Amino acid δ(15)N underestimation of cetacean trophic positions highlights limited understanding of isotopic fractionation in higher marine consumers
Compound‐specific stable isotope analysis (CSIA) of amino acids (AAs) has been rapidly incorporated in ecological studies to resolve consumer trophic position (TP). Differential (15)N fractionation of “trophic” AAs, which undergo trophic (15)N enrichment, and “source” AAs, which undergo minimal trop...
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ftpubmed:oai:pubmedcentral.nih.gov:7141024 2023-05-15T15:41:56+02:00 Amino acid δ(15)N underestimation of cetacean trophic positions highlights limited understanding of isotopic fractionation in higher marine consumers Matthews, Cory J. D. Ruiz‐Cooley, Rocio I. Pomerleau, Corinne Ferguson, Steven H. 2020-03-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141024/ https://doi.org/10.1002/ece3.6142 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141024/ http://dx.doi.org/10.1002/ece3.6142 © 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Original Research Text 2020 ftpubmed https://doi.org/10.1002/ece3.6142 2020-04-12T01:04:49Z Compound‐specific stable isotope analysis (CSIA) of amino acids (AAs) has been rapidly incorporated in ecological studies to resolve consumer trophic position (TP). Differential (15)N fractionation of “trophic” AAs, which undergo trophic (15)N enrichment, and “source” AAs, which undergo minimal trophic (15)N enrichment and serve as a proxy for primary producer δ(15)N values, allows for internal calibration of TP. Recent studies, however, have shown the difference between source and trophic AA δ(15)N values in higher marine consumers is less than predicted from empirical studies of invertebrates and fish. To evaluate CSIA‐AA for estimating TP of cetaceans, we compared source and trophic AA δ(15)N values of multiple tissues (skin, baleen, and dentine collagen) from five species representing a range of TPs: bowhead whales, beluga whales, short‐beaked common dolphins, sperm whales, and fish‐eating (FE) and marine mammal‐eating (MME) killer whale ecotypes. TP estimates (TP(CSIA)) using several empirically derived equations and trophic discrimination factors (TDFs) were 1–2.5 trophic steps lower than stomach content‐derived estimates (TP(SC)) for all species. Although TP(CSIA) estimates using dual TDF equations were in better agreement with TP(SC) estimates, our data do not support the application of universal or currently available dual TDFs to estimate cetacean TPs. Discrepancies were not simply due to inaccurate TDFs, however, because the difference between consumer glutamic acid/glutamine (Glx) and phenylalanine (Phe) δ(15)N values (δ(15)N(Glx‐Phe)) did not follow expected TP order. In contrast to pioneering studies on invertebrates and fish, our data suggest trophic (15)N enrichment of Phe is not negligible and should be examined among the potential mechanisms driving “compressed” and variable δ(15)N(Glx‐Phe) values at high TPs. We emphasize the need for controlled diet studies to understand mechanisms driving AA‐specific isotopic fractionation before widespread application of CSIA‐AA in ecological studies of ... Text Beluga Beluga* Killer Whale Killer whale PubMed Central (PMC) Ecology and Evolution 10 7 3450 3462 |
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Original Research Matthews, Cory J. D. Ruiz‐Cooley, Rocio I. Pomerleau, Corinne Ferguson, Steven H. Amino acid δ(15)N underestimation of cetacean trophic positions highlights limited understanding of isotopic fractionation in higher marine consumers |
topic_facet |
Original Research |
description |
Compound‐specific stable isotope analysis (CSIA) of amino acids (AAs) has been rapidly incorporated in ecological studies to resolve consumer trophic position (TP). Differential (15)N fractionation of “trophic” AAs, which undergo trophic (15)N enrichment, and “source” AAs, which undergo minimal trophic (15)N enrichment and serve as a proxy for primary producer δ(15)N values, allows for internal calibration of TP. Recent studies, however, have shown the difference between source and trophic AA δ(15)N values in higher marine consumers is less than predicted from empirical studies of invertebrates and fish. To evaluate CSIA‐AA for estimating TP of cetaceans, we compared source and trophic AA δ(15)N values of multiple tissues (skin, baleen, and dentine collagen) from five species representing a range of TPs: bowhead whales, beluga whales, short‐beaked common dolphins, sperm whales, and fish‐eating (FE) and marine mammal‐eating (MME) killer whale ecotypes. TP estimates (TP(CSIA)) using several empirically derived equations and trophic discrimination factors (TDFs) were 1–2.5 trophic steps lower than stomach content‐derived estimates (TP(SC)) for all species. Although TP(CSIA) estimates using dual TDF equations were in better agreement with TP(SC) estimates, our data do not support the application of universal or currently available dual TDFs to estimate cetacean TPs. Discrepancies were not simply due to inaccurate TDFs, however, because the difference between consumer glutamic acid/glutamine (Glx) and phenylalanine (Phe) δ(15)N values (δ(15)N(Glx‐Phe)) did not follow expected TP order. In contrast to pioneering studies on invertebrates and fish, our data suggest trophic (15)N enrichment of Phe is not negligible and should be examined among the potential mechanisms driving “compressed” and variable δ(15)N(Glx‐Phe) values at high TPs. We emphasize the need for controlled diet studies to understand mechanisms driving AA‐specific isotopic fractionation before widespread application of CSIA‐AA in ecological studies of ... |
format |
Text |
author |
Matthews, Cory J. D. Ruiz‐Cooley, Rocio I. Pomerleau, Corinne Ferguson, Steven H. |
author_facet |
Matthews, Cory J. D. Ruiz‐Cooley, Rocio I. Pomerleau, Corinne Ferguson, Steven H. |
author_sort |
Matthews, Cory J. D. |
title |
Amino acid δ(15)N underestimation of cetacean trophic positions highlights limited understanding of isotopic fractionation in higher marine consumers |
title_short |
Amino acid δ(15)N underestimation of cetacean trophic positions highlights limited understanding of isotopic fractionation in higher marine consumers |
title_full |
Amino acid δ(15)N underestimation of cetacean trophic positions highlights limited understanding of isotopic fractionation in higher marine consumers |
title_fullStr |
Amino acid δ(15)N underestimation of cetacean trophic positions highlights limited understanding of isotopic fractionation in higher marine consumers |
title_full_unstemmed |
Amino acid δ(15)N underestimation of cetacean trophic positions highlights limited understanding of isotopic fractionation in higher marine consumers |
title_sort |
amino acid δ(15)n underestimation of cetacean trophic positions highlights limited understanding of isotopic fractionation in higher marine consumers |
publisher |
John Wiley and Sons Inc. |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141024/ https://doi.org/10.1002/ece3.6142 |
genre |
Beluga Beluga* Killer Whale Killer whale |
genre_facet |
Beluga Beluga* Killer Whale Killer whale |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141024/ http://dx.doi.org/10.1002/ece3.6142 |
op_rights |
© 2020 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/ece3.6142 |
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Ecology and Evolution |
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10 |
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7 |
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3450 |
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3462 |
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1766374804625031168 |