Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)
SIMPLE SUMMARY: The process of semen cryopreservation can have multiple advantages in an ex situ conservation program. However, there is a necessity to adapt the protocol to the specificity of each species. With that in mind, we aimed to optimize the sperm freezing/thawing process and study the effe...
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ftpubmed:oai:pubmedcentral.nih.gov:7222757 2023-05-15T16:10:00+02:00 Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) Cardoso, Beatriz Sánchez-Ajofrín, Irene Castaño, Cristina García-Álvarez, Olga Esteso, Milagros Cristina Maroto-Morales, Alejandro Iniesta-Cuerda, María Garde, José Julián Santiago-Moreno, Julián Soler, Ana Josefa 2020-04-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222757/ http://www.ncbi.nlm.nih.gov/pubmed/32316152 https://doi.org/10.3390/ani10040691 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222757/ http://www.ncbi.nlm.nih.gov/pubmed/32316152 http://dx.doi.org/10.3390/ani10040691 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Animals (Basel) Article Text 2020 ftpubmed https://doi.org/10.3390/ani10040691 2020-05-24T00:30:07Z SIMPLE SUMMARY: The process of semen cryopreservation can have multiple advantages in an ex situ conservation program. However, there is a necessity to adapt the protocol to the specificity of each species. With that in mind, we aimed to optimize the sperm freezing/thawing process and study the effect of different cryoprotectants in the peregrine falcon. ABSTRACT: Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs. ultrarapid freezing in pellets), thawing conditions (37 °C for 30 s vs. 5 °C for 1 min), type of cryoprotectant (DMA vs. DMSO), and concentration of DMSO (4% vs. 8%). Results show that slow cryopreservation in straws yielded greater percentages (p < 0.05) of motile spermatozoa (22.5% ± 4.4% vs. 0.0% ± 4.1%), viable spermatozoa with intact acrosomes (84.6% ± 4.3% vs. 77.4% ± 4.3%), and spermatozoa with active mitochondria (41.0% ± 6.7% vs.12.8% ± 6.7%), compared with those obtained by the ultrarapid freezing in pellets. However, no differences were found between different thawing conditions. Moreover, all sperm motility parameters were greater (p < 0.05) when DMSO was used during freezing compared with DMA, although the use of 3% and 8% DMSO produced similar results. In conclusion, these results represent important progress in the study of falcon semen cryopreservation protocol, highlighting the crucial steps of the process and the most suitable conditions. Text Falco peregrinus peregrine falcon PubMed Central (PMC) Animals 10 4 691 |
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Article Cardoso, Beatriz Sánchez-Ajofrín, Irene Castaño, Cristina García-Álvarez, Olga Esteso, Milagros Cristina Maroto-Morales, Alejandro Iniesta-Cuerda, María Garde, José Julián Santiago-Moreno, Julián Soler, Ana Josefa Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
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SIMPLE SUMMARY: The process of semen cryopreservation can have multiple advantages in an ex situ conservation program. However, there is a necessity to adapt the protocol to the specificity of each species. With that in mind, we aimed to optimize the sperm freezing/thawing process and study the effect of different cryoprotectants in the peregrine falcon. ABSTRACT: Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs. ultrarapid freezing in pellets), thawing conditions (37 °C for 30 s vs. 5 °C for 1 min), type of cryoprotectant (DMA vs. DMSO), and concentration of DMSO (4% vs. 8%). Results show that slow cryopreservation in straws yielded greater percentages (p < 0.05) of motile spermatozoa (22.5% ± 4.4% vs. 0.0% ± 4.1%), viable spermatozoa with intact acrosomes (84.6% ± 4.3% vs. 77.4% ± 4.3%), and spermatozoa with active mitochondria (41.0% ± 6.7% vs.12.8% ± 6.7%), compared with those obtained by the ultrarapid freezing in pellets. However, no differences were found between different thawing conditions. Moreover, all sperm motility parameters were greater (p < 0.05) when DMSO was used during freezing compared with DMA, although the use of 3% and 8% DMSO produced similar results. In conclusion, these results represent important progress in the study of falcon semen cryopreservation protocol, highlighting the crucial steps of the process and the most suitable conditions. |
format |
Text |
author |
Cardoso, Beatriz Sánchez-Ajofrín, Irene Castaño, Cristina García-Álvarez, Olga Esteso, Milagros Cristina Maroto-Morales, Alejandro Iniesta-Cuerda, María Garde, José Julián Santiago-Moreno, Julián Soler, Ana Josefa |
author_facet |
Cardoso, Beatriz Sánchez-Ajofrín, Irene Castaño, Cristina García-Álvarez, Olga Esteso, Milagros Cristina Maroto-Morales, Alejandro Iniesta-Cuerda, María Garde, José Julián Santiago-Moreno, Julián Soler, Ana Josefa |
author_sort |
Cardoso, Beatriz |
title |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_short |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_full |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_fullStr |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_full_unstemmed |
Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus) |
title_sort |
optimization of sperm cryopreservation protocol for peregrine falcon (falco peregrinus) |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222757/ http://www.ncbi.nlm.nih.gov/pubmed/32316152 https://doi.org/10.3390/ani10040691 |
genre |
Falco peregrinus peregrine falcon |
genre_facet |
Falco peregrinus peregrine falcon |
op_source |
Animals (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222757/ http://www.ncbi.nlm.nih.gov/pubmed/32316152 http://dx.doi.org/10.3390/ani10040691 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/ani10040691 |
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