DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment
Adipocytes play a central role in overall energy homeostasis and are important contributors to the immune system. Fatty acids (FAs) act as signaling molecules capable to modulate adipocyte metabolism and functions. To identify the effects of two commonly used FAs in Atlantic salmon diets, primary ad...
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ftpubmed:oai:pubmedcentral.nih.gov:7312884 2023-05-15T15:30:29+02:00 DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment Bou, Marta Torgersen, Jacob Seilø Østbye, Tone-Kari Knutsdatter Ruyter, Bente Wang, Xinxia Škugor, Stanko Kristiansen, Inger Øien Todorčević, Marijana 2020-06-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312884/ http://www.ncbi.nlm.nih.gov/pubmed/32521827 https://doi.org/10.3390/ijms21114101 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312884/ http://www.ncbi.nlm.nih.gov/pubmed/32521827 http://dx.doi.org/10.3390/ijms21114101 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Int J Mol Sci Article Text 2020 ftpubmed https://doi.org/10.3390/ijms21114101 2020-07-05T00:42:02Z Adipocytes play a central role in overall energy homeostasis and are important contributors to the immune system. Fatty acids (FAs) act as signaling molecules capable to modulate adipocyte metabolism and functions. To identify the effects of two commonly used FAs in Atlantic salmon diets, primary adipocytes were cultured in the presence of oleic (OA) or docosahexaenoic (DHA) acid. DHA decreased adipocyte lipid droplet number and area compared to OA. The increase in lipid load in OA treated adipocytes was paralleled by an increase in iNOS activity and mitochondrial SOD2-GFP activity, which was probably directed to counteract increase in oxidative stress. Under lipopolysaccharide (LPS)-induced inflammation, DHA had a greater anti-inflammatory effect than OA, as evidenced by the higher SOD2 activity and the transcriptional regulation of antioxidant enzymes and pro- and anti-inflammatory markers. In addition, DHA maintained a healthy mitochondrial structure under induced inflammation while OA led to elongated mitochondria with a thin thread like structures in adipocytes exposed to LPS. Overall, DHA possess anti-inflammatory properties and protects Atlantic salmon against oxidative stress and limits lipid deposition. Furthermore, DHA plays a key role in protecting mitochondria shape and function. Text Atlantic salmon PubMed Central (PMC) International Journal of Molecular Sciences 21 11 4101 |
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Article Bou, Marta Torgersen, Jacob Seilø Østbye, Tone-Kari Knutsdatter Ruyter, Bente Wang, Xinxia Škugor, Stanko Kristiansen, Inger Øien Todorčević, Marijana DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment |
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description |
Adipocytes play a central role in overall energy homeostasis and are important contributors to the immune system. Fatty acids (FAs) act as signaling molecules capable to modulate adipocyte metabolism and functions. To identify the effects of two commonly used FAs in Atlantic salmon diets, primary adipocytes were cultured in the presence of oleic (OA) or docosahexaenoic (DHA) acid. DHA decreased adipocyte lipid droplet number and area compared to OA. The increase in lipid load in OA treated adipocytes was paralleled by an increase in iNOS activity and mitochondrial SOD2-GFP activity, which was probably directed to counteract increase in oxidative stress. Under lipopolysaccharide (LPS)-induced inflammation, DHA had a greater anti-inflammatory effect than OA, as evidenced by the higher SOD2 activity and the transcriptional regulation of antioxidant enzymes and pro- and anti-inflammatory markers. In addition, DHA maintained a healthy mitochondrial structure under induced inflammation while OA led to elongated mitochondria with a thin thread like structures in adipocytes exposed to LPS. Overall, DHA possess anti-inflammatory properties and protects Atlantic salmon against oxidative stress and limits lipid deposition. Furthermore, DHA plays a key role in protecting mitochondria shape and function. |
format |
Text |
author |
Bou, Marta Torgersen, Jacob Seilø Østbye, Tone-Kari Knutsdatter Ruyter, Bente Wang, Xinxia Škugor, Stanko Kristiansen, Inger Øien Todorčević, Marijana |
author_facet |
Bou, Marta Torgersen, Jacob Seilø Østbye, Tone-Kari Knutsdatter Ruyter, Bente Wang, Xinxia Škugor, Stanko Kristiansen, Inger Øien Todorčević, Marijana |
author_sort |
Bou, Marta |
title |
DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment |
title_short |
DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment |
title_full |
DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment |
title_fullStr |
DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment |
title_full_unstemmed |
DHA Modulates Immune Response and Mitochondrial Function of Atlantic Salmon Adipocytes after LPS Treatment |
title_sort |
dha modulates immune response and mitochondrial function of atlantic salmon adipocytes after lps treatment |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312884/ http://www.ncbi.nlm.nih.gov/pubmed/32521827 https://doi.org/10.3390/ijms21114101 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Int J Mol Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7312884/ http://www.ncbi.nlm.nih.gov/pubmed/32521827 http://dx.doi.org/10.3390/ijms21114101 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/ijms21114101 |
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International Journal of Molecular Sciences |
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21 |
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11 |
container_start_page |
4101 |
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1766360925616472064 |