Extreme Viral Partitioning in a Marine-Derived High Arctic Lake
High-latitude, perennially stratified (meromictic) lakes are likely to be especially vulnerable to climate warming because of the importance of ice in maintaining their water column structure and associated distribution of microbial communities. This study aimed to characterize viral abundance, dive...
Published in: | mSphere |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
American Society for Microbiology
2020
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227771/ http://www.ncbi.nlm.nih.gov/pubmed/32404515 https://doi.org/10.1128/mSphere.00334-20 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:7227771 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:7227771 2023-05-15T13:49:32+02:00 Extreme Viral Partitioning in a Marine-Derived High Arctic Lake Labbé, Myriam Girard, Catherine Vincent, Warwick F. Culley, Alexander I. 2020-05-13 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227771/ http://www.ncbi.nlm.nih.gov/pubmed/32404515 https://doi.org/10.1128/mSphere.00334-20 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227771/ http://www.ncbi.nlm.nih.gov/pubmed/32404515 http://dx.doi.org/10.1128/mSphere.00334-20 Copyright © 2020 Labbé et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . CC-BY mSphere Research Article Text 2020 ftpubmed https://doi.org/10.1128/mSphere.00334-20 2020-05-24T00:35:12Z High-latitude, perennially stratified (meromictic) lakes are likely to be especially vulnerable to climate warming because of the importance of ice in maintaining their water column structure and associated distribution of microbial communities. This study aimed to characterize viral abundance, diversity, and distribution in a meromictic lake of marine origin on the far northern coast of Ellesmere Island, in the Canadian High Arctic. We collected triplicate samples for double-stranded DNA (dsDNA) viromics from five depths that encompassed the major features of the lake, as determined by limnological profiling of the water column. Viral abundance and virus-to-prokaryote ratios were highest at greater depths, while bacterial and cyanobacterial counts were greatest in the surface waters. The viral communities from each zone of the lake defined by salinity, temperature, and dissolved oxygen concentrations were markedly distinct, suggesting that there was little exchange of viral types among lake strata. Ten viral assembled genomes were obtained from our libraries, and these also segregated with depth. This well-defined structure of viral communities was consistent with that of potential hosts. Viruses from the monimolimnion, a deep layer of ancient Arctic Ocean seawater, were more diverse and relatively abundant, with few similarities to available viral sequences. The Lake A viral communities also differed from published records from the Arctic Ocean and meromictic Ace Lake in Antarctica. This first characterization of viral diversity from this sentinel environment underscores the microbial richness and complexity of an ecosystem type that is increasingly exposed to major perturbations in the fast-changing Arctic. IMPORTANCE The Arctic is warming at an accelerating pace, and the rise in temperature has increasing impacts on the Arctic biome. Lakes are integrators of their surroundings and thus excellent sentinels of environmental change. Despite their importance in the regulation of key microbial processes, viruses ... Text Antarc* Antarctica Arctic Arctic Ocean Ellesmere Island PubMed Central (PMC) Ace Lake ENVELOPE(78.188,78.188,-68.472,-68.472) Arctic Arctic Lake ENVELOPE(-130.826,-130.826,57.231,57.231) Arctic Ocean Ellesmere Island mSphere 5 3 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Labbé, Myriam Girard, Catherine Vincent, Warwick F. Culley, Alexander I. Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
topic_facet |
Research Article |
description |
High-latitude, perennially stratified (meromictic) lakes are likely to be especially vulnerable to climate warming because of the importance of ice in maintaining their water column structure and associated distribution of microbial communities. This study aimed to characterize viral abundance, diversity, and distribution in a meromictic lake of marine origin on the far northern coast of Ellesmere Island, in the Canadian High Arctic. We collected triplicate samples for double-stranded DNA (dsDNA) viromics from five depths that encompassed the major features of the lake, as determined by limnological profiling of the water column. Viral abundance and virus-to-prokaryote ratios were highest at greater depths, while bacterial and cyanobacterial counts were greatest in the surface waters. The viral communities from each zone of the lake defined by salinity, temperature, and dissolved oxygen concentrations were markedly distinct, suggesting that there was little exchange of viral types among lake strata. Ten viral assembled genomes were obtained from our libraries, and these also segregated with depth. This well-defined structure of viral communities was consistent with that of potential hosts. Viruses from the monimolimnion, a deep layer of ancient Arctic Ocean seawater, were more diverse and relatively abundant, with few similarities to available viral sequences. The Lake A viral communities also differed from published records from the Arctic Ocean and meromictic Ace Lake in Antarctica. This first characterization of viral diversity from this sentinel environment underscores the microbial richness and complexity of an ecosystem type that is increasingly exposed to major perturbations in the fast-changing Arctic. IMPORTANCE The Arctic is warming at an accelerating pace, and the rise in temperature has increasing impacts on the Arctic biome. Lakes are integrators of their surroundings and thus excellent sentinels of environmental change. Despite their importance in the regulation of key microbial processes, viruses ... |
format |
Text |
author |
Labbé, Myriam Girard, Catherine Vincent, Warwick F. Culley, Alexander I. |
author_facet |
Labbé, Myriam Girard, Catherine Vincent, Warwick F. Culley, Alexander I. |
author_sort |
Labbé, Myriam |
title |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_short |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_full |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_fullStr |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_full_unstemmed |
Extreme Viral Partitioning in a Marine-Derived High Arctic Lake |
title_sort |
extreme viral partitioning in a marine-derived high arctic lake |
publisher |
American Society for Microbiology |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227771/ http://www.ncbi.nlm.nih.gov/pubmed/32404515 https://doi.org/10.1128/mSphere.00334-20 |
long_lat |
ENVELOPE(78.188,78.188,-68.472,-68.472) ENVELOPE(-130.826,-130.826,57.231,57.231) |
geographic |
Ace Lake Arctic Arctic Lake Arctic Ocean Ellesmere Island |
geographic_facet |
Ace Lake Arctic Arctic Lake Arctic Ocean Ellesmere Island |
genre |
Antarc* Antarctica Arctic Arctic Ocean Ellesmere Island |
genre_facet |
Antarc* Antarctica Arctic Arctic Ocean Ellesmere Island |
op_source |
mSphere |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227771/ http://www.ncbi.nlm.nih.gov/pubmed/32404515 http://dx.doi.org/10.1128/mSphere.00334-20 |
op_rights |
Copyright © 2020 Labbé et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1128/mSphere.00334-20 |
container_title |
mSphere |
container_volume |
5 |
container_issue |
3 |
_version_ |
1766251473219354624 |