorpivirus dungfly1 (NVD1) and Sandewavirus dungfly1 (SVD1) have the typical negevirus genome organization and there was a very high coverage of viral transcripts. Small interfering RNAs derived from both viruses were readily detected in S. furcata, clearly showing that negeviruses are targeted by the host antiviral RNA interference (RNAi) pathway. These results and subsequent in silico analysis (studies) of public database and published virome data showed that the hosts of nege-like viruses include insects belonging to many orders as well as various non-insects in addition to the hematophagous insects previously reported. Phylogenetic analysis reveals at least three further groups of negeviruses, as well as several poorly resolved solitary branches, filling in the gaps within the two sub-groups of negeviruses and plant-associated viruses in the Kitaviridae. The results of this study will contribute to a better understanding of the geographic distribution, host range, evolution and host antiviral immune responses of negeviruses. |
format |
Text |
author |
Lu, Gang Ye, Zhuang-Xin He, Yu-Juan Zhang, Yan Wang, Xin Huang, Hai-Jian Zhuo, Ji-Chong Sun, Zong-Tao Yan, Fei Chen, Jian-Ping Zhang, Chuan-Xi Li, Jun-Min |
author_facet |
Lu, Gang Ye, Zhuang-Xin He, Yu-Juan Zhang, Yan Wang, Xin Huang, Hai-Jian Zhuo, Ji-Chong Sun, Zong-Tao Yan, Fei Chen, Jian-Ping Zhang, Chuan-Xi Li, Jun-Min |
author_sort |
Lu, Gang |
title |
Discovery of Two Novel Negeviruses in a Dungfly Collected from the Arctic |
title_short |
Discovery of Two Novel Negeviruses in a Dungfly Collected from the Arctic |
title_full |
Discovery of Two Novel Negeviruses in a Dungfly Collected from the Arctic |
title_fullStr |
Discovery of Two Novel Negeviruses in a Dungfly Collected from the Arctic |
title_full_unstemmed |
Discovery of Two Novel Negeviruses in a Dungfly Collected from the Arctic |
title_sort |
discovery of two novel negeviruses in a dungfly collected from the arctic |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412485/ http://www.ncbi.nlm.nih.gov/pubmed/32604989 https://doi.org/10.3390/v12070692 |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
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Viruses |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412485/ http://www.ncbi.nlm.nih.gov/pubmed/32604989 http://dx.doi.org/10.3390/v12070692 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/v12070692 |
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