Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to variation in...
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ftpubmed:oai:pubmedcentral.nih.gov:7295772 2023-05-15T15:27:35+02:00 Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners Anderson, Kelli C. Alix, Maud Charitonidou, Katerina Thorsen, Anders Thorsheim, Grethe Ganias, Kostas Schmidt, Thassya C. dos Santos Kjesbu, Olav Sigurd 2020-06-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295772/ http://www.ncbi.nlm.nih.gov/pubmed/32541862 https://doi.org/10.1038/s41598-020-66601-w en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295772/ http://www.ncbi.nlm.nih.gov/pubmed/32541862 http://dx.doi.org/10.1038/s41598-020-66601-w © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Sci Rep Article Text 2020 ftpubmed https://doi.org/10.1038/s41598-020-66601-w 2020-06-21T00:49:20Z The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to variation in oocyte dynamics between specimens. Atlantic cod (Barents Sea stock) were used to develop the new ‘ultrametric’ method, that is based on the progressive depletion of the vitellogenic oocyte pool relative to the rather constant previtellogenic oocyte (PVO) pool. Fish were subsequently partitioned into one of four categories that accurately reflected changes in their oocyte size frequency distribution characteristics and gonadosomatic index throughout spawning. The ultrametric method overcomes difficulties associated with presence of bimodal oocyte distributions, oocyte tails, lack of clear hiatus region, and presence of free ova, and can be implemented at a single sampling point. Much of the workflow is fully automated, and the technique may circumvent the need for histological analysis depending on the desired outcome. The ultrametric method differs from the traditional autodiametric method in that PVOs can be separated by ultrasonication and then enumerated, and ovarian homogeneity is not a mandatory requirement per se. The method is designed for determinate spawners but might be extended to include indeterminate spawners. Text atlantic cod Barents Sea PubMed Central (PMC) Barents Sea Scientific Reports 10 1 |
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Article Anderson, Kelli C. Alix, Maud Charitonidou, Katerina Thorsen, Anders Thorsheim, Grethe Ganias, Kostas Schmidt, Thassya C. dos Santos Kjesbu, Olav Sigurd Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
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The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to variation in oocyte dynamics between specimens. Atlantic cod (Barents Sea stock) were used to develop the new ‘ultrametric’ method, that is based on the progressive depletion of the vitellogenic oocyte pool relative to the rather constant previtellogenic oocyte (PVO) pool. Fish were subsequently partitioned into one of four categories that accurately reflected changes in their oocyte size frequency distribution characteristics and gonadosomatic index throughout spawning. The ultrametric method overcomes difficulties associated with presence of bimodal oocyte distributions, oocyte tails, lack of clear hiatus region, and presence of free ova, and can be implemented at a single sampling point. Much of the workflow is fully automated, and the technique may circumvent the need for histological analysis depending on the desired outcome. The ultrametric method differs from the traditional autodiametric method in that PVOs can be separated by ultrasonication and then enumerated, and ovarian homogeneity is not a mandatory requirement per se. The method is designed for determinate spawners but might be extended to include indeterminate spawners. |
format |
Text |
author |
Anderson, Kelli C. Alix, Maud Charitonidou, Katerina Thorsen, Anders Thorsheim, Grethe Ganias, Kostas Schmidt, Thassya C. dos Santos Kjesbu, Olav Sigurd |
author_facet |
Anderson, Kelli C. Alix, Maud Charitonidou, Katerina Thorsen, Anders Thorsheim, Grethe Ganias, Kostas Schmidt, Thassya C. dos Santos Kjesbu, Olav Sigurd |
author_sort |
Anderson, Kelli C. |
title |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_short |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_full |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_fullStr |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_full_unstemmed |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_sort |
development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
publisher |
Nature Publishing Group UK |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295772/ http://www.ncbi.nlm.nih.gov/pubmed/32541862 https://doi.org/10.1038/s41598-020-66601-w |
geographic |
Barents Sea |
geographic_facet |
Barents Sea |
genre |
atlantic cod Barents Sea |
genre_facet |
atlantic cod Barents Sea |
op_source |
Sci Rep |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295772/ http://www.ncbi.nlm.nih.gov/pubmed/32541862 http://dx.doi.org/10.1038/s41598-020-66601-w |
op_rights |
© The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
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https://doi.org/10.1038/s41598-020-66601-w |
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Scientific Reports |
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10 |
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1 |
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