Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy
This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax d...
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ftpubmed:oai:pubmedcentral.nih.gov:7221741 2023-05-15T15:30:38+02:00 Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy Shumilina, Elena Ciampa, Alessandra Ytrestøyl, Trine Dikiy, Alexander 2020-04-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221741/ http://www.ncbi.nlm.nih.gov/pubmed/32290609 https://doi.org/10.3390/molecules25081769 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221741/ http://www.ncbi.nlm.nih.gov/pubmed/32290609 http://dx.doi.org/10.3390/molecules25081769 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Molecules Article Text 2020 ftpubmed https://doi.org/10.3390/molecules25081769 2020-05-24T00:29:08Z This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the (13)C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and (13)C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra. Text Atlantic salmon PubMed Central (PMC) Molecules 25 8 1769 |
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Article Shumilina, Elena Ciampa, Alessandra Ytrestøyl, Trine Dikiy, Alexander Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
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Article |
description |
This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the (13)C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and (13)C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra. |
format |
Text |
author |
Shumilina, Elena Ciampa, Alessandra Ytrestøyl, Trine Dikiy, Alexander |
author_facet |
Shumilina, Elena Ciampa, Alessandra Ytrestøyl, Trine Dikiy, Alexander |
author_sort |
Shumilina, Elena |
title |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_short |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_full |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_fullStr |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_full_unstemmed |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_sort |
assessment of astaxanthin accumulation in hepatocytes of atlantic salmon fed different diets using nmr spectroscopy |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221741/ http://www.ncbi.nlm.nih.gov/pubmed/32290609 https://doi.org/10.3390/molecules25081769 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Molecules |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221741/ http://www.ncbi.nlm.nih.gov/pubmed/32290609 http://dx.doi.org/10.3390/molecules25081769 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/molecules25081769 |
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Molecules |
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25 |
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8 |
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1769 |
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1766361094301941760 |