Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression

• Published in: Frontiers in Microbiology
• Main Authors: Perfumo, Amedea, Freiherr von Sass, Georg Johannes, Nordmann, Eva-Lena, Budisa, Nediljko, Wagner, Dirk
• Format: Text
• Language: English
• Published: Frontiers Media S.A. 2020
• Subjects: Microbiology; Antarctic; Triton; Antarc*
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247812/; https://doi.org/10.3389/fmicb.2020.00881

Following a screening of Antarctic glacier forefield-bacteria for novel cold-active enzymes, a psychrophilic strain Psychrobacter sp. 94-6PB was selected for further characterization of enzymatic activities. The strain produced lipases and proteases in the temperature range of 4–18°C. The coding seq...