Characterization of Myxovirus resistance protein in birds showing different susceptibilities to highly pathogenic influenza virus
We compared the Mx expression and anti-viral function and the 3D structure of Mx protein in four species: chicken (Gallus gallus), whooper swan (Cygnus cygnus), jungle crow (Corvus macrorhynchos), and rock dove (Columba livia). We observed different mortalities associated with highly pathogenic avia...
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The Japanese Society of Veterinary Science
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ftpubmed:oai:pubmedcentral.nih.gov:7273593 2023-05-15T15:59:49+02:00 Characterization of Myxovirus resistance protein in birds showing different susceptibilities to highly pathogenic influenza virus HAGIWARA, Katsuro NAKAYA, Takaaki ONUMA, Manabu 2020-03-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273593/ http://www.ncbi.nlm.nih.gov/pubmed/32173692 https://doi.org/10.1292/jvms.19-0408 en eng The Japanese Society of Veterinary Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273593/ http://www.ncbi.nlm.nih.gov/pubmed/32173692 http://dx.doi.org/10.1292/jvms.19-0408 ©2020 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) CC-BY-NC-ND J Vet Med Sci Wildlife Science Text 2020 ftpubmed https://doi.org/10.1292/jvms.19-0408 2020-06-14T00:36:22Z We compared the Mx expression and anti-viral function and the 3D structure of Mx protein in four species: chicken (Gallus gallus), whooper swan (Cygnus cygnus), jungle crow (Corvus macrorhynchos), and rock dove (Columba livia). We observed different mortalities associated with highly pathogenic avian influenza virus (HPAIV) infection to understand the relationship between Mx function as an immune response factor and HPAIV proliferation in bird cells. Different levels of Mx were observed among the different bird species after virus infection. Strong Mx expression was confirmed in the rock dove and whooper swan 6 hr after viral infection. The lowest virus copy numbers were observed in rock dove. The virus infectivity was significantly reduced in the BALB/3T3 cells expressing rock dove and jungle crow Mx. These results suggested that high Mx expression and significant Mx-induced anti-viral effects might result in the rock dove primary cells having the lowest virus copy number. Comparison of the expected 3D structure of Mx protein in all four bird species demonstrated that the structure of loop L4 varied among the investigated species. It was reported that differences in amino acid sequence in loop L4 affect antiviral activity in human and mouse cells, and a significant anti-viral effect was observed in the rock dove Mx. Thus, the amino acid sequence of loop L4 in rock dove might represent relatively high anti-viral activity. Text Cygnus cygnus Whooper Swan PubMed Central (PMC) Journal of Veterinary Medical Science 82 5 619 625 |
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English |
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Wildlife Science |
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Wildlife Science HAGIWARA, Katsuro NAKAYA, Takaaki ONUMA, Manabu Characterization of Myxovirus resistance protein in birds showing different susceptibilities to highly pathogenic influenza virus |
topic_facet |
Wildlife Science |
description |
We compared the Mx expression and anti-viral function and the 3D structure of Mx protein in four species: chicken (Gallus gallus), whooper swan (Cygnus cygnus), jungle crow (Corvus macrorhynchos), and rock dove (Columba livia). We observed different mortalities associated with highly pathogenic avian influenza virus (HPAIV) infection to understand the relationship between Mx function as an immune response factor and HPAIV proliferation in bird cells. Different levels of Mx were observed among the different bird species after virus infection. Strong Mx expression was confirmed in the rock dove and whooper swan 6 hr after viral infection. The lowest virus copy numbers were observed in rock dove. The virus infectivity was significantly reduced in the BALB/3T3 cells expressing rock dove and jungle crow Mx. These results suggested that high Mx expression and significant Mx-induced anti-viral effects might result in the rock dove primary cells having the lowest virus copy number. Comparison of the expected 3D structure of Mx protein in all four bird species demonstrated that the structure of loop L4 varied among the investigated species. It was reported that differences in amino acid sequence in loop L4 affect antiviral activity in human and mouse cells, and a significant anti-viral effect was observed in the rock dove Mx. Thus, the amino acid sequence of loop L4 in rock dove might represent relatively high anti-viral activity. |
format |
Text |
author |
HAGIWARA, Katsuro NAKAYA, Takaaki ONUMA, Manabu |
author_facet |
HAGIWARA, Katsuro NAKAYA, Takaaki ONUMA, Manabu |
author_sort |
HAGIWARA, Katsuro |
title |
Characterization of Myxovirus resistance protein in birds showing different susceptibilities to highly pathogenic influenza virus |
title_short |
Characterization of Myxovirus resistance protein in birds showing different susceptibilities to highly pathogenic influenza virus |
title_full |
Characterization of Myxovirus resistance protein in birds showing different susceptibilities to highly pathogenic influenza virus |
title_fullStr |
Characterization of Myxovirus resistance protein in birds showing different susceptibilities to highly pathogenic influenza virus |
title_full_unstemmed |
Characterization of Myxovirus resistance protein in birds showing different susceptibilities to highly pathogenic influenza virus |
title_sort |
characterization of myxovirus resistance protein in birds showing different susceptibilities to highly pathogenic influenza virus |
publisher |
The Japanese Society of Veterinary Science |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273593/ http://www.ncbi.nlm.nih.gov/pubmed/32173692 https://doi.org/10.1292/jvms.19-0408 |
genre |
Cygnus cygnus Whooper Swan |
genre_facet |
Cygnus cygnus Whooper Swan |
op_source |
J Vet Med Sci |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273593/ http://www.ncbi.nlm.nih.gov/pubmed/32173692 http://dx.doi.org/10.1292/jvms.19-0408 |
op_rights |
©2020 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
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CC-BY-NC-ND |
op_doi |
https://doi.org/10.1292/jvms.19-0408 |
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Journal of Veterinary Medical Science |
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82 |
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5 |
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619 |
op_container_end_page |
625 |
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1766395726283145216 |