Limited overall impacts of ectomycorrhizal inoculation on recruitment of boreal trees into Arctic tundra following wildfire belie species-specific responses
We tested whether post-fire seedling establishment of common boreal tree and expanding shrub species at treeline and in Arctic tundra is facilitated by co-migration of boreal forest mycorrhizal fungi. Wildfires are anticipated to facilitate biome shifts at the forest-tundra ecotone by improving seed...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347221/ http://www.ncbi.nlm.nih.gov/pubmed/32645087 https://doi.org/10.1371/journal.pone.0235932 |
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ftpubmed:oai:pubmedcentral.nih.gov:7347221 2023-05-15T15:02:05+02:00 Limited overall impacts of ectomycorrhizal inoculation on recruitment of boreal trees into Arctic tundra following wildfire belie species-specific responses Hewitt, Rebecca E. Chapin, F. Stuart Hollingsworth, Teresa N. Mack, Michelle C. Rocha, Adrian V. Taylor, D. Lee 2020-07-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347221/ http://www.ncbi.nlm.nih.gov/pubmed/32645087 https://doi.org/10.1371/journal.pone.0235932 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347221/ http://www.ncbi.nlm.nih.gov/pubmed/32645087 http://dx.doi.org/10.1371/journal.pone.0235932 https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. CC0 PDM PLoS One Research Article Text 2020 ftpubmed https://doi.org/10.1371/journal.pone.0235932 2020-07-26T00:19:59Z We tested whether post-fire seedling establishment of common boreal tree and expanding shrub species at treeline and in Arctic tundra is facilitated by co-migration of boreal forest mycorrhizal fungi. Wildfires are anticipated to facilitate biome shifts at the forest-tundra ecotone by improving seedbed conditions for recruiting boreal species; at the same time fire alters the composition and availability of mycorrhizal fungi critical to seedling performance. To determine the role of root-associated fungi (RAF) in post-fire seedling recruitment and future biome shifts, we outplanted four dominant boreal tree and shrub species inoculated with one of three treatments at treeline and in tundra: burned boreal forest, unburned boreal forest, or a control treatment of sterilized inoculum. We compared survivorship, growth, and physiological performance of the seedlings in relation to mycorrhizal inoculum treatment and among host species, characterized the RAF communities based on ITS-rDNA sequencing of individual root tips sampled from surviving seedlings, and tested for correlations between RAF composition and the inoculation treatments, host species, and duration of the experiment. We explored correlations between RAF composition and seedling metrics. Both live and sterile autoclaved inoculation treatments had similar effects on seedling survivorship and growth for all species. RAF composition did not vary by treatment, suggesting that most colonization was due to local fungi. However, seedling traits and growth were correlated with RAF species composition, colonization, and the relative abundance of specific RAF taxa. Picea sp. performance in particular showed strong co-variation with RAF metrics. Our results suggest that mycorrhizal co-migration is not a primary limiting factor to boreal seedling recruitment because the experimental provision of inoculum did not affect seedling recruitment; yet, RAF did influence seedling performance, particularly resident RAF at treeline and in tundra, suggesting that mycorrhizal ... Text Arctic Tundra PubMed Central (PMC) Arctic PLOS ONE 15 7 e0235932 |
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English |
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Research Article Hewitt, Rebecca E. Chapin, F. Stuart Hollingsworth, Teresa N. Mack, Michelle C. Rocha, Adrian V. Taylor, D. Lee Limited overall impacts of ectomycorrhizal inoculation on recruitment of boreal trees into Arctic tundra following wildfire belie species-specific responses |
topic_facet |
Research Article |
description |
We tested whether post-fire seedling establishment of common boreal tree and expanding shrub species at treeline and in Arctic tundra is facilitated by co-migration of boreal forest mycorrhizal fungi. Wildfires are anticipated to facilitate biome shifts at the forest-tundra ecotone by improving seedbed conditions for recruiting boreal species; at the same time fire alters the composition and availability of mycorrhizal fungi critical to seedling performance. To determine the role of root-associated fungi (RAF) in post-fire seedling recruitment and future biome shifts, we outplanted four dominant boreal tree and shrub species inoculated with one of three treatments at treeline and in tundra: burned boreal forest, unburned boreal forest, or a control treatment of sterilized inoculum. We compared survivorship, growth, and physiological performance of the seedlings in relation to mycorrhizal inoculum treatment and among host species, characterized the RAF communities based on ITS-rDNA sequencing of individual root tips sampled from surviving seedlings, and tested for correlations between RAF composition and the inoculation treatments, host species, and duration of the experiment. We explored correlations between RAF composition and seedling metrics. Both live and sterile autoclaved inoculation treatments had similar effects on seedling survivorship and growth for all species. RAF composition did not vary by treatment, suggesting that most colonization was due to local fungi. However, seedling traits and growth were correlated with RAF species composition, colonization, and the relative abundance of specific RAF taxa. Picea sp. performance in particular showed strong co-variation with RAF metrics. Our results suggest that mycorrhizal co-migration is not a primary limiting factor to boreal seedling recruitment because the experimental provision of inoculum did not affect seedling recruitment; yet, RAF did influence seedling performance, particularly resident RAF at treeline and in tundra, suggesting that mycorrhizal ... |
format |
Text |
author |
Hewitt, Rebecca E. Chapin, F. Stuart Hollingsworth, Teresa N. Mack, Michelle C. Rocha, Adrian V. Taylor, D. Lee |
author_facet |
Hewitt, Rebecca E. Chapin, F. Stuart Hollingsworth, Teresa N. Mack, Michelle C. Rocha, Adrian V. Taylor, D. Lee |
author_sort |
Hewitt, Rebecca E. |
title |
Limited overall impacts of ectomycorrhizal inoculation on recruitment of boreal trees into Arctic tundra following wildfire belie species-specific responses |
title_short |
Limited overall impacts of ectomycorrhizal inoculation on recruitment of boreal trees into Arctic tundra following wildfire belie species-specific responses |
title_full |
Limited overall impacts of ectomycorrhizal inoculation on recruitment of boreal trees into Arctic tundra following wildfire belie species-specific responses |
title_fullStr |
Limited overall impacts of ectomycorrhizal inoculation on recruitment of boreal trees into Arctic tundra following wildfire belie species-specific responses |
title_full_unstemmed |
Limited overall impacts of ectomycorrhizal inoculation on recruitment of boreal trees into Arctic tundra following wildfire belie species-specific responses |
title_sort |
limited overall impacts of ectomycorrhizal inoculation on recruitment of boreal trees into arctic tundra following wildfire belie species-specific responses |
publisher |
Public Library of Science |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347221/ http://www.ncbi.nlm.nih.gov/pubmed/32645087 https://doi.org/10.1371/journal.pone.0235932 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Tundra |
genre_facet |
Arctic Tundra |
op_source |
PLoS One |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7347221/ http://www.ncbi.nlm.nih.gov/pubmed/32645087 http://dx.doi.org/10.1371/journal.pone.0235932 |
op_rights |
https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
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CC0 PDM |
op_doi |
https://doi.org/10.1371/journal.pone.0235932 |
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PLOS ONE |
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15 |
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7 |
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