Sonohydrolysis using an enzymatic cocktail in the preparation of free fatty acid

• Published in: 3 Biotech
• Main Authors: Souza, José E. S., Monteiro, Rodolpho R. C., Rocha, Thales G., Moreira, Katerine S., Cavalcante, Francisco T. T., de Sousa Braz, Ana K., de Souza, Maria C. M., dos Santos, José C. S.
• Format: Text
• Language: English
• Published: Springer International Publishing 2020
• Subjects: Original Article; Antarc*; Antarctica
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229052/; http://www.ncbi.nlm.nih.gov/pubmed/32426206; https://doi.org/10.1007/s13205-020-02227-z

In this work, the concept of lipase cocktail has been proposed in the ultrasound-assisted hydrolysis of coconut oil. Lipase from Thermomyces lanuginosus (TLL), lipase from Rhizomucor miehei (RML), and lipase B from Candida antarctica (CALB) were evaluated as biocatalysts in different combinations. The best conversion (33.66%) was achieved using only RML; however, the best lipase cocktail (75% RML and 25% CALB) proposed by the triangular response surface was used to achieve higher conversions. At the best lipase cocktail, reaction parameters [temperature, biocatalyst content and molar ratio (water/oil)] were optimized by a Central Composite Design, allowing to obtain more than 98% of conversion in the hydrolysis of coconut oil in 3 h of incubation at 37 kHz, 300 W and 45 °C by using 20% of the lipase cocktail (w/w) and a molar ratio of 7.5:1 (water/oil). The lipase cocktail retained about 50% of its initial activity after three consecutive cycles of hydrolysis. To the authors’ knowledge, up to date, this communication is the first report in the literature for the ultrasound-assisted hydrolysis of coconut oil catalyzed by a cocktail of lipases. Under ultrasound irradiation, the concept of lipase cocktail was successfully applied, and this strategy could be useful for the other types of reactions using heterogeneous substrates.