Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds
Pelagic seabirds are amongst the most threatened of all avian groups. They face a range of immunological challenges which seem destined to increase due to environmental changes in their breeding and foraging habitats, affecting prey resources and exposure to pollution and pathogens. Therefore, the i...
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ftpubmed:oai:pubmedcentral.nih.gov:7168935 2023-05-15T13:40:36+02:00 Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds Phillips, Richard A. Kraev, Igor Lange, Sigrun 2020-01-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168935/ http://www.ncbi.nlm.nih.gov/pubmed/31936359 https://doi.org/10.3390/biology9010015 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168935/ http://www.ncbi.nlm.nih.gov/pubmed/31936359 http://dx.doi.org/10.3390/biology9010015 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2020 ftpubmed https://doi.org/10.3390/biology9010015 2020-04-26T00:41:23Z Pelagic seabirds are amongst the most threatened of all avian groups. They face a range of immunological challenges which seem destined to increase due to environmental changes in their breeding and foraging habitats, affecting prey resources and exposure to pollution and pathogens. Therefore, the identification of biomarkers for the assessment of their health status is of considerable importance. Peptidylarginine deiminases (PADs) post-translationally convert arginine into citrulline in target proteins in an irreversible manner. PAD-mediated deimination can cause structural and functional changes in target proteins, allowing for protein moonlighting in physiological and pathophysiological processes. PADs furthermore contribute to the release of extracellular vesicles (EVs), which play important roles in cellular communication. In the present study, post-translationally deiminated protein and EV profiles of plasma were assessed in eight seabird species from the Antarctic, representing two avian orders: Procellariiformes (albatrosses and petrels) and Charadriiformes (waders, auks, gulls and skuas). We report some differences between the species assessed, with the narrowest EV profiles of 50–200 nm in the northern giant petrel Macronectes halli, and the highest abundance of larger 250–500 nm EVs in the brown skua Stercorarius antarcticus. The seabird EVs were positive for phylogenetically conserved EV markers and showed characteristic EV morphology. Post-translational deimination was identified in a range of key plasma proteins critical for immune response and metabolic pathways in three of the bird species under study; the wandering albatross Diomedea exulans, south polar skua Stercorarius maccormicki and northern giant petrel. Some differences in Gene Ontology (GO) biological and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for deiminated proteins were observed between these three species. This indicates that target proteins for deimination may differ, potentially contributing to a range of ... Text Antarc* Antarctic antarcticus Brown Skua Diomedea exulans Giant Petrel Stercorarius antarcticus Stercorarius maccormicki Wandering Albatross PubMed Central (PMC) Antarctic The Antarctic Biology 9 1 15 |
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Article Phillips, Richard A. Kraev, Igor Lange, Sigrun Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds |
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Article |
description |
Pelagic seabirds are amongst the most threatened of all avian groups. They face a range of immunological challenges which seem destined to increase due to environmental changes in their breeding and foraging habitats, affecting prey resources and exposure to pollution and pathogens. Therefore, the identification of biomarkers for the assessment of their health status is of considerable importance. Peptidylarginine deiminases (PADs) post-translationally convert arginine into citrulline in target proteins in an irreversible manner. PAD-mediated deimination can cause structural and functional changes in target proteins, allowing for protein moonlighting in physiological and pathophysiological processes. PADs furthermore contribute to the release of extracellular vesicles (EVs), which play important roles in cellular communication. In the present study, post-translationally deiminated protein and EV profiles of plasma were assessed in eight seabird species from the Antarctic, representing two avian orders: Procellariiformes (albatrosses and petrels) and Charadriiformes (waders, auks, gulls and skuas). We report some differences between the species assessed, with the narrowest EV profiles of 50–200 nm in the northern giant petrel Macronectes halli, and the highest abundance of larger 250–500 nm EVs in the brown skua Stercorarius antarcticus. The seabird EVs were positive for phylogenetically conserved EV markers and showed characteristic EV morphology. Post-translational deimination was identified in a range of key plasma proteins critical for immune response and metabolic pathways in three of the bird species under study; the wandering albatross Diomedea exulans, south polar skua Stercorarius maccormicki and northern giant petrel. Some differences in Gene Ontology (GO) biological and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for deiminated proteins were observed between these three species. This indicates that target proteins for deimination may differ, potentially contributing to a range of ... |
format |
Text |
author |
Phillips, Richard A. Kraev, Igor Lange, Sigrun |
author_facet |
Phillips, Richard A. Kraev, Igor Lange, Sigrun |
author_sort |
Phillips, Richard A. |
title |
Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds |
title_short |
Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds |
title_full |
Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds |
title_fullStr |
Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds |
title_full_unstemmed |
Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds |
title_sort |
protein deimination and extracellular vesicle profiles in antarctic seabirds |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168935/ http://www.ncbi.nlm.nih.gov/pubmed/31936359 https://doi.org/10.3390/biology9010015 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic antarcticus Brown Skua Diomedea exulans Giant Petrel Stercorarius antarcticus Stercorarius maccormicki Wandering Albatross |
genre_facet |
Antarc* Antarctic antarcticus Brown Skua Diomedea exulans Giant Petrel Stercorarius antarcticus Stercorarius maccormicki Wandering Albatross |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7168935/ http://www.ncbi.nlm.nih.gov/pubmed/31936359 http://dx.doi.org/10.3390/biology9010015 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/biology9010015 |
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Biology |
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9 |
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15 |
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