Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature
In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtain...
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ftpubmed:oai:pubmedcentral.nih.gov:7222407 2023-05-15T13:47:48+02:00 Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature Chatzidimitriou, Evangelia Bisaccia, Paola Corrà, Francesca Bonato, Marco Irato, Paola Manuto, Laura Toppo, Stefano Bakiu, Rigers Santovito, Gianfranco 2020-04-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222407/ http://www.ncbi.nlm.nih.gov/pubmed/32316382 https://doi.org/10.3390/antiox9040325 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222407/ http://www.ncbi.nlm.nih.gov/pubmed/32316382 http://dx.doi.org/10.3390/antiox9040325 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Antioxidants (Basel) Article Text 2020 ftpubmed https://doi.org/10.3390/antiox9040325 2020-05-31T00:17:13Z In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approaches and compared with Cu,Zn SODs from other organisms. Multiple sequence alignment using the amino acid sequence revealed that Cu,Zn SOD showed considerable sequence similarity with its orthologues from various vertebrate species, but also some specific substitutions directly linked to cold adaptation. Phylogenetic analyses presented the monophyletic status of Antartic Teleostei among the Perciformes, confirming the erratic differentiation of these proteins and concurring with the theory of the “unclock-like” behavior of Cu,Zn SOD evolution. Expression of C. hamatus Cu,Zn SOD at both the mRNA and protein levels were analyzed in various tissues, highlighting the regulation of gene expression related to environmental stress conditions and also animal physiology. The data presented are the first on the antioxidant enzymes of a fish belonging to the Channichthyidae family and represent an important starting point in understanding the antioxidant systems of these organisms that are subject to constant risk of oxidative stress. Text Antarc* Antarctic antartic* Icefish Ross Sea PubMed Central (PMC) Antarctic Ross Sea The Antarctic Antioxidants 9 4 325 |
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Article Chatzidimitriou, Evangelia Bisaccia, Paola Corrà, Francesca Bonato, Marco Irato, Paola Manuto, Laura Toppo, Stefano Bakiu, Rigers Santovito, Gianfranco Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
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Article |
description |
In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approaches and compared with Cu,Zn SODs from other organisms. Multiple sequence alignment using the amino acid sequence revealed that Cu,Zn SOD showed considerable sequence similarity with its orthologues from various vertebrate species, but also some specific substitutions directly linked to cold adaptation. Phylogenetic analyses presented the monophyletic status of Antartic Teleostei among the Perciformes, confirming the erratic differentiation of these proteins and concurring with the theory of the “unclock-like” behavior of Cu,Zn SOD evolution. Expression of C. hamatus Cu,Zn SOD at both the mRNA and protein levels were analyzed in various tissues, highlighting the regulation of gene expression related to environmental stress conditions and also animal physiology. The data presented are the first on the antioxidant enzymes of a fish belonging to the Channichthyidae family and represent an important starting point in understanding the antioxidant systems of these organisms that are subject to constant risk of oxidative stress. |
format |
Text |
author |
Chatzidimitriou, Evangelia Bisaccia, Paola Corrà, Francesca Bonato, Marco Irato, Paola Manuto, Laura Toppo, Stefano Bakiu, Rigers Santovito, Gianfranco |
author_facet |
Chatzidimitriou, Evangelia Bisaccia, Paola Corrà, Francesca Bonato, Marco Irato, Paola Manuto, Laura Toppo, Stefano Bakiu, Rigers Santovito, Gianfranco |
author_sort |
Chatzidimitriou, Evangelia |
title |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_short |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_full |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_fullStr |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_full_unstemmed |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_sort |
copper/zinc superoxide dismutase from the crocodile icefish chionodraco hamatus: antioxidant defense at constant sub-zero temperature |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222407/ http://www.ncbi.nlm.nih.gov/pubmed/32316382 https://doi.org/10.3390/antiox9040325 |
geographic |
Antarctic Ross Sea The Antarctic |
geographic_facet |
Antarctic Ross Sea The Antarctic |
genre |
Antarc* Antarctic antartic* Icefish Ross Sea |
genre_facet |
Antarc* Antarctic antartic* Icefish Ross Sea |
op_source |
Antioxidants (Basel) |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222407/ http://www.ncbi.nlm.nih.gov/pubmed/32316382 http://dx.doi.org/10.3390/antiox9040325 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/antiox9040325 |
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Antioxidants |
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9 |
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325 |
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1766247890310660096 |