Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality
Consumer preference for healthy and sustainable food products has been steadily increasing in recent years. Bivalve mollusks satisfy these characteristics and have captured ever-increasing market shares. However, the expansion of molluscan culture in worldwide and global trade have favored the sprea...
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ftpubmed:oai:pubmedcentral.nih.gov:7350300 2023-05-15T17:54:16+02:00 Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality Mandas, Daniela Salati, Fulvio Polinas, Marta Sanna, Marina Antonella Zobba, Rosanna Burrai, Giovanni Pietro Alberti, Alberto Antuofermo, Elisabetta 2020-06-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350300/ http://www.ncbi.nlm.nih.gov/pubmed/32575736 https://doi.org/10.3390/pathogens9060492 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350300/ http://www.ncbi.nlm.nih.gov/pubmed/32575736 http://dx.doi.org/10.3390/pathogens9060492 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Pathogens Article Text 2020 ftpubmed https://doi.org/10.3390/pathogens9060492 2020-07-19T00:35:52Z Consumer preference for healthy and sustainable food products has been steadily increasing in recent years. Bivalve mollusks satisfy these characteristics and have captured ever-increasing market shares. However, the expansion of molluscan culture in worldwide and global trade have favored the spread of pathogens around the world. Combined with environmental changes and intensive production systems this has contributed to the occurrence of mass mortality episodes, thus posing a threat to the production of different species, including the Pacific oyster Crassotrea gigas. In the San Teodoro lagoon, one of the most devoted lagoons to extensive Pacific oyster aquaculture in Sardinia, a mortality outbreak was observed with an estimated 80% final loss of animal production. A study combining cultural, biomolecular and histopathological methods was conducted: (1) to investigate the presence of different Vibrio species and OsHV-1 in selected oyster tissues (digestive gland, gills, and mantle); (2) to quantify Vibrio aestuarianus and to evaluate the severity of hemocyte infiltration in infected tissues; (3) to produce post-amplification data and evaluating ToxR gene as a target for phylogenetic analyses. Results provide new insights into V. aestuarianus infection related to oyster mortality outbreaks and pave the way to the development of tools for oyster management. Text Pacific oyster PubMed Central (PMC) Pacific Pathogens 9 6 492 |
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Article Mandas, Daniela Salati, Fulvio Polinas, Marta Sanna, Marina Antonella Zobba, Rosanna Burrai, Giovanni Pietro Alberti, Alberto Antuofermo, Elisabetta Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality |
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Consumer preference for healthy and sustainable food products has been steadily increasing in recent years. Bivalve mollusks satisfy these characteristics and have captured ever-increasing market shares. However, the expansion of molluscan culture in worldwide and global trade have favored the spread of pathogens around the world. Combined with environmental changes and intensive production systems this has contributed to the occurrence of mass mortality episodes, thus posing a threat to the production of different species, including the Pacific oyster Crassotrea gigas. In the San Teodoro lagoon, one of the most devoted lagoons to extensive Pacific oyster aquaculture in Sardinia, a mortality outbreak was observed with an estimated 80% final loss of animal production. A study combining cultural, biomolecular and histopathological methods was conducted: (1) to investigate the presence of different Vibrio species and OsHV-1 in selected oyster tissues (digestive gland, gills, and mantle); (2) to quantify Vibrio aestuarianus and to evaluate the severity of hemocyte infiltration in infected tissues; (3) to produce post-amplification data and evaluating ToxR gene as a target for phylogenetic analyses. Results provide new insights into V. aestuarianus infection related to oyster mortality outbreaks and pave the way to the development of tools for oyster management. |
format |
Text |
author |
Mandas, Daniela Salati, Fulvio Polinas, Marta Sanna, Marina Antonella Zobba, Rosanna Burrai, Giovanni Pietro Alberti, Alberto Antuofermo, Elisabetta |
author_facet |
Mandas, Daniela Salati, Fulvio Polinas, Marta Sanna, Marina Antonella Zobba, Rosanna Burrai, Giovanni Pietro Alberti, Alberto Antuofermo, Elisabetta |
author_sort |
Mandas, Daniela |
title |
Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality |
title_short |
Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality |
title_full |
Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality |
title_fullStr |
Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality |
title_full_unstemmed |
Histopathological and Molecular Study of Pacific Oyster Tissues Provides Insights into V. aestuarianus Infection Related to Oyster Mortality |
title_sort |
histopathological and molecular study of pacific oyster tissues provides insights into v. aestuarianus infection related to oyster mortality |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350300/ http://www.ncbi.nlm.nih.gov/pubmed/32575736 https://doi.org/10.3390/pathogens9060492 |
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Pacific |
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Pacific |
genre |
Pacific oyster |
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Pacific oyster |
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Pathogens |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350300/ http://www.ncbi.nlm.nih.gov/pubmed/32575736 http://dx.doi.org/10.3390/pathogens9060492 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/pathogens9060492 |
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Pathogens |
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492 |
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