Characterization of a New Mixture of Mono-Rhamnolipids Produced by Pseudomonas gessardii Isolated from Edmonson Point (Antarctica)
Rhamnolipids (RLs) are surface-active molecules mainly produced by Pseudomonas spp. Antarctica is one of the less explored places on Earth and bioprospecting for novel RL producer strains represents a promising strategy for the discovery of novel structures. In the present study, 34 cultivable bacte...
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ftpubmed:oai:pubmedcentral.nih.gov:7281774 2023-05-15T13:38:01+02:00 Characterization of a New Mixture of Mono-Rhamnolipids Produced by Pseudomonas gessardii Isolated from Edmonson Point (Antarctica) Buonocore, Carmine Tedesco, Pietro Vitale, Giovanni Andrea Palma Esposito, Fortunato Giugliano, Rosa Monti, Maria Chiara D’Auria, Maria Valeria de Pascale, Donatella 2020-05-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281774/ http://www.ncbi.nlm.nih.gov/pubmed/32443698 https://doi.org/10.3390/md18050269 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281774/ http://www.ncbi.nlm.nih.gov/pubmed/32443698 http://dx.doi.org/10.3390/md18050269 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Mar Drugs Article Text 2020 ftpubmed https://doi.org/10.3390/md18050269 2020-06-21T00:31:50Z Rhamnolipids (RLs) are surface-active molecules mainly produced by Pseudomonas spp. Antarctica is one of the less explored places on Earth and bioprospecting for novel RL producer strains represents a promising strategy for the discovery of novel structures. In the present study, 34 cultivable bacteria isolated from Edmonson Point Lake, Ross Sea, Antarctica were subjected to preliminary screening for the biosurfactant activity. The positive strains were identified by 16S rRNA gene sequencing and the produced RLs were characterized by liquid chromatography coupled to high resolution mass spectrometry (LC-HRESIMS) and liquid chromatography coupled with tandem spectrometry (LC-MS/MS), resulting in a new mixture of 17 different RL congeners, with six previously undescribed RLs. We explored the influence of the carbon source on the RL composition using 12 different raw materials, such as monosaccharides, polysaccharides and petroleum industry derivatives, reporting for the first time the production of RLs using, as sole carbon source, anthracene and benzene. Moreover, we investigated the antimicrobial potential of the RL mixture, towards a panel of both Gram-positive and Gram-negative pathogens, reporting very interesting results towards Listeria monocytogenes with a minimum inhibitory concentration (MIC) value of 3.13 µg/mL. Finally, we report for the first time the antimicrobial activity of RLs towards three strains of the emerging multidrug resistant Stenotrophomonas maltophilia with MIC values of 12.5 µg/mL. Text Antarc* Antarctica Ross Sea PubMed Central (PMC) Edmonson Point ENVELOPE(165.133,165.133,-74.333,-74.333) Ross Sea Marine Drugs 18 5 269 |
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Article Buonocore, Carmine Tedesco, Pietro Vitale, Giovanni Andrea Palma Esposito, Fortunato Giugliano, Rosa Monti, Maria Chiara D’Auria, Maria Valeria de Pascale, Donatella Characterization of a New Mixture of Mono-Rhamnolipids Produced by Pseudomonas gessardii Isolated from Edmonson Point (Antarctica) |
topic_facet |
Article |
description |
Rhamnolipids (RLs) are surface-active molecules mainly produced by Pseudomonas spp. Antarctica is one of the less explored places on Earth and bioprospecting for novel RL producer strains represents a promising strategy for the discovery of novel structures. In the present study, 34 cultivable bacteria isolated from Edmonson Point Lake, Ross Sea, Antarctica were subjected to preliminary screening for the biosurfactant activity. The positive strains were identified by 16S rRNA gene sequencing and the produced RLs were characterized by liquid chromatography coupled to high resolution mass spectrometry (LC-HRESIMS) and liquid chromatography coupled with tandem spectrometry (LC-MS/MS), resulting in a new mixture of 17 different RL congeners, with six previously undescribed RLs. We explored the influence of the carbon source on the RL composition using 12 different raw materials, such as monosaccharides, polysaccharides and petroleum industry derivatives, reporting for the first time the production of RLs using, as sole carbon source, anthracene and benzene. Moreover, we investigated the antimicrobial potential of the RL mixture, towards a panel of both Gram-positive and Gram-negative pathogens, reporting very interesting results towards Listeria monocytogenes with a minimum inhibitory concentration (MIC) value of 3.13 µg/mL. Finally, we report for the first time the antimicrobial activity of RLs towards three strains of the emerging multidrug resistant Stenotrophomonas maltophilia with MIC values of 12.5 µg/mL. |
format |
Text |
author |
Buonocore, Carmine Tedesco, Pietro Vitale, Giovanni Andrea Palma Esposito, Fortunato Giugliano, Rosa Monti, Maria Chiara D’Auria, Maria Valeria de Pascale, Donatella |
author_facet |
Buonocore, Carmine Tedesco, Pietro Vitale, Giovanni Andrea Palma Esposito, Fortunato Giugliano, Rosa Monti, Maria Chiara D’Auria, Maria Valeria de Pascale, Donatella |
author_sort |
Buonocore, Carmine |
title |
Characterization of a New Mixture of Mono-Rhamnolipids Produced by Pseudomonas gessardii Isolated from Edmonson Point (Antarctica) |
title_short |
Characterization of a New Mixture of Mono-Rhamnolipids Produced by Pseudomonas gessardii Isolated from Edmonson Point (Antarctica) |
title_full |
Characterization of a New Mixture of Mono-Rhamnolipids Produced by Pseudomonas gessardii Isolated from Edmonson Point (Antarctica) |
title_fullStr |
Characterization of a New Mixture of Mono-Rhamnolipids Produced by Pseudomonas gessardii Isolated from Edmonson Point (Antarctica) |
title_full_unstemmed |
Characterization of a New Mixture of Mono-Rhamnolipids Produced by Pseudomonas gessardii Isolated from Edmonson Point (Antarctica) |
title_sort |
characterization of a new mixture of mono-rhamnolipids produced by pseudomonas gessardii isolated from edmonson point (antarctica) |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281774/ http://www.ncbi.nlm.nih.gov/pubmed/32443698 https://doi.org/10.3390/md18050269 |
long_lat |
ENVELOPE(165.133,165.133,-74.333,-74.333) |
geographic |
Edmonson Point Ross Sea |
geographic_facet |
Edmonson Point Ross Sea |
genre |
Antarc* Antarctica Ross Sea |
genre_facet |
Antarc* Antarctica Ross Sea |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281774/ http://www.ncbi.nlm.nih.gov/pubmed/32443698 http://dx.doi.org/10.3390/md18050269 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/md18050269 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
5 |
container_start_page |
269 |
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1766100597894807552 |