Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses
The Antarctic krill, Euphausia superba, is a Southern Ocean endemic species of proven ecological importance to the region. In the context of predicted global warming, it is particularly important to understand how classic biomarkers of heat stress function in this species. In this respect, Hsp70s ar...
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ftpubmed:oai:pubmedcentral.nih.gov:7192979 2023-05-15T13:58:32+02:00 Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses Toullec, Jean-Yves Cascella, Kévin Ruault, Stéphanie Geffroy, Alexandre Lorieux, David Montagné, Nicolas Ollivaux, Céline Lee, Chi-Ying 2020-03-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192979/ http://www.ncbi.nlm.nih.gov/pubmed/32215845 https://doi.org/10.1007/s12192-020-01103-2 en eng Springer Netherlands http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192979/ http://www.ncbi.nlm.nih.gov/pubmed/32215845 http://dx.doi.org/10.1007/s12192-020-01103-2 © Cell Stress Society International 2020 Cell Stress Chaperones Original Paper Text 2020 ftpubmed https://doi.org/10.1007/s12192-020-01103-2 2020-11-08T01:31:59Z The Antarctic krill, Euphausia superba, is a Southern Ocean endemic species of proven ecological importance to the region. In the context of predicted global warming, it is particularly important to understand how classic biomarkers of heat stress function in this species. In this respect, Hsp70s are acknowledged as good candidates. However, previous studies of expression kinetics have not been able to demonstrate significant upregulation of these genes in response to heat shocks at 3 °C and 6 °C for 3 and 6 h. The current work complements these previous results and broadens the prospects for the use of Hsp70s as a relevant marker of thermal shock in this krill species. New experiments demonstrate that induction of Hsp70 isoforms was not detected during exposure to heat shock, but increased expression was observed after several hours of recovery. To complete the analysis of the expression kinetics of the different isoforms, experiments were carried out over short time scales (1 and 2 h at 3 °C and 6 °C) as well as at higher temperatures (9 °C, 12 °C, and 15 °C for 3 h), without any significant response. A 6-week monitoring of animals at 3 °C showed that the time factor is decisive in the establishment of the response. CT(max) experiments with incremental times of 1 °C per day or 1 °C every 3 days have shown a particularly high resilience of the animals. The demonstration of the abundance of Hsp70s present before thermal stress in various species of krill, as well as in specimens of E. superba of various origins, showed that the delay in the response in expression could be related to the high constitutive levels of Hsp70 available before the stress experiments. The alternative labelling of the two main isoforms of Hsp70 according to the origin of the animals allowed hypotheses to be put forward on the functioning of thermoregulation in Antarctic krill as well as ice krill. Text Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean PubMed Central (PMC) Antarctic Southern Ocean The Antarctic Cell Stress and Chaperones 25 3 519 531 |
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Original Paper |
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Original Paper Toullec, Jean-Yves Cascella, Kévin Ruault, Stéphanie Geffroy, Alexandre Lorieux, David Montagné, Nicolas Ollivaux, Céline Lee, Chi-Ying Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
topic_facet |
Original Paper |
description |
The Antarctic krill, Euphausia superba, is a Southern Ocean endemic species of proven ecological importance to the region. In the context of predicted global warming, it is particularly important to understand how classic biomarkers of heat stress function in this species. In this respect, Hsp70s are acknowledged as good candidates. However, previous studies of expression kinetics have not been able to demonstrate significant upregulation of these genes in response to heat shocks at 3 °C and 6 °C for 3 and 6 h. The current work complements these previous results and broadens the prospects for the use of Hsp70s as a relevant marker of thermal shock in this krill species. New experiments demonstrate that induction of Hsp70 isoforms was not detected during exposure to heat shock, but increased expression was observed after several hours of recovery. To complete the analysis of the expression kinetics of the different isoforms, experiments were carried out over short time scales (1 and 2 h at 3 °C and 6 °C) as well as at higher temperatures (9 °C, 12 °C, and 15 °C for 3 h), without any significant response. A 6-week monitoring of animals at 3 °C showed that the time factor is decisive in the establishment of the response. CT(max) experiments with incremental times of 1 °C per day or 1 °C every 3 days have shown a particularly high resilience of the animals. The demonstration of the abundance of Hsp70s present before thermal stress in various species of krill, as well as in specimens of E. superba of various origins, showed that the delay in the response in expression could be related to the high constitutive levels of Hsp70 available before the stress experiments. The alternative labelling of the two main isoforms of Hsp70 according to the origin of the animals allowed hypotheses to be put forward on the functioning of thermoregulation in Antarctic krill as well as ice krill. |
format |
Text |
author |
Toullec, Jean-Yves Cascella, Kévin Ruault, Stéphanie Geffroy, Alexandre Lorieux, David Montagné, Nicolas Ollivaux, Céline Lee, Chi-Ying |
author_facet |
Toullec, Jean-Yves Cascella, Kévin Ruault, Stéphanie Geffroy, Alexandre Lorieux, David Montagné, Nicolas Ollivaux, Céline Lee, Chi-Ying |
author_sort |
Toullec, Jean-Yves |
title |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_short |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_full |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_fullStr |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_full_unstemmed |
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses |
title_sort |
antarctic krill (euphausia superba) in a warming ocean: thermotolerance and deciphering hsp70 responses |
publisher |
Springer Netherlands |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192979/ http://www.ncbi.nlm.nih.gov/pubmed/32215845 https://doi.org/10.1007/s12192-020-01103-2 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
op_source |
Cell Stress Chaperones |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7192979/ http://www.ncbi.nlm.nih.gov/pubmed/32215845 http://dx.doi.org/10.1007/s12192-020-01103-2 |
op_rights |
© Cell Stress Society International 2020 |
op_doi |
https://doi.org/10.1007/s12192-020-01103-2 |
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Cell Stress and Chaperones |
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25 |
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3 |
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519 |
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531 |
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1766266885198839808 |