Molecular Network-Guided Alkaloid Profiling of Aerial Parts of Papaver nudicaule L. Using LC-HRMS
Papaver nudicaule L. (Iceland poppy) is widely used for ornamental purposes. A previous study demonstrated the alleviation of lipopolysaccharide-induced inflammation mediated by P. nudicaule extract through nuclear factor-kappa B and signal transducer and activator of transcription 3 inactivation. A...
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ftpubmed:oai:pubmedcentral.nih.gov:7321159 2023-05-15T16:50:38+02:00 Molecular Network-Guided Alkaloid Profiling of Aerial Parts of Papaver nudicaule L. Using LC-HRMS Song, Kwangho Oh, Jae-Hyeon Lee, Min Young Lee, Seok-Geun Ha, In Jin 2020-06-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321159/ http://www.ncbi.nlm.nih.gov/pubmed/32517053 https://doi.org/10.3390/molecules25112636 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321159/ http://www.ncbi.nlm.nih.gov/pubmed/32517053 http://dx.doi.org/10.3390/molecules25112636 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Molecules Article Text 2020 ftpubmed https://doi.org/10.3390/molecules25112636 2020-07-12T00:21:19Z Papaver nudicaule L. (Iceland poppy) is widely used for ornamental purposes. A previous study demonstrated the alleviation of lipopolysaccharide-induced inflammation mediated by P. nudicaule extract through nuclear factor-kappa B and signal transducer and activator of transcription 3 inactivation. As isoquinoline alkaloids are chemical markers and bioactive constituents of Papaver species, the present study investigated the alkaloid profile of aerial parts of five P. nudicaule cultivars with different flower colors and a P. rhoeas cropped for two years. A combination of liquid chromatography high-resolution mass spectrometry and molecular networking was used to cluster isoquinoline alkaloids in the species and highlight the possible metabolites. Aside from the 12 compounds, including rotundine, muramine, and allocryptopine, identified from Global Natural Products Social library and reported information, 46 structurally related metabolites were quantitatively investigated. Forty-two and 16 compounds were proposed for chemical profiles of P. nudicaule and P. rhoeas, respectively. Some species-specific metabolites showed similar fragmentation patterns. The alkaloid abundance of P. nudicaule differed depending on the flower color, and the possible chemical markers were proposed. These results show that molecular networking-guided dereplication allows investigation of unidentified metabolites. The derived chemical profile may facilitate evaluation of P. nudicaule quality for pharmacological applications. Text Iceland PubMed Central (PMC) Molecules 25 11 2636 |
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Article Song, Kwangho Oh, Jae-Hyeon Lee, Min Young Lee, Seok-Geun Ha, In Jin Molecular Network-Guided Alkaloid Profiling of Aerial Parts of Papaver nudicaule L. Using LC-HRMS |
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Papaver nudicaule L. (Iceland poppy) is widely used for ornamental purposes. A previous study demonstrated the alleviation of lipopolysaccharide-induced inflammation mediated by P. nudicaule extract through nuclear factor-kappa B and signal transducer and activator of transcription 3 inactivation. As isoquinoline alkaloids are chemical markers and bioactive constituents of Papaver species, the present study investigated the alkaloid profile of aerial parts of five P. nudicaule cultivars with different flower colors and a P. rhoeas cropped for two years. A combination of liquid chromatography high-resolution mass spectrometry and molecular networking was used to cluster isoquinoline alkaloids in the species and highlight the possible metabolites. Aside from the 12 compounds, including rotundine, muramine, and allocryptopine, identified from Global Natural Products Social library and reported information, 46 structurally related metabolites were quantitatively investigated. Forty-two and 16 compounds were proposed for chemical profiles of P. nudicaule and P. rhoeas, respectively. Some species-specific metabolites showed similar fragmentation patterns. The alkaloid abundance of P. nudicaule differed depending on the flower color, and the possible chemical markers were proposed. These results show that molecular networking-guided dereplication allows investigation of unidentified metabolites. The derived chemical profile may facilitate evaluation of P. nudicaule quality for pharmacological applications. |
format |
Text |
author |
Song, Kwangho Oh, Jae-Hyeon Lee, Min Young Lee, Seok-Geun Ha, In Jin |
author_facet |
Song, Kwangho Oh, Jae-Hyeon Lee, Min Young Lee, Seok-Geun Ha, In Jin |
author_sort |
Song, Kwangho |
title |
Molecular Network-Guided Alkaloid Profiling of Aerial Parts of Papaver nudicaule L. Using LC-HRMS |
title_short |
Molecular Network-Guided Alkaloid Profiling of Aerial Parts of Papaver nudicaule L. Using LC-HRMS |
title_full |
Molecular Network-Guided Alkaloid Profiling of Aerial Parts of Papaver nudicaule L. Using LC-HRMS |
title_fullStr |
Molecular Network-Guided Alkaloid Profiling of Aerial Parts of Papaver nudicaule L. Using LC-HRMS |
title_full_unstemmed |
Molecular Network-Guided Alkaloid Profiling of Aerial Parts of Papaver nudicaule L. Using LC-HRMS |
title_sort |
molecular network-guided alkaloid profiling of aerial parts of papaver nudicaule l. using lc-hrms |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321159/ http://www.ncbi.nlm.nih.gov/pubmed/32517053 https://doi.org/10.3390/molecules25112636 |
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Iceland |
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Iceland |
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Molecules |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7321159/ http://www.ncbi.nlm.nih.gov/pubmed/32517053 http://dx.doi.org/10.3390/molecules25112636 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
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https://doi.org/10.3390/molecules25112636 |
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Molecules |
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2636 |
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