Abundant and diverse arsenic‐metabolizing microorganisms in peatlands treating arsenic‐contaminated mining wastewaters
Mining operations produce large quantities of wastewater. At a mine site in Northern Finland, two natural peatlands are used for the treatment of mining‐influenced waters with high concentrations of sulphate and potentially toxic arsenic (As). In the present study, As removal and the involved microb...
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ftpubmed:oai:pubmedcentral.nih.gov:7187466 2023-05-15T17:42:38+02:00 Abundant and diverse arsenic‐metabolizing microorganisms in peatlands treating arsenic‐contaminated mining wastewaters Kujala, Katharina Besold, Johannes Mikkonen, Anu Tiirola, Marja Planer‐Friedrich, Britta 2020-02-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187466/ http://www.ncbi.nlm.nih.gov/pubmed/31984582 https://doi.org/10.1111/1462-2920.14922 en eng John Wiley & Sons, Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187466/ http://www.ncbi.nlm.nih.gov/pubmed/31984582 http://dx.doi.org/10.1111/1462-2920.14922 © 2020 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. CC-BY-NC Research Articles Text 2020 ftpubmed https://doi.org/10.1111/1462-2920.14922 2020-05-03T00:50:08Z Mining operations produce large quantities of wastewater. At a mine site in Northern Finland, two natural peatlands are used for the treatment of mining‐influenced waters with high concentrations of sulphate and potentially toxic arsenic (As). In the present study, As removal and the involved microbial processes in those treatment peatlands (TPs) were assessed. Arsenic‐metabolizing microorganisms were abundant in peat soil from both TPs (up to 10(8) cells g(dw) (−1)), with arsenate respirers being about 100 times more abundant than arsenite oxidizers. In uninhibited microcosm incubations, supplemented arsenite was oxidized under oxic conditions and supplemented arsenate was reduced under anoxic conditions, while little to no oxidation/reduction was observed in NaN(3)‐inhibited microcosms, indicating high As‐turnover potential of peat microbes. Formation of thioarsenates was observed in anoxic microcosms. Sequencing of the functional genemarkers aioA (arsenite oxidizers), arrA (arsenate respirers) and arsC (detoxifying arsenate reducers) demonstrated high diversity of the As‐metabolizing microbial community. The microbial community composition differed between the two TPs, which may have affected As removal efficiencies. In the present situation, arsenate reduction is likely the dominant net process and contributes substantially to As removal. Changes in TP usage (e.g. mine closure) with lowered water tables and heightened oxygen availability in peat might lead to re‐oxidation and re‐mobilization of bound arsenite. Text Northern Finland PubMed Central (PMC) Environmental Microbiology 22 4 1572 1587 |
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Research Articles Kujala, Katharina Besold, Johannes Mikkonen, Anu Tiirola, Marja Planer‐Friedrich, Britta Abundant and diverse arsenic‐metabolizing microorganisms in peatlands treating arsenic‐contaminated mining wastewaters |
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Research Articles |
description |
Mining operations produce large quantities of wastewater. At a mine site in Northern Finland, two natural peatlands are used for the treatment of mining‐influenced waters with high concentrations of sulphate and potentially toxic arsenic (As). In the present study, As removal and the involved microbial processes in those treatment peatlands (TPs) were assessed. Arsenic‐metabolizing microorganisms were abundant in peat soil from both TPs (up to 10(8) cells g(dw) (−1)), with arsenate respirers being about 100 times more abundant than arsenite oxidizers. In uninhibited microcosm incubations, supplemented arsenite was oxidized under oxic conditions and supplemented arsenate was reduced under anoxic conditions, while little to no oxidation/reduction was observed in NaN(3)‐inhibited microcosms, indicating high As‐turnover potential of peat microbes. Formation of thioarsenates was observed in anoxic microcosms. Sequencing of the functional genemarkers aioA (arsenite oxidizers), arrA (arsenate respirers) and arsC (detoxifying arsenate reducers) demonstrated high diversity of the As‐metabolizing microbial community. The microbial community composition differed between the two TPs, which may have affected As removal efficiencies. In the present situation, arsenate reduction is likely the dominant net process and contributes substantially to As removal. Changes in TP usage (e.g. mine closure) with lowered water tables and heightened oxygen availability in peat might lead to re‐oxidation and re‐mobilization of bound arsenite. |
format |
Text |
author |
Kujala, Katharina Besold, Johannes Mikkonen, Anu Tiirola, Marja Planer‐Friedrich, Britta |
author_facet |
Kujala, Katharina Besold, Johannes Mikkonen, Anu Tiirola, Marja Planer‐Friedrich, Britta |
author_sort |
Kujala, Katharina |
title |
Abundant and diverse arsenic‐metabolizing microorganisms in peatlands treating arsenic‐contaminated mining wastewaters |
title_short |
Abundant and diverse arsenic‐metabolizing microorganisms in peatlands treating arsenic‐contaminated mining wastewaters |
title_full |
Abundant and diverse arsenic‐metabolizing microorganisms in peatlands treating arsenic‐contaminated mining wastewaters |
title_fullStr |
Abundant and diverse arsenic‐metabolizing microorganisms in peatlands treating arsenic‐contaminated mining wastewaters |
title_full_unstemmed |
Abundant and diverse arsenic‐metabolizing microorganisms in peatlands treating arsenic‐contaminated mining wastewaters |
title_sort |
abundant and diverse arsenic‐metabolizing microorganisms in peatlands treating arsenic‐contaminated mining wastewaters |
publisher |
John Wiley & Sons, Inc. |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187466/ http://www.ncbi.nlm.nih.gov/pubmed/31984582 https://doi.org/10.1111/1462-2920.14922 |
genre |
Northern Finland |
genre_facet |
Northern Finland |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187466/ http://www.ncbi.nlm.nih.gov/pubmed/31984582 http://dx.doi.org/10.1111/1462-2920.14922 |
op_rights |
© 2020 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
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CC-BY-NC |
op_doi |
https://doi.org/10.1111/1462-2920.14922 |
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Environmental Microbiology |
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22 |
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4 |
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1572 |
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1587 |
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1766144521696968704 |