Specific adaptations are selected in opposite sun exposed Antarctic cryptoendolithic communities as revealed by untargeted metabolomics
Antarctic cryptoendolithic communities are self-supporting borderline ecosystems spreading across the extreme conditions of the Antarctic desert and represent the predominant life-form in the ice-free areas of McMurdo Dry Valleys, accounted as the closest terrestrial Martian analogue. Components of...
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ftpubmed:oai:pubmedcentral.nih.gov:7253227 2023-05-15T14:00:03+02:00 Specific adaptations are selected in opposite sun exposed Antarctic cryptoendolithic communities as revealed by untargeted metabolomics Coleine, Claudia Gevi, Federica Fanelli, Giuseppina Onofri, Silvano Timperio, Anna Maria Selbmann, Laura 2020-05-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253227/ http://www.ncbi.nlm.nih.gov/pubmed/32460306 https://doi.org/10.1371/journal.pone.0233805 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253227/ http://www.ncbi.nlm.nih.gov/pubmed/32460306 http://dx.doi.org/10.1371/journal.pone.0233805 © 2020 Coleine et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY PLoS One Research Article Text 2020 ftpubmed https://doi.org/10.1371/journal.pone.0233805 2020-06-14T00:22:50Z Antarctic cryptoendolithic communities are self-supporting borderline ecosystems spreading across the extreme conditions of the Antarctic desert and represent the predominant life-form in the ice-free areas of McMurdo Dry Valleys, accounted as the closest terrestrial Martian analogue. Components of these communities are highly adapted extremophiles and extreme-tolerant microorganisms, among the most resistant known to date. Recently, studies investigated biodiversity and community composition in these ecosystems but the metabolic activity of the metacommunity has never been investigated. Using an untargeted metabolomics, we explored stress-response of communities spreading in two sites of the same location, subjected to increasing environmental pressure due to opposite sun exposure, accounted as main factor influencing the diversity and composition of these ecosystems. Overall, 331 altered metabolites (206 and 125 unique for north and south, respectively), distinguished the two differently exposed communities. We also selected 10 metabolites and performed two-stage Receiver Operating Characteristic (ROC) analysis to test them as potential biomarkers. We further focused on melanin and allantoin as protective substances; their concentration was highly different in the community in the shadow or in the sun. These results clearly indicate that opposite insolation selected organisms in the communities with different adaptation strategies in terms of key metabolites produced. Text Antarc* Antarctic McMurdo Dry Valleys PubMed Central (PMC) Antarctic McMurdo Dry Valleys The Antarctic PLOS ONE 15 5 e0233805 |
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Research Article Coleine, Claudia Gevi, Federica Fanelli, Giuseppina Onofri, Silvano Timperio, Anna Maria Selbmann, Laura Specific adaptations are selected in opposite sun exposed Antarctic cryptoendolithic communities as revealed by untargeted metabolomics |
topic_facet |
Research Article |
description |
Antarctic cryptoendolithic communities are self-supporting borderline ecosystems spreading across the extreme conditions of the Antarctic desert and represent the predominant life-form in the ice-free areas of McMurdo Dry Valleys, accounted as the closest terrestrial Martian analogue. Components of these communities are highly adapted extremophiles and extreme-tolerant microorganisms, among the most resistant known to date. Recently, studies investigated biodiversity and community composition in these ecosystems but the metabolic activity of the metacommunity has never been investigated. Using an untargeted metabolomics, we explored stress-response of communities spreading in two sites of the same location, subjected to increasing environmental pressure due to opposite sun exposure, accounted as main factor influencing the diversity and composition of these ecosystems. Overall, 331 altered metabolites (206 and 125 unique for north and south, respectively), distinguished the two differently exposed communities. We also selected 10 metabolites and performed two-stage Receiver Operating Characteristic (ROC) analysis to test them as potential biomarkers. We further focused on melanin and allantoin as protective substances; their concentration was highly different in the community in the shadow or in the sun. These results clearly indicate that opposite insolation selected organisms in the communities with different adaptation strategies in terms of key metabolites produced. |
format |
Text |
author |
Coleine, Claudia Gevi, Federica Fanelli, Giuseppina Onofri, Silvano Timperio, Anna Maria Selbmann, Laura |
author_facet |
Coleine, Claudia Gevi, Federica Fanelli, Giuseppina Onofri, Silvano Timperio, Anna Maria Selbmann, Laura |
author_sort |
Coleine, Claudia |
title |
Specific adaptations are selected in opposite sun exposed Antarctic cryptoendolithic communities as revealed by untargeted metabolomics |
title_short |
Specific adaptations are selected in opposite sun exposed Antarctic cryptoendolithic communities as revealed by untargeted metabolomics |
title_full |
Specific adaptations are selected in opposite sun exposed Antarctic cryptoendolithic communities as revealed by untargeted metabolomics |
title_fullStr |
Specific adaptations are selected in opposite sun exposed Antarctic cryptoendolithic communities as revealed by untargeted metabolomics |
title_full_unstemmed |
Specific adaptations are selected in opposite sun exposed Antarctic cryptoendolithic communities as revealed by untargeted metabolomics |
title_sort |
specific adaptations are selected in opposite sun exposed antarctic cryptoendolithic communities as revealed by untargeted metabolomics |
publisher |
Public Library of Science |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253227/ http://www.ncbi.nlm.nih.gov/pubmed/32460306 https://doi.org/10.1371/journal.pone.0233805 |
geographic |
Antarctic McMurdo Dry Valleys The Antarctic |
geographic_facet |
Antarctic McMurdo Dry Valleys The Antarctic |
genre |
Antarc* Antarctic McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic McMurdo Dry Valleys |
op_source |
PLoS One |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253227/ http://www.ncbi.nlm.nih.gov/pubmed/32460306 http://dx.doi.org/10.1371/journal.pone.0233805 |
op_rights |
© 2020 Coleine et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0233805 |
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PLOS ONE |
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15 |
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5 |
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e0233805 |
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1766269028728307712 |