Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq
SIMPLE SUMMARY: As high-value fishery products, cod species are frequently faked by non-cod ones. Genetic fingerprinting is important for both certifying authenticity and traceability of fish species. In this study, we developed a method that combines DNA barcoding and restriction-site associated DN...
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ftpubmed:oai:pubmedcentral.nih.gov:7142540 2023-05-15T15:27:25+02:00 Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq Jiang, Shoujia Ma, Xingyu Li, Tao Zhu, Changqing You, Xinxin 2020-03-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142540/ http://www.ncbi.nlm.nih.gov/pubmed/32138187 https://doi.org/10.3390/ani10030423 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142540/ http://www.ncbi.nlm.nih.gov/pubmed/32138187 http://dx.doi.org/10.3390/ani10030423 © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2020 ftpubmed https://doi.org/10.3390/ani10030423 2020-04-19T00:24:45Z SIMPLE SUMMARY: As high-value fishery products, cod species are frequently faked by non-cod ones. Genetic fingerprinting is important for both certifying authenticity and traceability of fish species. In this study, we developed a method that combines DNA barcoding and restriction-site associated DNA sequencing (RAD-Seq) approach for the identification of cod products. Two sequences that contain single nucleotide polymorphism (SNP)s were identified and these SNPs can be used to distinguish three different cod species, which are Atlantic cod (Gadus morhua), Greenland turbot (Reinhardtius hippoglossoides), and Patagonian toothfish (Dissostichus eleginoides). This SNP-based method will help us to identify the products, which are sold under the name of “Xue Yu” (Cod) in China, and works in parallel with existing fish identification techniques to establish an efficient framework to detect and prevent fraud at all points of the cod commercialization. ABSTRACT: The increase in the rate of seafood fraud, particularly in the expensive fishes, forces us to verify the identity of marine products. Meanwhile, the definition of cod lacks consistency at the international level, as few standards and effective application methods are capable of accurately detecting cod species. Genetic fingerprinting is important for both certifying authenticity and traceability of fish species. In this study, we developed a method that combines DNA barcoding and the restriction-site associated DNA sequencing (RAD-Seq) approach for the identification of cod products. We first obtained 6941 high-quality single nucleotide polymorphism (SNP)s from 65.6 gigabases (Gb) of RAD-Seq raw data, and two sequences that contain SNPs were finally used to successfully identify three different cod product species, which are Atlantic cod (Gadus morhua), Greenland turbot (Reinhardtius hippoglossoides), and Patagonian toothfish (Dissostichus eleginoides). This SNP-based method will help us to identify the products, which are sold under the name of “Xue Yu” (Cod) ... Text atlantic cod Gadus morhua Greenland Patagonian Toothfish Turbot PubMed Central (PMC) Greenland Animals 10 3 423 |
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Article Jiang, Shoujia Ma, Xingyu Li, Tao Zhu, Changqing You, Xinxin Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq |
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SIMPLE SUMMARY: As high-value fishery products, cod species are frequently faked by non-cod ones. Genetic fingerprinting is important for both certifying authenticity and traceability of fish species. In this study, we developed a method that combines DNA barcoding and restriction-site associated DNA sequencing (RAD-Seq) approach for the identification of cod products. Two sequences that contain single nucleotide polymorphism (SNP)s were identified and these SNPs can be used to distinguish three different cod species, which are Atlantic cod (Gadus morhua), Greenland turbot (Reinhardtius hippoglossoides), and Patagonian toothfish (Dissostichus eleginoides). This SNP-based method will help us to identify the products, which are sold under the name of “Xue Yu” (Cod) in China, and works in parallel with existing fish identification techniques to establish an efficient framework to detect and prevent fraud at all points of the cod commercialization. ABSTRACT: The increase in the rate of seafood fraud, particularly in the expensive fishes, forces us to verify the identity of marine products. Meanwhile, the definition of cod lacks consistency at the international level, as few standards and effective application methods are capable of accurately detecting cod species. Genetic fingerprinting is important for both certifying authenticity and traceability of fish species. In this study, we developed a method that combines DNA barcoding and the restriction-site associated DNA sequencing (RAD-Seq) approach for the identification of cod products. We first obtained 6941 high-quality single nucleotide polymorphism (SNP)s from 65.6 gigabases (Gb) of RAD-Seq raw data, and two sequences that contain SNPs were finally used to successfully identify three different cod product species, which are Atlantic cod (Gadus morhua), Greenland turbot (Reinhardtius hippoglossoides), and Patagonian toothfish (Dissostichus eleginoides). This SNP-based method will help us to identify the products, which are sold under the name of “Xue Yu” (Cod) ... |
format |
Text |
author |
Jiang, Shoujia Ma, Xingyu Li, Tao Zhu, Changqing You, Xinxin |
author_facet |
Jiang, Shoujia Ma, Xingyu Li, Tao Zhu, Changqing You, Xinxin |
author_sort |
Jiang, Shoujia |
title |
Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq |
title_short |
Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq |
title_full |
Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq |
title_fullStr |
Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq |
title_full_unstemmed |
Developing Single Nucleotide Polymorphisms for Identification of Cod Products by RAD-Seq |
title_sort |
developing single nucleotide polymorphisms for identification of cod products by rad-seq |
publisher |
MDPI |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142540/ http://www.ncbi.nlm.nih.gov/pubmed/32138187 https://doi.org/10.3390/ani10030423 |
geographic |
Greenland |
geographic_facet |
Greenland |
genre |
atlantic cod Gadus morhua Greenland Patagonian Toothfish Turbot |
genre_facet |
atlantic cod Gadus morhua Greenland Patagonian Toothfish Turbot |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142540/ http://www.ncbi.nlm.nih.gov/pubmed/32138187 http://dx.doi.org/10.3390/ani10030423 |
op_rights |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/ani10030423 |
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Animals |
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