Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica
Polar marine ecosystems hold the potential for bioactive compound biodiscovery, based on their untapped macro- and microorganism diversity. Characterization of polar benthic marine invertebrate-associated microbiomes is limited to few studies. This study was motivated by our interest in better under...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345734/ http://www.ncbi.nlm.nih.gov/pubmed/32498449 https://doi.org/10.3390/md18060298 |
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ftpubmed:oai:pubmedcentral.nih.gov:7345734 2023-05-15T13:48:06+02:00 Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica Murray, Alison E. Avalon, Nicole E. Bishop, Lucas Davenport, Karen W. Delage, Erwan Dichosa, Armand E.K. Eveillard, Damien Higham, Mary L. Kokkaliari, Sofia Lo, Chien-Chi Riesenfeld, Christian S. Young, Ryan M. Chain, Patrick S.G. Baker, Bill J. 2020-06-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345734/ http://www.ncbi.nlm.nih.gov/pubmed/32498449 https://doi.org/10.3390/md18060298 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345734/ http://www.ncbi.nlm.nih.gov/pubmed/32498449 http://dx.doi.org/10.3390/md18060298 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Mar Drugs Article Text 2020 ftpubmed https://doi.org/10.3390/md18060298 2020-07-12T00:47:48Z Polar marine ecosystems hold the potential for bioactive compound biodiscovery, based on their untapped macro- and microorganism diversity. Characterization of polar benthic marine invertebrate-associated microbiomes is limited to few studies. This study was motivated by our interest in better understanding the microbiome structure and composition of the ascidian, Synoicum adareanum, in which palmerolide A (PalA), a bioactive macrolide with specificity against melanoma, was isolated. PalA bears structural resemblance to a hybrid nonribosomal peptide-polyketide that has similarities to microbially-produced macrolides. We conducted a spatial survey to assess both PalA levels and microbiome composition in S. adareanum in a region of the Antarctic Peninsula near Anvers Island (64°46′ S, 64°03′ W). PalA was ubiquitous and abundant across a collection of 21 ascidians (3 subsamples each) sampled from seven sites across the Anvers Island Archipelago. The microbiome composition (V3–V4 16S rRNA gene sequence variants) of these 63 samples revealed a core suite of 21 bacterial amplicon sequence variants (ASVs)—20 of which were distinct from regional bacterioplankton. ASV co-occurrence analysis across all 63 samples yielded subgroups of taxa that may be interacting biologically (interacting subsystems) and, although the levels of PalA detected were not found to correlate with specific sequence variants, the core members appeared to occur in a preferred optimum and tolerance range of PalA levels. These results, together with an analysis of the biosynthetic potential of related microbiome taxa, describe a conserved, high-latitude core microbiome with unique composition and substantial promise for natural product biosynthesis that likely influences the ecology of the holobiont. Text Antarc* Antarctic Antarctic Peninsula Antarctica Anvers Island PubMed Central (PMC) Antarctic Antarctic Peninsula Anvers ENVELOPE(-63.500,-63.500,-64.600,-64.600) Anvers Island ENVELOPE(-63.500,-63.500,-64.600,-64.600) Pala ENVELOPE(40.637,40.637,64.896,64.896) The Antarctic Marine Drugs 18 6 298 |
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Article Murray, Alison E. Avalon, Nicole E. Bishop, Lucas Davenport, Karen W. Delage, Erwan Dichosa, Armand E.K. Eveillard, Damien Higham, Mary L. Kokkaliari, Sofia Lo, Chien-Chi Riesenfeld, Christian S. Young, Ryan M. Chain, Patrick S.G. Baker, Bill J. Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
topic_facet |
Article |
description |
Polar marine ecosystems hold the potential for bioactive compound biodiscovery, based on their untapped macro- and microorganism diversity. Characterization of polar benthic marine invertebrate-associated microbiomes is limited to few studies. This study was motivated by our interest in better understanding the microbiome structure and composition of the ascidian, Synoicum adareanum, in which palmerolide A (PalA), a bioactive macrolide with specificity against melanoma, was isolated. PalA bears structural resemblance to a hybrid nonribosomal peptide-polyketide that has similarities to microbially-produced macrolides. We conducted a spatial survey to assess both PalA levels and microbiome composition in S. adareanum in a region of the Antarctic Peninsula near Anvers Island (64°46′ S, 64°03′ W). PalA was ubiquitous and abundant across a collection of 21 ascidians (3 subsamples each) sampled from seven sites across the Anvers Island Archipelago. The microbiome composition (V3–V4 16S rRNA gene sequence variants) of these 63 samples revealed a core suite of 21 bacterial amplicon sequence variants (ASVs)—20 of which were distinct from regional bacterioplankton. ASV co-occurrence analysis across all 63 samples yielded subgroups of taxa that may be interacting biologically (interacting subsystems) and, although the levels of PalA detected were not found to correlate with specific sequence variants, the core members appeared to occur in a preferred optimum and tolerance range of PalA levels. These results, together with an analysis of the biosynthetic potential of related microbiome taxa, describe a conserved, high-latitude core microbiome with unique composition and substantial promise for natural product biosynthesis that likely influences the ecology of the holobiont. |
format |
Text |
author |
Murray, Alison E. Avalon, Nicole E. Bishop, Lucas Davenport, Karen W. Delage, Erwan Dichosa, Armand E.K. Eveillard, Damien Higham, Mary L. Kokkaliari, Sofia Lo, Chien-Chi Riesenfeld, Christian S. Young, Ryan M. Chain, Patrick S.G. Baker, Bill J. |
author_facet |
Murray, Alison E. Avalon, Nicole E. Bishop, Lucas Davenport, Karen W. Delage, Erwan Dichosa, Armand E.K. Eveillard, Damien Higham, Mary L. Kokkaliari, Sofia Lo, Chien-Chi Riesenfeld, Christian S. Young, Ryan M. Chain, Patrick S.G. Baker, Bill J. |
author_sort |
Murray, Alison E. |
title |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_short |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_full |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_fullStr |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_full_unstemmed |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_sort |
uncovering the core microbiome and distribution of palmerolide in synoicum adareanum across the anvers island archipelago, antarctica |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345734/ http://www.ncbi.nlm.nih.gov/pubmed/32498449 https://doi.org/10.3390/md18060298 |
long_lat |
ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(40.637,40.637,64.896,64.896) |
geographic |
Antarctic Antarctic Peninsula Anvers Anvers Island Pala The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula Anvers Anvers Island Pala The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Anvers Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Anvers Island |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7345734/ http://www.ncbi.nlm.nih.gov/pubmed/32498449 http://dx.doi.org/10.3390/md18060298 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/md18060298 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
6 |
container_start_page |
298 |
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1766248597961048064 |