Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion
The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This...
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ftpubmed:oai:pubmedcentral.nih.gov:7401274 2023-05-15T13:54:16+02:00 Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion Pacheco, Luz Verónica Parada, Javier Pérez-Correa, José Ricardo Mariotti-Celis, María Salomé Erpel, Fernanda Zambrano, Angara Palacios, Mauricio 2020-07-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401274/ http://www.ncbi.nlm.nih.gov/pubmed/32650394 https://doi.org/10.3390/md18070353 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401274/ http://www.ncbi.nlm.nih.gov/pubmed/32650394 http://dx.doi.org/10.3390/md18070353 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Mar Drugs Article Text 2020 ftpubmed https://doi.org/10.3390/md18070353 2020-08-16T00:26:11Z The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This research explored the potential of six common Chilean seaweeds to obtain anti-hyperglycemic polyphenol extracts, based on their capacity to inhibit key enzymes related with starch digestion. Ethanol/water hot pressurized liquid extraction (HPLE), which is an environmentally friendly method, was studied and compared to conventional extraction with acetone. Total polyphenols (TP), antioxidant activity, cytotoxicity and inhibition capacity on α-glucosidase and α-amylase were analyzed. Results showed that the Durvillaea antarctica (cochayuyo) acetone extract had the highest TP content (6.7 ± 0.7 mg gallic acid equivalents (GAE)/g dry seaweed), while its HPLE ethanol/water extract showed the highest antioxidant activity (680.1 ± 11.6 μmol E Trolox/g dry seaweed). No extract affected cell viability significantly. Only cochayuyo produced extracts having relevant anti-enzymatic capacity on both studied enzymes, showing a much stronger inhibition to α-glucosidase (even almost 100% at 1000 µg/mL) than to α-amylase. In conclusion, from the Chilean seaweeds considered in this study, cochayuyo is the most suitable for developing functional ingredients to moderate postprandial glycemic response (starchy foods), since it showed a clear enzymatic inhibition capacity and selectivity. Text Antarc* Antarctica PubMed Central (PMC) Marine Drugs 18 7 353 |
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Article Pacheco, Luz Verónica Parada, Javier Pérez-Correa, José Ricardo Mariotti-Celis, María Salomé Erpel, Fernanda Zambrano, Angara Palacios, Mauricio Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
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The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This research explored the potential of six common Chilean seaweeds to obtain anti-hyperglycemic polyphenol extracts, based on their capacity to inhibit key enzymes related with starch digestion. Ethanol/water hot pressurized liquid extraction (HPLE), which is an environmentally friendly method, was studied and compared to conventional extraction with acetone. Total polyphenols (TP), antioxidant activity, cytotoxicity and inhibition capacity on α-glucosidase and α-amylase were analyzed. Results showed that the Durvillaea antarctica (cochayuyo) acetone extract had the highest TP content (6.7 ± 0.7 mg gallic acid equivalents (GAE)/g dry seaweed), while its HPLE ethanol/water extract showed the highest antioxidant activity (680.1 ± 11.6 μmol E Trolox/g dry seaweed). No extract affected cell viability significantly. Only cochayuyo produced extracts having relevant anti-enzymatic capacity on both studied enzymes, showing a much stronger inhibition to α-glucosidase (even almost 100% at 1000 µg/mL) than to α-amylase. In conclusion, from the Chilean seaweeds considered in this study, cochayuyo is the most suitable for developing functional ingredients to moderate postprandial glycemic response (starchy foods), since it showed a clear enzymatic inhibition capacity and selectivity. |
format |
Text |
author |
Pacheco, Luz Verónica Parada, Javier Pérez-Correa, José Ricardo Mariotti-Celis, María Salomé Erpel, Fernanda Zambrano, Angara Palacios, Mauricio |
author_facet |
Pacheco, Luz Verónica Parada, Javier Pérez-Correa, José Ricardo Mariotti-Celis, María Salomé Erpel, Fernanda Zambrano, Angara Palacios, Mauricio |
author_sort |
Pacheco, Luz Verónica |
title |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_short |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_full |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_fullStr |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_full_unstemmed |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_sort |
bioactive polyphenols from southern chile seaweed as inhibitors of enzymes for starch digestion |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401274/ http://www.ncbi.nlm.nih.gov/pubmed/32650394 https://doi.org/10.3390/md18070353 |
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Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401274/ http://www.ncbi.nlm.nih.gov/pubmed/32650394 http://dx.doi.org/10.3390/md18070353 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md18070353 |
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Marine Drugs |
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18 |
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7 |
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353 |
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