Mechanisms of Bioactivities of Fucoidan from the Brown Seaweed Fucus vesiculosus L. of the Barents Sea
The aim of this study was to elucidate some mechanisms of radical scavenging and the anti-inflammatory, anti-hyperglycemic, and anti-coagulant bioactivities of high molecular weight fucoidan from Fucus vesiculosus in several in vitro models. Fucoidan has displayed potent 1, 1-diphenyl-2-picryl hydra...
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ftpubmed:oai:pubmedcentral.nih.gov:7281726 2023-05-15T15:39:09+02:00 Mechanisms of Bioactivities of Fucoidan from the Brown Seaweed Fucus vesiculosus L. of the Barents Sea Pozharitskaya, Olga N. Obluchinskaya, Ekaterina D. Shikov, Alexander N. 2020-05-22 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281726/ http://www.ncbi.nlm.nih.gov/pubmed/32456047 https://doi.org/10.3390/md18050275 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281726/ http://www.ncbi.nlm.nih.gov/pubmed/32456047 http://dx.doi.org/10.3390/md18050275 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Mar Drugs Article Text 2020 ftpubmed https://doi.org/10.3390/md18050275 2020-06-21T00:31:42Z The aim of this study was to elucidate some mechanisms of radical scavenging and the anti-inflammatory, anti-hyperglycemic, and anti-coagulant bioactivities of high molecular weight fucoidan from Fucus vesiculosus in several in vitro models. Fucoidan has displayed potent 1, 1-diphenyl-2-picryl hydrazil radical scavenging and reduction power activities. It significantly inhibits the cyclooxygenase-2 (COX-2) enzyme (IC(50) 4.3 μg mL(−1)) with a greater selectivity index (lg(IC(80) COX-2/IC(80)COX-1), −1.55) than the synthetic non-steroidal anti-inflammatory drug indomethacin (lg(IC(80) COX-2/IC(80)COX-1), −0.09). A concentration-dependent inhibition of hyaluronidase enzyme with an IC(50) of 2.9 μg mL(−1) was observed. Fucoidan attenuated the lipopolysaccharide-induced expression of mitogen-activated protein kinase p38. Our findings suggest that the inhibition of dipeptidyl peptidase-IV (DPP-IV) (IC(50) 1.11 μg mL(−1)) is one of the possible mechanisms involved in the anti-hyperglycemic activity of fucoidan. At a concentration of 3.2 μg mL(−1), fucoidan prolongs the activated partial thromboplastin time and thrombin time by 1.5-fold and 2.5-fold compared with a control, respectively. A significant increase of prothrombin time was observed after the concentration of fucoidan was increased above 80 μg mL(−1). This evidenced that fucoidan may have an effect on intrinsic/common pathways and little effect on the extrinsic mechanism. This study sheds light on the multiple pathways of the bioactivities of fucoidan. As far as we know, the inhibition of hyaluronidase and DPP-IV by high molecular fucoidan was studied for the first time in this work. Our results and literature data suggest that molecular weight, sulfate content, fucose content, and polyphenols may contribute to these activities. It seems that high molecular weight fucoidan has promising therapeutic applications in different pharmacological settings. Anti-oxidant, anti-inflammatory and anti-coagulant drugs have been used for the management of complications of ... Text Barents Sea PubMed Central (PMC) Barents Sea Marine Drugs 18 5 275 |
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Article Pozharitskaya, Olga N. Obluchinskaya, Ekaterina D. Shikov, Alexander N. Mechanisms of Bioactivities of Fucoidan from the Brown Seaweed Fucus vesiculosus L. of the Barents Sea |
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Article |
description |
The aim of this study was to elucidate some mechanisms of radical scavenging and the anti-inflammatory, anti-hyperglycemic, and anti-coagulant bioactivities of high molecular weight fucoidan from Fucus vesiculosus in several in vitro models. Fucoidan has displayed potent 1, 1-diphenyl-2-picryl hydrazil radical scavenging and reduction power activities. It significantly inhibits the cyclooxygenase-2 (COX-2) enzyme (IC(50) 4.3 μg mL(−1)) with a greater selectivity index (lg(IC(80) COX-2/IC(80)COX-1), −1.55) than the synthetic non-steroidal anti-inflammatory drug indomethacin (lg(IC(80) COX-2/IC(80)COX-1), −0.09). A concentration-dependent inhibition of hyaluronidase enzyme with an IC(50) of 2.9 μg mL(−1) was observed. Fucoidan attenuated the lipopolysaccharide-induced expression of mitogen-activated protein kinase p38. Our findings suggest that the inhibition of dipeptidyl peptidase-IV (DPP-IV) (IC(50) 1.11 μg mL(−1)) is one of the possible mechanisms involved in the anti-hyperglycemic activity of fucoidan. At a concentration of 3.2 μg mL(−1), fucoidan prolongs the activated partial thromboplastin time and thrombin time by 1.5-fold and 2.5-fold compared with a control, respectively. A significant increase of prothrombin time was observed after the concentration of fucoidan was increased above 80 μg mL(−1). This evidenced that fucoidan may have an effect on intrinsic/common pathways and little effect on the extrinsic mechanism. This study sheds light on the multiple pathways of the bioactivities of fucoidan. As far as we know, the inhibition of hyaluronidase and DPP-IV by high molecular fucoidan was studied for the first time in this work. Our results and literature data suggest that molecular weight, sulfate content, fucose content, and polyphenols may contribute to these activities. It seems that high molecular weight fucoidan has promising therapeutic applications in different pharmacological settings. Anti-oxidant, anti-inflammatory and anti-coagulant drugs have been used for the management of complications of ... |
format |
Text |
author |
Pozharitskaya, Olga N. Obluchinskaya, Ekaterina D. Shikov, Alexander N. |
author_facet |
Pozharitskaya, Olga N. Obluchinskaya, Ekaterina D. Shikov, Alexander N. |
author_sort |
Pozharitskaya, Olga N. |
title |
Mechanisms of Bioactivities of Fucoidan from the Brown Seaweed Fucus vesiculosus L. of the Barents Sea |
title_short |
Mechanisms of Bioactivities of Fucoidan from the Brown Seaweed Fucus vesiculosus L. of the Barents Sea |
title_full |
Mechanisms of Bioactivities of Fucoidan from the Brown Seaweed Fucus vesiculosus L. of the Barents Sea |
title_fullStr |
Mechanisms of Bioactivities of Fucoidan from the Brown Seaweed Fucus vesiculosus L. of the Barents Sea |
title_full_unstemmed |
Mechanisms of Bioactivities of Fucoidan from the Brown Seaweed Fucus vesiculosus L. of the Barents Sea |
title_sort |
mechanisms of bioactivities of fucoidan from the brown seaweed fucus vesiculosus l. of the barents sea |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281726/ http://www.ncbi.nlm.nih.gov/pubmed/32456047 https://doi.org/10.3390/md18050275 |
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Barents Sea |
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Barents Sea |
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Barents Sea |
genre_facet |
Barents Sea |
op_source |
Mar Drugs |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7281726/ http://www.ncbi.nlm.nih.gov/pubmed/32456047 http://dx.doi.org/10.3390/md18050275 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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https://doi.org/10.3390/md18050275 |
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Marine Drugs |
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18 |
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275 |
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