Interaction between ω6 and ω3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles
Atlantic salmon smolts (approx. 20-months old) were fed experimental diets with different combinations of omega-6:omega-3 fatty acids (FAs) (high-ω6, high-ω3, or balanced) and eicosapentaenoic acid plus docosahexaenoic acid (EPA + DHA) levels (0.3, 1.0 or 1.4%) for 12 weeks. Muscle FA (% total FA) r...
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ftpubmed:oai:pubmedcentral.nih.gov:7333962 2023-05-15T15:31:59+02:00 Interaction between ω6 and ω3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles Emam, Mohamed Katan, Tomer Caballero-Solares, Albert Taylor, Richard G. Parrish, Kathleen S. Rise, Matthew L. Parrish, Christopher C. 2020-08-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333962/ http://www.ncbi.nlm.nih.gov/pubmed/32536300 https://doi.org/10.1098/rstb.2019.0648 en eng The Royal Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333962/ http://www.ncbi.nlm.nih.gov/pubmed/32536300 http://dx.doi.org/10.1098/rstb.2019.0648 © 2020 The Author(s) https://royalsociety.org/-/media/journals/author/Licence-to-Publish-20062019-final.pdfhttps://royalsociety.org/journals/ethics-policies/data-sharing-mining/Published by the Royal Society. All rights reserved. Philos Trans R Soc Lond B Biol Sci Articles Text 2020 ftpubmed https://doi.org/10.1098/rstb.2019.0648 2021-08-08T00:21:56Z Atlantic salmon smolts (approx. 20-months old) were fed experimental diets with different combinations of omega-6:omega-3 fatty acids (FAs) (high-ω6, high-ω3, or balanced) and eicosapentaenoic acid plus docosahexaenoic acid (EPA + DHA) levels (0.3, 1.0 or 1.4%) for 12 weeks. Muscle FA (% total FA) reflected dietary C(18)-polyunsaturated FA; however, muscle EPA per cent and content (mg g(−1)) were not different in salmon fed high-ω3 or balanced diets. Muscle DHA per cent was similar among treatments, while DHA content increased in fish fed 1.4% EPA + DHA, compared with those fed 0.3–1.0% EPA + DHA combined with high-ω6 FA. Muscle 20:3ω6 (DGLA) content was highest in those fed high-ω6 with 0.3% EPA + DHA. Quantitative polymerase chain reaction analyses on liver RNA showed that the monounsaturated FA synthesis-related gene, scdb, was upregulated in fish fed 1.0% EPA + DHA with high-ω6 compared to those fed 0.3% EPA + DHA. In high-ω3-fed salmon, liver elovl2 transcript levels were higher with 0.3% EPA + DHA than with 1.0% EPA + DHA. In high-ω6-fed fish, elovl2 did not vary with EPA + DHA levels, but it was positively correlated with muscle ARA, 22:4ω3 and DGLA. These results suggest dietary 18:3ω3 elongation contributed to maintaining muscle EPA + DHA levels despite a two- to threefold change in dietary proportions, while 18:2ω6 with 0.3% EPA + DHA increased muscle DGLA more than arachidonic acid (ARA). Positive correlations between hepatic elovl2 and fabp10a with muscle ω6:ω3 and EPA + DHA + ARA, respectively, were confirmed by reanalysing data from a previous salmon trial with lower variations in dietary EPA + DHA and ω6:ω3 ratios. This article is part of the theme issue ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids’. Text Atlantic salmon PubMed Central (PMC) Philosophical Transactions of the Royal Society B: Biological Sciences 375 1804 20190648 |
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Articles Emam, Mohamed Katan, Tomer Caballero-Solares, Albert Taylor, Richard G. Parrish, Kathleen S. Rise, Matthew L. Parrish, Christopher C. Interaction between ω6 and ω3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
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Articles |
description |
Atlantic salmon smolts (approx. 20-months old) were fed experimental diets with different combinations of omega-6:omega-3 fatty acids (FAs) (high-ω6, high-ω3, or balanced) and eicosapentaenoic acid plus docosahexaenoic acid (EPA + DHA) levels (0.3, 1.0 or 1.4%) for 12 weeks. Muscle FA (% total FA) reflected dietary C(18)-polyunsaturated FA; however, muscle EPA per cent and content (mg g(−1)) were not different in salmon fed high-ω3 or balanced diets. Muscle DHA per cent was similar among treatments, while DHA content increased in fish fed 1.4% EPA + DHA, compared with those fed 0.3–1.0% EPA + DHA combined with high-ω6 FA. Muscle 20:3ω6 (DGLA) content was highest in those fed high-ω6 with 0.3% EPA + DHA. Quantitative polymerase chain reaction analyses on liver RNA showed that the monounsaturated FA synthesis-related gene, scdb, was upregulated in fish fed 1.0% EPA + DHA with high-ω6 compared to those fed 0.3% EPA + DHA. In high-ω3-fed salmon, liver elovl2 transcript levels were higher with 0.3% EPA + DHA than with 1.0% EPA + DHA. In high-ω6-fed fish, elovl2 did not vary with EPA + DHA levels, but it was positively correlated with muscle ARA, 22:4ω3 and DGLA. These results suggest dietary 18:3ω3 elongation contributed to maintaining muscle EPA + DHA levels despite a two- to threefold change in dietary proportions, while 18:2ω6 with 0.3% EPA + DHA increased muscle DGLA more than arachidonic acid (ARA). Positive correlations between hepatic elovl2 and fabp10a with muscle ω6:ω3 and EPA + DHA + ARA, respectively, were confirmed by reanalysing data from a previous salmon trial with lower variations in dietary EPA + DHA and ω6:ω3 ratios. This article is part of the theme issue ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids’. |
format |
Text |
author |
Emam, Mohamed Katan, Tomer Caballero-Solares, Albert Taylor, Richard G. Parrish, Kathleen S. Rise, Matthew L. Parrish, Christopher C. |
author_facet |
Emam, Mohamed Katan, Tomer Caballero-Solares, Albert Taylor, Richard G. Parrish, Kathleen S. Rise, Matthew L. Parrish, Christopher C. |
author_sort |
Emam, Mohamed |
title |
Interaction between ω6 and ω3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_short |
Interaction between ω6 and ω3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_full |
Interaction between ω6 and ω3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_fullStr |
Interaction between ω6 and ω3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_full_unstemmed |
Interaction between ω6 and ω3 fatty acids of different chain lengths regulates Atlantic salmon hepatic gene expression and muscle fatty acid profiles |
title_sort |
interaction between ω6 and ω3 fatty acids of different chain lengths regulates atlantic salmon hepatic gene expression and muscle fatty acid profiles |
publisher |
The Royal Society |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333962/ http://www.ncbi.nlm.nih.gov/pubmed/32536300 https://doi.org/10.1098/rstb.2019.0648 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Philos Trans R Soc Lond B Biol Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7333962/ http://www.ncbi.nlm.nih.gov/pubmed/32536300 http://dx.doi.org/10.1098/rstb.2019.0648 |
op_rights |
© 2020 The Author(s) https://royalsociety.org/-/media/journals/author/Licence-to-Publish-20062019-final.pdfhttps://royalsociety.org/journals/ethics-policies/data-sharing-mining/Published by the Royal Society. All rights reserved. |
op_doi |
https://doi.org/10.1098/rstb.2019.0648 |
container_title |
Philosophical Transactions of the Royal Society B: Biological Sciences |
container_volume |
375 |
container_issue |
1804 |
container_start_page |
20190648 |
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1766362473104932864 |